Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

Yan, Ling; Gu, Qing-Song; Jiang, Wenli; Tan, Min; Tan, Zhi-Ke; Mao, Guojiang; Xu, Fei; Li, Chun‐Yan

doi:10.1021/acs.analchem.1c04169

Cited by 86 publications

(35 citation statements)

References 48 publications

(61 reference statements)

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“…Fluorescence technology has the advantages of good selectivity, high sensitivity, high imaging resolution, and noninvasiveness, which makes it a popular tool in the detection and imaging of biological related fields. − Previously, many fluorescent probes for single detection of ATP or ONOO – in cancer have been reported. , However, in the complex TME, only detecting a single substance cannot meet the needs of high accuracy, because it may cause “false positive” or “false negative” results. , Therefore, it is imperative to design a tool that can detect multiple cancer markers with multiple channels or multiple signals.…”

Section: Introductionmentioning

confidence: 99%

A Dual-Channel Fluorescent Nanoprobe for Sequential Detection of ATP and Peroxynitrite to Accurately Distinguish between Normal Cells and Cancer Cells

et al. 2022

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Cancer is one of the biggest public enemies of global health with its high morbidity and mortality. Achieving early diagnosis is the most effective means of reducing cancer harm, which requires the use of powerful tools to accurately identify biomarkers. However, most of the reported fluorescent probes for cancer diagnosis can only detect one substance, which makes it difficult to meet the requirements of high accuracy. Here, a fluorescent nanoprobe (CPQ@ZIF-90) for sequential detection of ATP and ONOO − is constructed by encapsulating the ONOO − sensitive unit CPQ within ZIF-90. CPQ@ZIF-90 first reacts with ATP to release CPQ, which greatly enhances the fluorescence at 740 nm. Then, the released CPQ continues to react with ONOO − and is oxidatively cleaved by ONOO − to form a coumarin product with a small π-conjugated structure, which significantly enhances the fluorescence at 510 nm. CPQ@ZIF-90 shows high sensitivity and selectivity for the detection of ATP and then ONOO − . Moreover, CPQ@ZIF-90 has good biocompatibility and successfully realizes the sequential detection of a dual-channel fluorescence change of ATP and ONOO − in living cells and zebrafish and accurately distinguishes normal cells from cancer cells. CPQ@ZIF-90 is expected to be a potential tool for accurate cancer diagnosis through sequential detection of two cancer markers.

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Section: Introductionmentioning

confidence: 99%

A Dual-Channel Fluorescent Nanoprobe for Sequential Detection of ATP and Peroxynitrite to Accurately Distinguish between Normal Cells and Cancer Cells

et al. 2022

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“…Until now, a number of pH-activatable fluorescent probes have been developed for tumor imaging, but none of them was able to visualize and differentiate tumor and NAFLD simultaneously, owing to the different physiological pH in the tumor (acidic pH) and liver (neutral to slightly basic pH). ,− Typically, single-responsive fluorescent probes cannot be used for distinguishing two different diseases because of fluorescence generation in the same channel. Dual-responsive fluorescent probes hold great potential for multi-disease diagnosis in a dual-color imaging mode, while most of them are limited to cellular imaging study due to the short absorption/emission wavelengths, spectral overlap, and slow response time. ,− To the best of our knowledge, there have been no reports using pH-/viscosity-responsive NIR-II fluorescent probes for simultaneous detection of NAFLD and metastatic intestinal cancer. We envisage that the development of bichromatic fluorophores (BCFluors) with both NIR-I and NIR-II imaging capability is a key issue for dual-disease diagnosis in vivo but remains challenging.…”

Section: Introductionmentioning

confidence: 99%

Bichromatic Imaging with Hemicyanine Fluorophores Enables Simultaneous Visualization of Non-alcoholic Fatty Liver Disease and Metastatic Intestinal Cancer

Liu

Chen

et al. 2022

Anal. Chem.

