Nanozymes with reductase-like activities: antioxidant properties and electrochemical behavior

Stasyuk, Nataliya; Gayda, Galina; Kavetskyy, Taras; Gonchar, Mykhailo

doi:10.1039/d1ra08127f

Cited by 4 publications

(6 citation statements)

References 55 publications

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“…The synthesized compounds were screened on their ability to oxidize o -DZ in the presence of H 2 O 2 by monitoring optical density at 525 nm. 38 It was shown that several synthesized NZs, especially nPtCu and nCu revealed the highest PO-like activities among all others ( Table 2 ).…”

Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

“…Nanozymes (NZs) were synthesized by the chemical reduction of metal ions from appropriate salts using CTAB and ascorbic acid (AA) according to the schemes which were reported by us recently with some modications. 38 The conditions of synthesis, which were chosen experimentally, are presented in Table ESI 1. † Briey: mixture 1, containing solutions of salt #1 and CTAB, was vigorously stirred for 5 min at 20 C and aer AA adding, it was heated at 100 C during 10 min.…”

Section: Synthesis Of Nanozymes By the Chemical Reduction Methodsmentioning

confidence: 99%

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Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages

et al. 2022

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Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Synthesis Of Nanozymes By the Chemical Reduction Methodsmentioning

confidence: 99%

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Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages

et al. 2022

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“…Then, solutions were mixed and supplemented with NaOH. PtZnNPs (further—PtZn) were synthesized from 10 mM H 2 PtCl 6 using the Zn bath deposition method [ 42 ] with our modification [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

et al. 2023

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L-Lactate is an indicator of food quality, so its monitoring is essential. Enzymes of L-Lactate metabolism are promising tools for this aim. We describe here some highly sensitive biosensors for L-Lactate determination which were developed using flavocytochrome b2 (Fcb2) as a bio-recognition element, and electroactive nanoparticles (NPs) for enzyme immobilization. The enzyme was isolated from cells of the thermotolerant yeast Ogataea polymorpha. The possibility of direct electron transfer from the reduced form of Fcb2 to graphite electrodes has been confirmed, and the amplification of the electrochemical communication between the immobilized Fcb2 and the electrode surface was demonstrated to be achieved using redox nanomediators, both bound and freely diffusing. The fabricated biosensors exhibited high sensitivity (up to 1436 A·M−1·m−2), fast responses, and low limits of detection. One of the most effective biosensors, which contained co-immobilized Fcb2 and the hexacyanoferrate of gold, having a sensitivity of 253 A·M−1·m−2 without freely diffusing redox mediators, was used for L-Lactate analysis in samples of yogurts. A high correlation was observed between the values of analyte content determined using the biosensor and referenced enzymatic-chemical photometric methods. The developed biosensors based on Fcb2-mediated electroactive nanoparticles can be promising for applications in laboratories of food control.

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“…GNZs further possess reductase activity towards substrates like p -nitrophenol [ 40 ]. This activity is induced by sodium borohydride, and produces p -aminophenol and changes the color from yellow to colorless [ 41 ].…”

Section: Mechanism Of Catalytic Activitymentioning

confidence: 99%

Surface modification strategies and the functional mechanisms of gold nanozyme in biosensing and bioassay

Garehbaghi

Ashrafi

Adam

et al. 2023

Materials Today Bio

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Nanozymes with reductase-like activities: antioxidant properties and electrochemical behavior

Abstract: Novel Zn/Cd/Cubd nanozymes possesses the ability to mimic coenzyme-dependent selenite reductase. A new amperometric biosensor for determination of selenite was constructed.

Cited by 4 publications

References 55 publications

Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages

Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

Surface modification strategies and the functional mechanisms of gold nanozyme in biosensing and bioassay

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