Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells

Gholami, Leila; Hendi, Seyedeh Sareh; Saidijam, Massoud; Mahmoudi, Roghayeh; Tarzemany, Rana; Arkian, Aliasghar; Afshar, Saeid; Fekrazad, Reza

doi:10.1007/s10103-021-03282-5

Cited by 15 publications

(11 citation statements)

References 72 publications

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“…The energy density selection criteria was based on the average of the most widely used energy density as indicated in systematic reviews [ 23 , 26 , 29 , 30 ]. Additionally, we hypothesized that the energy density of 4 J/cm2 would be more effective based on these articles [ 31 – 33 ]. As a final note, The results of our study were consistent with those of the articles cited above.…”

Section: Discussionmentioning

confidence: 99%

Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla

et al. 2022

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Background This experimental study aimed to assess the effect of irradiation of red light-emitting diode (LED) and Diode low-level laser (LLL) on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs). Materials and methods SCAPs were isolated from the human tooth root. The experimental groups were subjected to 4 J/cm2 diode low level laser and red LED irradiation in osteogenic medium. The control group did not receive any irradiation. Cell viability/proliferation of SCAPs was assessed by the methyl thiazolyl tetrazolium (MTT) assay on days 1 and 2 (n = 9). Osteogenic differentiation was evaluated by alizarin red staining (ARS) (n = 3), and expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR) (n = 12) on days 1 and 2. SPSS version 18 was used for data evaluation. The Kruskal-Wallis and Mann-Whitney tests were used to compare the groups at each time point. Results The MTT assay showed no significant difference in cell viability/proliferation of SCAPs in the low level laser, red LED, and control groups at 24 or 48 h (P < 0.001). The ARS assessment showed that low level laser and red LED irradiation enhanced osteogenic differentiation of SCAPs. low level laser and red LED irradiation both induced over-expression of osteogenic/dentinogenic genes including alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) in SCAPs. Up-regulation of genes was significantly greater in low level laser irradiation group than red LED group (P < 0.001). Conclusion Diode low level laser irradiation with 4 J/cm2 energy density and red LED irradiation enhanced osteogenic differentiation of SCAPs without adversely affecting cell viability.

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Section: Discussionmentioning

confidence: 99%

Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla

et al. 2022

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“…At day 21, the differential microenvironment was removed and the cells were washed with PBS. The cells were lysed with Trypsin–EDTA (0.25%) and lysate buffer 60 (µL) and Bradford assay was also performed to evaluate their protein content [ 30 ]. In order to investigate the alkaline phosphatase activity, 25 g of protein from each sample along with alkaline phosphatase kit solutions were read-out at 405 nm using the Elisa reader.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of apical papilla cells from root end of human third molar and their differentiation into cementoblast cells: an in vitro study

et al. 2023

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Background Periodontal regeneration, treatment of periodontal-related diseases and improving the function of implants are global therapeutic challenges. The differentiation of human stem cells from apical papilla into cementoblasts may provide a strategy for periodontitis treatment. This study aimed to evaluate the differentiation of primary human stem cells apical papilla (hSCAPs) to cementoblast cells. Material and methods SCAPs cells were isolated from human third molar and then incubated for 21 days in a differentiation microenvironment. Alkaline phosphatase (ALP) and Alizarin red S staining assays were performed to evaluate the calcium deposition and formation of hydroxyapatite in the cultured hSCAPs microenvironment. Real-time polymerase chain reaction (RT-PCR) assay was performed for cementum protein 1 (CEMP1), collagen type I (COL1), F-Spondin (SPON1), osteocalcin (OCN), and osteopontin (OPN) as specific markers of cementoblasts and their progenitors. Results ALP phosphatase activity in day 21 of treatment demonstrated a significant increase in ALP compared to the control. Alizarin red S staining assay showed that the differentiated hSCAPs offered a great amount of calcium deposition nodules compared to the control. The increased expression level of CEMP1, OCN, OPN, COL1 and Spon1 was observed in days 7, 14 and 21 compared to the control, while greatest expression level was observed in day 21. Conclusion In conclusion, the differentiation microenviroment is convenient and useful for promoting the differentiation of hSCAPs into cementoblast.

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“…The 940 nm laser irradiation effects on cells have been investigated in a few studies. In a prior study, Gholami et al (57) investigated the effects of the similar 940 nm wavelength (4 J cm −2 , 100 mW) on stem cells derived from periodontal ligaments. Their results showed that PBM treatments with a CW source slightly increased cell survival at 48 and 72 h but were not statistically significant (P > 0.05).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Pulsed and Continuous Wave Diode Laser at 940 nm on the Viability and Migration of Gingival Fibroblasts

Jazaeri

Torkzaban

Afshar

et al. 2022

Photochem & Photobiology

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Gingival fibroblasts have critical roles in oral wound healing. Photobiomodulation (PBM) has been shown to promote mucosal healing and is now recommended for managing oncotherapy‐associated oral mucositis. This study examined the effects of the emission mode of a 940 nm diode laser on the viability and migration of human gingival fibroblasts. Cells were cultured in a routine growth media and treated with PBM (average power 0.1 W cm−2, average fluence 3 J cm−2, every 12 h for six sessions) in one continuous wave and two pulsing settings with 20% and 50% duty cycles. Cell viability was assessed using MTT, and digital imaging quantified cell migration. After 48 and 72 h, all treatment groups had significantly higher viability (n = 6, P < 0.05) compared with the control. The highest viability was seen in the pulsed (20% duty cycle) group at the 72‐h time point. PBM improved fibroblast migration in all PBM‐treated groups, but differences were not statistically significant (n = 2, P > 0.05). PBM treatments can promote cell viability in both continuous and pulsed modes. Further studies are needed to elucidate the optimal setting for PBM‐evoked responses for its rationalized use in promoting specific phases of oral wound healing.

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Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells

Cited by 15 publications

References 72 publications

Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla

Effect of diode low level laser and red light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla

Isolation and characterization of apical papilla cells from root end of human third molar and their differentiation into cementoblast cells: an in vitro study

Comparison of Pulsed and Continuous Wave Diode Laser at 940 nm on the Viability and Migration of Gingival Fibroblasts

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