Natural antibodies to IFN-gamma in man and their increase during viral infection.

Abstract

Natural antibodies to IFN-gamma were found in healthy individuals ranging from newborn babies to adults and, at higher levels, in patients suffering from different viral infections. During a viral infection, the titer of anti-IFN-gamma antibodies was observed to be correlated with the stage of the disease. Antibodies specific to IFN-gamma were affinity purified both from sera taken from healthy individuals and sera from viral-infected patients, by using a rIFN-gamma-coupled CNBr-activated Sepharose 4B column. The antibodies were found to be of the IgG class, and maintained their ability to bind rIFN-gamma. They were then tested for neutralizing activity and none of the IgG preparations we analyzed impaired the antiviral activity of rIFN-gamma. This finding suggests that the antigenic determinants recognized by these antibodies on the IFN-gamma molecule are located outside the site, on the IFN-gamma molecule, responsible for its antiviral activity.

Transient anti-cytokine autoantibodies superimpose the hyperinflammatory response in Kawasaki disease and multisystem inflammatory syndrome in children: a comparative cohort study on correlates of disease

“…11 , 12 , 13 Conversely, our data are in line with previous data describing transiently increased anti-IFN-y in patients suffering from different viral infections. 22 , 23 …”

Transient autoantibodies to danger signals

“…Thus, transient autoantibodies directed against danger signals could be an evolutionarily selected characteristic. Indeed, binding (non-neutralizing) anti-IFN-γ autoantibodies that wax and wane in response to viral disease have been observed since 1990 ( 15 ) and transient anti-HMGB1 autoantibodies emerge in some patients with septic shock and are highly predictive of survival ( 16 ). In patients with systemic lupus erythematosus, neutralizing anti-IFN-α autoantibodies were found to have positive effects akin to those of therapeutic anti-IFN monoclonals ( 17 ).…”

Anticorps anti-cytokines : quel impact clinique en pathologie humaine ?

“…Il existe une variation des taux sériques de certains ACA au cours d'épisodes infectieux. C'est le cas par exemple des anticorps anti-IFN-non neutralisants, dont le titre augmente lors d'une infection virale puis diminue après la résolution de l'infection [19]. Dans une étude Scandinave, les anti-IFN-étaient retrouvés dans les prépa-rations d'IgIV issues de dons réalisés en hiver, avec des taux plus élevés en présence d'anticorps anti-viraux, et n'étaient pas retrou-vés dans des préparations issues de dons réalisés en été [20].…”