Nanozyme‐Engineered Bioglass through Supercharged Interface for Enhanced Anti‐Infection and Fibroblast Regulation

Abstract

Effective therapy of infection impaired tissue defects has long been an important but challenging topic, and alternative antibiotic‐free solutions are greatly demanded to tackle bacterial infections and promote tissue repair. Herein, the use of supercharged gold nanoclusters (AuNCs) with enhanced enzyme mimic activity as novel interface modulator of bioactive glass nanoparticles (BGN) for infected wound treatment is reported. The supercharged AuNCs exhibit extraordinary affinity toward BGN, leading to the robust immobilization of AuNCs on BGN (BGN‐AuNCs). Functional AuNCs endow BGN with superior peroxidase‐like activity for catalytic antibacterial action, which can kill bacteria with a concentration down to 75 µg mL−1 within 6 h due to the production of highly toxic ·OH. Moreover, expression level of COL‐1, TGF‐β, and FGF‐2 in fibroblasts is actively up‐regulated to 2.9, 3.1, and 1.6 times higher than that of control group due to the positive effect of BGN‐AuNCs on cell proliferation. Meanwhile, intrinsic photoluminescent property of AuNCs enables the direct visualization of BGN at both in vitro and in vivo levels for potentially bioimaging‐guided therapy. Further in vivo results demonstrate that BGN‐AuNCs hold enormous promise for infectious disease therapy and pro‐regeneration, and the proposed supercharged interface engineering strategy is attractive for developing advanced functional nanomedicines.

Coordination‐Driven One‐Step Rapid Self‐Assembly Synthesis of Dual‐Functional Ag@Pt Nanozyme

“…Consistent with previous research, the experimental results show that the antibacterial effect of nanomaterials on G‐ is greater than that of G+ due to the different cell wall compositions and structures of both. [ 46,47 ] At the same time, the H 2 O 2 treatment group did not affect the BE, proving that the antibacterial mechanism of Ag@Pt nanozymes primarily realized the POD‐like activity of the nanozymes catalyzing H 2 O 2 to generate ROS. As can be seen from Figure S20 (Supporting Information), ROS generated by NA‐Ag@Pt nanozyme is higher than that by FNA‐Ag@Pt nanozyme in the bacterium, and E. coil is more sensitive to nanozymes compared with SA .…”

Nanomaterials‐Enabled Physicochemical Antibacterial Therapeutics: Toward the Antibiotic‐Free Disinfections

“…[ 132,133 ] Because ROS simultaneously oxidize diverse cellular substances (e.g., nucleic acids, proteins, and lipids) crucial for proper cell function, [ 134 ] nanozymes eliminate bacteria and may delay the onset of bacterial resistance (Figure 4e). [ 19,135–138 ]…”

“…[132,133] Because ROS simultaneously oxidize diverse cellular substances (e.g., nucleic acids, proteins, and lipids) crucial for proper cell function, [134] nanozymes elim-inate bacteria and may delay the onset of bacterial resistance (Figure 4e). [19,[135][136][137][138] As a representative example of ROS generation nanocatalyst, a zeolitic imidazolate framework (ZIF)−8-derived Zn singleatom catalyst with a strong peroxidase (POD)-mimetic property showed excellent antibacterial activities (Figure 4f,g). [33] This single-atom nanocatalyst can inhibit the growth of P. aeruginosa at the rate of 99.87 % in vitro and promote the healing of P. aeruginosa-infected wounds in vivo (Figure 4h).…”

Bioceramic materials with ion‐mediated multifunctionality for wound healing

“…For bioceramic materials, enhanced wound healing ability and anti-infection function can be achieved by tailoring the chemical compositions to incorporating specific inorganic elements with intrinsic antibacterial effects such as Ag, Au, Cu, Zn, Mo, Ga, Ce ions. 25,[111][112][113][114][115][116] -containing hydroxyapatite (HCA)-coated BBG (nano-HCA@BG) and (ii) evaluation of the wound healing effect of nano-HCA@BG powders in mouse skin defects. Reproduced with permission.…”

“…However, such pH‐dependent antibacterial activity is limited for an effective anti‐infection functionality. For bioceramic materials, enhanced wound healing ability and anti‐infection function can be achieved by tailoring the chemical compositions to incorporating specific inorganic elements with intrinsic antibacterial effects such as Ag, Au, Cu, Zn, Mo, Ga, Ce ions 25,111–116 . For example, Li et al.…”