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Simultaneous detection of different diseases via a single fluorophore is challenging. We herein report a bichromatic fluorophore named Cy-914 for the simultaneous diagnosis of non-alcoholic fatty liver disease (NAFLD) and metastatic intestinal cancer by leveraging its NIR-I/NIR-II dualcolor imaging capability. Cy-914 with a pK a of 6.98 exhibits high sensitivity to pH and viscosity, showing turn-on NIR-I fluorescence at 795 nm in an acidic tumor microenvironment, meanwhile displaying intense NIR-II fluorescence at 914/1030 nm under neutral to slightly basic viscous conditions. Notably, Cy-914 could sensitively and noninvasively monitor viscosity variations in the progression of NAFLD. More importantly, it was able to simultaneously visualize NAFLD (ex/em = 808/1000−1700 nm) and intestinal metastases (ex/em = 570/810−875 nm) in two independent channels without spectral cross interference after topical spraying, further improving fluorescence-guided surgery of tiny metastases less than 3 mm. This strategy may provide an understanding for developing multi-color fluorophores for multidisease diagnosis.

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“…Fluorescence imaging is a noninvasive method, − which is conducive to detecting and monitoring the variation of in situ biomarkers in a real-time manner in plants. − As for in vivo fluorescence imaging in plants, the imaging quality in the shorter wavelength range (e.g., visible light (400–700 nm) or NIR-I range (700–900 nm)) is usually compromised or eroded because such pigments as chlorophylls, anthocyanins, and carotenoids in plants usually have absorptions in the visible-light wavelength range and exhibit fluorescence in the visible-light or NIR-I wavelength range. − However, near-infrared second window (NIR-II, emission: 900–1700 nm) fluorescence imaging can achieve much superior imaging performance, − owing to no interference from plant-related pigments in the NIR-II wavelength range.…”

Section: Introductionmentioning

confidence: 99%

Water-Dispersible Activatable Nanoprobe for Detecting Cadmium-Ion-Induced Oxidative Stress in Edible Crops via Near-Infrared Second-Window Fluorescence Imaging

et al. 2022

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Edible crops are important in terms of food security and sustainable agriculture. Heavy-metal-ion contamination of water/soil has deleterious impacts on the growth of edible crops. Among the heavy metals, cadmium (Cd) is toxic to plants, people, and animals, as it is widely used in industry; it has become the most important metal ion in the soil/water pollution. Once the toxic Cd ion enters edible crops via the water/soil in which the crops grow, it will induce oxidative stress (overproduction of reactive oxygen species with H2O2 being the most abundant) in the crops, and strong oxidative stress leads to the crops’ growth depression or inhibition. Hence, it is of great significance to accurately monitor the oxidative stress induced by Cd ions in edible crops, as the monitoring results could be employed for the early warning of Cd-ion pollution in water/soil. Herein, we design an activatable nanoprobe that can detect Cd-ion-induced oxidative stress in edible crops via near-infrared second-window (NIR-II) fluorescence imaging. The molecular probe IXD-B contains the diphenylamine-modified xanthene group acting as the electron-donating unit, bis(methylenemalononitrile)indan as the electron-accepting unit, and the methenephenylboronic acid group as the recognition moiety for H2O2 and the fluorescence quencher. The probe molecules being encapsulated by the amphiphilic DSPE-PEG2000 render the water-dispersible nanoprobe (IXD-B@DSPE-PEG2000). When the nanoprobe enters the edible crops, it can be activated by the overexpressed H2O2 therein and consequently emit strong NIR-II fluorescence signals for visualizing and tracking the oxidative stress in edible crops induced by Cd ions.

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Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

Cited by 86 publications

References 48 publications

A Dual-Channel Fluorescent Nanoprobe for Sequential Detection of ATP and Peroxynitrite to Accurately Distinguish between Normal Cells and Cancer Cells

A Dual-Channel Fluorescent Nanoprobe for Sequential Detection of ATP and Peroxynitrite to Accurately Distinguish between Normal Cells and Cancer Cells

Bichromatic Imaging with Hemicyanine Fluorophores Enables Simultaneous Visualization of Non-alcoholic Fatty Liver Disease and Metastatic Intestinal Cancer

Water-Dispersible Activatable Nanoprobe for Detecting Cadmium-Ion-Induced Oxidative Stress in Edible Crops via Near-Infrared Second-Window Fluorescence Imaging

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