Near‐infrared spectroscopy allows detection and species identification of <i>Epichloë</i> endophytes in <i>Lolium perenne</i>

Soto-Barajas, Milton C.; Zabalgogeazcoa, Íñigo; González-Martı́n, I.; Aldana, Beatriz R. Vázquez de

doi:10.1002/jsfa.9038

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…In addition, a discrimination between plants infected by M1 and M3 morphotypes was possible by means of near infrared reflectance spectroscopy (NIRS) (Soto- Barajas et al, 2018). Whether M1 and M3 isolates belong to different species is a question that deserves further research.…”

Section: Discussionmentioning

confidence: 99%

Sympatric Epichloë species and chemotypic profiles in natural populations of Lolium perenne

2019

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The distribution of different Epichloë species within eight natural populations of Lolium perenne was studied. In total, 40.2% of the asymptomatic plants were infected by Epichloë festucae var. lolii or by Epichloë typhina. Both species occurred in sympatry at seven populations, and some plants had dual infections by both taxa. No hybrid taxa such as Epichloë hybrida were detected. Epichloë festucae strains were classified into two morphotypes, M1 and M3, according to culture characters, both morphotypes occurred in sympatry at seven populations. Plants bearing stromata produced by Epichloë typhina were observed, but asymptomatic plants infected by this species also occurred at seven populations. The alkaloid profile of Lolium perenne plants was related to the morphotype of their infecting strains: Most plants infected by M3-strains were characterized by lolitrem, and those with M1-strains contained either ergovaline or lolitrem. Plants infected by Epichloë typhina were characterized by high peramine content.

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Section: Discussionmentioning

confidence: 99%

Sympatric Epichloë species and chemotypic profiles in natural populations of Lolium perenne

2019

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“…Most of these analyses predict aspects about forage composition in terms of chemical fractions such as crude protein, neutral-detergent fiber, acid-detergent fiber, water-soluble carbohydrates, lignin and dry-matter digestibility [6][7][8] and recently other parameters such as alkaloids [9]. Profiling forage grass cultivars at all these traits gives breeders valuable information on which they can base their selection for elite varieties with a high digestibility [10] or particular characteristics such as alkaloid profile [9,11].…”

Section: Introductionmentioning

confidence: 99%

Determination of Loline Alkaloids and Mycelial Biomass in Endophyte-Infected Schedonorus pratensis by Near-Infrared Spectroscopy and Chemometrics

et al. 2020

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Near infrared spectroscopy (NIRS) is an accurate, fast and nondestructive technique whose use in predicting forage quality has become increasingly relevant in recent decades. Epichloë-infected grass varieties are commonly used in areas with high pest pressure due to their better performances compared to endophyte-free varieties. The insect resistance of Epichloë-infected grasses has been associated with four main groups of endophyte secondary metabolites: ergot alkaloids, indole-diterpenes, lolines and peramine. Concentrations of these alkaloids are usually measured with high performance liquid chromatography or gas chromatography analysis, which are accurate methods but relatively expensive and laborious. In this paper, we developed a rapid method based on NIRS to detect and quantify loline alkaloids in wild accessions of Schedonorus pratensis infected with the fungal endophyte Epichloë uncinata. The quantitative NIR equations obtained by modified partial least squares algorithm had coefficients of correlation of 0.90, 0.78, 0.85, 0.90 for N-acetylloline, N-acetylnorloline and N-formylloline and the sum of the three, respectively. The acquired NIR spectra were also used for developing an equation to predict in planta fungal biomass with a coefficient of correlation of 0.75. These results showed that the use of NIRS and chemometrics allows the quantification of loline alkaloids and mycelial biomass in a heterogeneous set of endophyte-infected meadow fescue samples.

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Water Distillation Extraction of Essential Oil from Sideritis Raeseri Herb

et al. 2019

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Near‐infrared spectroscopy allows detection and species identification of Epichloë endophytes in Lolium perenne

Abstract: Near-infrared reflectance spectroscopy can detect the presence of Epichloë fungal endophytes directly in samples of perennial ryegrass, and it is adequate for discriminating among fungal species. © 2018 Society of Chemical Industry.

Cited by 4 publications

References 46 publications

Sympatric Epichloë species and chemotypic profiles in natural populations of Lolium perenne

Sympatric Epichloë species and chemotypic profiles in natural populations of Lolium perenne

Determination of Loline Alkaloids and Mycelial Biomass in Endophyte-Infected Schedonorus pratensis by Near-Infrared Spectroscopy and Chemometrics

Water Distillation Extraction of Essential Oil from Sideritis Raeseri Herb

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