Harding (2007) Dynamic light scattering as a relative tool for assessing the molecular integrity and stability of monoclonal antibodies, Biotechnology and Genetic Engineering Reviews, 24:1, 117-128,

Triple-negative breast cancer (TNBC), an aggressive subtype of breast cancer with higher rates of early relapse and metastasis, is frequently associated with aberrant activation of epithelial-mesenchymal transition (EMT). Nonetheless, how EMT is initiated and regulated during TNBC progression is not well understood. Here, we report that NUMB is a negative regulator of EMT in both human mammary epithelial cells and breast cancer cells. Reduced NUMB expression was significantly associated with elevated EMT in TNBC. Conversely, overexpression of NUMB strongly attenuated the EMT program and metastasis of TNBC cell lines. Interestingly, we showed that NUMB employs different molecular mechanisms to regulate EMT. In normal mammary epithelial cells and breast cancer cells expressing wild-type p53, NUMB suppressed EMT by stabilizing p53. However, in TNBC cells, loss of NUMB facilitated the EMT program by activating Notch signaling. Consistent with these findings, low NUMB expression and high Notch activity were significantly correlated with the TNBC subtype in patients. Collectively, these findings reveal novel molecular mechanisms of NUMB in the regulation of breast tumor EMT, especially in TNBC.

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The cloud-enabled Internet of controlled things (IoCT) envisions a network of sensors, controllers, and actuators connected through a local cloud in order to intelligently control physical devices. Because cloud services are vulnerable to advanced persistent threats (APTs), each device in the IoCT must strategically decide whether to trust cloud services that may be compromised. In this paper, we present iSTRICT, an interdependent strategic trust mechanism for the cloud-enabled IoCT. iSTRICT is composed of three interdependent layers. In the cloud layer, iSTRICT uses FlipIt games to conceptualize APTs. In the communication layer, it captures the interaction between devices and the cloud using signaling games. In the physical layer, iSTRICT uses optimal control to quantify the utilities in the higher level games. Best response dynamics link the three layers in an overall "game-of-games," for which the outcome is captured by a concept called Gestalt Nash equilibrium (GNE). We prove the existence of a GNE under a set of natural assumptions and develop an adaptive algorithm to iteratively compute the equilibrium. Finally, we apply iSTRICT to trust management for autonomous vehicles that rely on measurements from remote sources. We show that strategic trust in the communication layer achieves a worst-case probability of compromise for any attack and defense costs in the cyber layer.

Abstract-The integration of microgrids that depend on the renewable distributed energy resources with the current power systems is a critical issue in the smart grid. In this paper, we propose a non-cooperative game-theoretic framework to study the strategic behavior of distributed microgrids that generate renewable energies and characterize the power generation solutions by using the Nash equilibrium concept. Our framework not only incorporates economic factors but also takes into account the stability and efficiency of the microgrids, including the power flow constraints and voltage angle regulations. We develop two decentralized update schemes for microgrids and show their convergence to a unique Nash equilibrium. Also, we propose a novel fully distributed PMU-enabled algorithm which only needs the information of voltage angle at the bus. To show the resiliency of the distributed algorithm, we introduce two failure models of the smart grid. Case studies based on the IEEE 14-bus system are used to corroborate the effectiveness and resiliency of the proposed algorithms.