Natural killer cell biology: An update and future directions

Campbell, Kerry S.; Hasegawa, Jun

doi:10.1016/j.jaci.2013.07.006

Cited by 334 publications

(302 citation statements)

References 115 publications

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“…NK cells constitute 5-15% of lymphoid cells in human peripheral blood and are critical during immunity against intracellular infectious agents and tumors (18,19). NK cells have also immunoregulatory properties due to the secretion of multiple cytokines such as IFN-␥, TNF-␣, GM-CSF, and chemokines that contribute to modulate the innate immune response and to shift the adaptive immune response to a Th1-biased profile through interactions with DCs and macrophages (18, 20 -23).…”

mentioning

confidence: 99%

Expression and Functional Role of α7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells

Zanetti

Ziblat

Torres

et al. 2016

Journal of Biological Chemistry

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The homomeric ␣7 nicotinic receptor (nAChR) is one of the most abundant nAChRs in the central nervous system where it contributes to cognition, attention, and working memory. ␣7 nAChR is also present in lymphocytes, dendritic cells (DCs), and macrophages and it is emerging as an important drug target for intervention in inflammation and sepsis. Natural killer (NK) cells display cytotoxic activity against susceptible target cells and modulate innate and adaptive immune responses through their interaction with DCs. We here show that human NK cells also express ␣7 nAChR. ␣7 nAChR mRNA is detected by RT-PCR and cell surface expression of ␣7 nAChR is detected by confocal microscopy and flow cytometry using ␣-bungarotoxin, a specific antagonist. Both mRNA and protein levels increase during NK stimulation with cytokines (IL-12, IL-18, and IL-15). Exposure of cytokine-stimulated NK cells to PNU-282987, a specific ␣7 nAChR agonist, increases intracellular calcium concentration ([Ca2؉

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mentioning

confidence: 99%

Expression and Functional Role of α7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells

Zanetti

Ziblat

Torres

et al. 2016

Journal of Biological Chemistry

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“…NK cells display natural cytotoxicity against several tumors [20]. We determined the effect of PDO on NK cell cytotoxic function.…”

Section: Resultsmentioning

confidence: 99%

Safety and Efficacy of Polydioxanone Nano-Fibers as Anti-Inflammatory Agents

Gollapudi¹

2014

J Nanomedine Biotherapeutic Discov

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PDOPDO nano-fibers were a kind gift of Dongbang Acupuncture Ltd, Korea. Phagocytosis assayPhagocytosis was assessed by flow cytometry using pHrodo AbstractThe use of biodegradable polydioxanone (PDO) nano-fibers for various purposes is increasing but the effects of these fibers on immune responses are not well understood. We examined the potential stimulatory and inhibitory effects of PDO coated fibers on immune cell functions in vitro in humans and in vivo in mice. Short term in vitro exposure of human blood to PDO did not perturb the phagocytic function of human monocytes and neutrophils. In contrast lymphocytes exhibited an increased proliferative function in response to polyclonal T cell mitogen, PHA. However, cytokine secretion by monocytes and lymphocytes as well as NK cell cytotoxic effector cell functions were undisturbed by PDO exposure. Long term in vivo exposure to PDO had no effect on dendritic cells activation, cytokine secretion and T regulatory induction. Injection of PDO into mice with rheumatoid arthritis suggested that PDO nano-fibers tend to be anti-inflammatory as an increase in IL-10 was observed in the PDO treated groups. In spite of this the arthritic score and TNF-α and IFN-γ levels were not significantly different between PDO-treated and untreated rheumatoid arthritis induced mice. In conclusion, PDO nano-fibers have no significant adverse effect on immune functions and tend to induce anti-inflammatory responses upon long term exposure in vivo.

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“…(18,19). In addition, NK cells exhibit anti-tumor and anti-viral activity by releasing various types of cytokines, such as IFN-γ (20)(21)(22). Investigators have demonstrated that GPS promotes NK cell cytotoxicity and anti-tumor activity, but few studies have investigated the mechanisms underlying these effects (23,24).…”

Section: Effect Of Gps On the Mrna Expression Levels Of Ifn-γ In Thementioning

confidence: 99%

Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice

Sun

Guo

Feng

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the effects of Ginseng polysaccharides (GPS) on natural killer (NK) cell cytotoxicity in immunosuppressed mice. Cyclophosphamide (Cy) was used to construct an immunosuppressed mouse model. The mice in each group were submitted to gavages with 200 or 400 mg/kg GPS every day for 10 days. Magnetic-activated cell sorting was used to isolate spleen NK cells, and the NK cell cytotoxicity, blood distribution, expression levels of perforin and granzyme, and the mRNA expression levels of interferon (IFN)-γ were detected. Compared with the normal control group, the cytotoxicity and proportion of NK cells in the blood, and the expression levels of perforin, granzyme and IFN-γ mRNA in the Cy model group were significantly reduced (P<0.05). In addition, compared with the Cy model group, the cytotoxicity and proportion of NK cells in the whole blood, and the expression levels of perforin and granzyme in the NK cells in the Cy + low-dose GPS and Cy + high-dose GPS groups were significantly increased (P<0.05). However, the mRNA expression levels of IFN-γ in the NK cells did not significantly change (P>0.05). Compared with the normal control group, the cytotoxicity and proportion of NK cells in the whole blood, and the expression levels of perforin in the Cy + low-dose GPS and the Cy + high-dose GPS groups were significantly lower (P<0.05).However, the expression levels of granzyme in the NK cells was not significantly different, as compared with the normal control group (P>0.05). These results suggested that GPS promotes NK cell cytotoxicity in immunosuppressed mice by increasing the number of NK cells in the whole blood and upregulating the expression of perforin and granzyme. Thus, the present study investigated the molecular mechanism underlying NK cell activation by GPS, the research showed that GPS have a wide application prospects in the treatment of cancer and immunodeficiency diseases.

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Natural killer cell biology: An update and future directions

Cited by 334 publications

References 115 publications

Expression and Functional Role of α7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells

Expression and Functional Role of α7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells

Safety and Efficacy of Polydioxanone Nano-Fibers as Anti-Inflammatory Agents

Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice

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