Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages

Stasyuk, Nataliya; Demkiv, Olha; Gayda, Galina; Zakalska, Oksana; Zakalskiy, Andriy; Serkiz, R.; Kavetskyy, Taras; Gonchar, Mykhailo

doi:10.1039/d2ra02106d

Cited by 3 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is worth mentioning that the electroactive NPs described in our work are artificial peroxidases (POs) or PO-like nanozymes. It was demonstrated by us earlier [ 19 , 24 , 44 , 45 , 46 , 47 , 48 ], and in this work (data not shown) that all NPs used here possess pseudo-PO activity that makes them able to decompose H 2 O 2 both in solution and on amperometric electrodes.…”

Section: Discussionsupporting

confidence: 58%

“…Bi-and trimetallic NPs were synthesized by the chemical reduction of metal ions from appropriate salts [ 46 , 47 ] or by the chemical bath deposition method [ 48 ]. PtRu/NPs, NiPtPd/NPs, and CuCo/NPs (further—PtRu, NiPtPd, and CuCo, respectively) were obtained and characterized as described in our recent works ([ 46 , 47 , 48 ], respectively).…”

Section: Methodsmentioning

confidence: 99%

“…All the synthesized NPs were collected by centrifugation, washed with water, and stored as a water suspension at 4 °C until use [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

“…The analytical parameters of the electrodes were estimated and statistically processed as described earlier [ 44 , 45 , 46 , 47 ]. Morphological analyses of the NPs by scanning electron microscopy (SEM) were performed as described earlier [ 44 , 45 , 46 , 47 , 48 ].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay

et al. 2022

Self Cite

View full text Add to dashboard Cite

Laccase is a copper-containing enzyme that does not require hydrogen peroxide as a co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction of enzyme-based biosensors. Metals, metal oxides, semiconductors, and composite NPs perform various functions in electrochemical transformation schemes as a platform for the enzyme immobilization, a mediator of an electron transfer, and a signal amplifier. We describe here the development of amperometric biosensors (ABSs) based on laccase and redox-active micro/nanoparticles (hereafter—NPs), which were immobilized on a graphite electrode (GE). For this purpose, we isolated a highly purified enzyme from the fungus Trametes zonatus, and then synthesized bi- and trimetallic NPs of noble and transition metals, as well as hexacyanoferrates (HCF) of noble metals; these were layered onto the surfaces of GEs. The electroactivity of many of the NPs immobilized on the GEs was characterized by cyclic voltammetry (CV) experiments. The most effective mediators of electron transfer were selected as the platform for the development of laccase-based ABSs. As a result, a number of catechol-sensitive ABSs were constructed and characterized. The laccase/CuCo/GE was demonstrated to possess the highest sensitivity to catechol (4523 A·M−1·m−2) among the tested ABSs. The proposed ABSs may be promising for the analysis of phenolic derivatives in real samples of drinking water, wastewater, and food products.

show abstract