Protective effects ofHammada scopariaflavonoid-enriched fraction on liver injury induced by warm ischemia/reperfusion

Abstract

Context: Hepatic ischemia/reperfusion injury (IRI) is a major cause of liver damage during liver surgery and transplantation. Plants have historically been used in treating liver damage, and Hammada scoparia (Pomel) (Chenopodiaceae) has been reported to possess a broad spectrum of pharmacological and therapeutic activities. Objective: In this study, a flavonoid-enriched fraction was used before the warm ischemia (WI) process as pharmacological preconditioning and in combination with technical postconditioning to evaluate their protective effects. Materials and methods: The rats were divided into five groups: a sham group; a control group (Control-IR) that was submitted to 60 min WI; a Pharmacological Preconditioning group (PreC-IR) that received flavonoid-enriched fraction (200 mg/kg body weight); a Postconditioning group (PostC) and a PreC + PostC group. Results: The use of the flavonoid-enriched fraction was noted to significantly (p50.05) reduce liver injury, as evidenced by the decrease in liver transaminase activities (AST and ALT) and lactic dehydrogenase (LDH), alkaline phosphatase (ALP), and lipid peroxidation (TBARS), levels as well as the enhancement of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) responses. The results also indicated that, compared with the separate application of pharmacological preconditioning and postconditioning, the combination of both treatments was more effective in reducing tissue oxidative stress levels through modulating SOD, GSH-PX, and CAT activities. Furthermore, the combined protocol further decreased the liver morphological score compared with solo treatment. Discussion and conclusion: Overall, the results indicate that the H. scoparia flavonoid-enriched fraction could be a promising candidate for future application as a pharmacological preconditioning agent against hepatic IRI.

Short-term inhalation of isoflurane improves the outcomes of intraportal hepatocyte transplantation

“…6B). Corroborating this finding, the serum levels of AST, ALT, and LDH, which are strongly associated with the damage of hepatocytes [46][47][48] , were also significantly suppressed in the ISO group in comparison to the AW group (Fig. 2A-C).…”

A systematic review on Hammada scoparia medicinal plant: Phytochemicals, traditional uses and biological activities

“…Hammada scoparia is widely used in traditional medicine in North Africa (Table 2) to prevent many diseases, such as hepatitis, inflammation, and obesity (Ezzeddine et al, 2016). Several traditional applications have revealed that extracts of Hammada scoparia have anti-cancer and anti-diabetic properties (Bouaziz et al, 2016;Boulanouar et al, 2013;Hamza et al, 2019;Saidi et al, 2015;Taïbi et al, 2020). This species commonly treats diseases, such as decoction, infusion, or cataplasm.…”