Naturally occurring polyphenolic antioxidants modulate IgE‐mediated mast cell activation

Abstract

SUMMARYReactive oxygen species (ROS) are known to modulate activities of a host of kinases, phosphatases and transcription factors. Rutin and chlorogenic acid (CGA) are the major polyphenolic antioxidants present in the small molecular fraction of smokeless tobacco leaf extracts, as ascertained by reverse-phase high-pressure liquid chromatography (HPLC) and mass spectrometry. Levels of intracellular ROS in resting versus antigen±immunoglobulin E (IgE)-challenged murine mast cells were measured at 510 nm by¯uorescence-activated cell sorting (FACS) using carboxydichloro¯uorescein (DCFH-DA). Enhanced ROS production was observed in IgE-sensitized mast cells following antigenic challenge. Rutin and CGA reduced ROS levels in antigen±IgE-activated mast cells. Concomitantly, they also profoundly inhibited histamine release by these activated mast cells. In contrast, rutin and CGA augmented the inducible cytokine messages, i.e. interleukin (IL)-10, IL-13, interferon-c (IFN-c), IL-6 and tumour necrosis factor-a (TNF-a) in IgE-sensitized mast cells following antigen challenge. This study indicates that tobacco polyphenolic antioxidants that quench intracellular ROS, differentially affect two effector functions of antigen±IgE-activated mast cells. This model system may be employed to determine the molecular target of polyphenols. The potential role of these polyphenolic antioxidants on IgE-mediated allergy in vivo depends on a balance of their differential effects on mast cell activation.

FcεRI Signaling of Mast Cells Activates Intracellular Production of Hydrogen Peroxide: Role in the Regulation of Calcium Signals

“…The fact that ebselen, which shows glutathione peroxidase-like activity, also suppresses LTC 4 secretion supports this idea. Unlike DPI, ebselen has little effect on the release of preformed granular mediators such as histamine and ␤-hexosaminidase, although also these two responses were shown to be ROS sensitive previously (22,25,26,44,45) and in the present work. These observations indicate that another species of oxidant whose production is sensitive to DPI treatment might be more important than H 2 O 2 in regulating degranulation.…”

Protective effects ofHammada scopariaflavonoid-enriched fraction on liver injury induced by warm ischemia/reperfusion

“…However, the most potent compound was rutin (Bourogaa et al, 2011). The latter is a powerful antioxidant and anti-inflammatory polyphenol (Chen et al, 2000). It was suggested that the reduction of ROS production of rutin is related to the enhancement of (SOD, GPx, or CAT activity as well as of its inhibitory activity on xanthine oxidase, which is an important enzyme in the oxidative injury of tissues (Khan et al, 2009;Wang et al, 2012).…”

Chemo Preventive Effect of Rutin Against N-Nitrosodiethylamine-Induced and Phenobarbital- Promoted Hepatocellular Carcinoma in Wistar Rats

“…6 Rutin (3′,4′,5,7-tetrahydroxy-flavone-3-rutinoside) (Figure 1a), a common dietary flavonoid with a wide range of pharmacological activities is present in many plants (as buckwheat seeds, tea), fruits (citrus fruits, apple), vegetables (onion), and red wine etc,. 7 Different studies have represented the biological effects of Rutin, such as anti-oxidative, antiinflammatory, antihypertensive, anti-carcinogenic, cytoprotective, and cardio protective activities. Figure 1b is found in the environment, and it is present in foods, beverages, tobacco, smoke, agricultural chemicals, cosmetics and industrial pollution.…”