Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Yanik, Mehmet Fatih; Rohde, Christopher; Pardo-Martín, Carlos

doi:10.1146/annurev-bioeng-071910-124703

Cited by 63 publications

(49 citation statements)

References 166 publications

(217 reference statements)

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“…During the application of this method, liquid infection assays are set up in 96- or 384-well plate formats. Automated systems can supply the plates with the liquid media, nematodes, and specific quantities of compounds [14]. Thus, the testing of thousands of candidate compounds is accelerated.…”

Section: Which System Is More Appropriate For Studying Antimicrobial mentioning

confidence: 99%

Selecting an Invertebrate Model Host for the Study of Fungal Pathogenesis

2012

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Section: Which System Is More Appropriate For Studying Antimicrobial mentioning

confidence: 99%

Selecting an Invertebrate Model Host for the Study of Fungal Pathogenesis

2012

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“…With the advent of polydimethylsiloxane (PDMS)-based microfluidics, a new generation of immobilization devices has emerged (Lockery et al, 2008; Mannik et al, 2009; Westendorf et al, 2010; Wang et al, 2011; Yanik et al, 2011). While not as optically clear as glass, the PDMS polymer is transparent and is also gas permeable, so the specimen can remain visible and viable.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic-Enabled Mechanical Microcompressor for the Immobilization of Live Single- and Multi-Cellular Specimens

et al. 2014

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A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.

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“…A clear goal for future work is to develop nano-SPEARs that can perform voltage and current clamp experiments to measure ion channel behavior and postsynaptic potentials. We also envision that future work will combine the high-throughput, versatile electrophysiology of nano-SPEARs with other high-throughput assays 48 to help uncover fundamental relationships between genetics, electrophysiology, and behavior using small model organisms.…”

Section: Discussionmentioning

confidence: 99%

Scalable electrophysiology in intact small animals with nanoscale suspended electrode arrays

et al. 2017

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Electrical measurements from large populations of animals would help reveal fundamental properties of the nervous system and neurological diseases. Small invertebrates are ideal for these large-scale studies; however, patch-clamp electrophysiology in microscopic animals typically requires low-throughput and invasive dissections. To overcome these limitations, we present nano-SPEARs: suspended electrodes integrated into a scalable microfluidic device. Using this technology, we have made the first extracellular recordings of body-wall muscle electrophysiology inside an intact roundworm, Caenorhabditis elegans. We can also use nano-SPEARs to record from multiple animals in parallel and even from other species, such as Hydra littoralis. Furthermore, we use nano-SPEARs to establish the first electrophysiological phenotypes for C. elegans models for Amyotrophic Lateral Sclerosis and Parkinson’s disease, and show a partial rescue of the Parkinson’s phenotype through drug treatment. These results demonstrate that nano-SPEARs provide the core technology for microchips that enable scalable, in vivo studies of neurobiology and neurological diseases.

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Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Cited by 63 publications

References 166 publications

Selecting an Invertebrate Model Host for the Study of Fungal Pathogenesis

Selecting an Invertebrate Model Host for the Study of Fungal Pathogenesis

A Microfluidic-Enabled Mechanical Microcompressor for the Immobilization of Live Single- and Multi-Cellular Specimens

Scalable electrophysiology in intact small animals with nanoscale suspended electrode arrays

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