Nanowires array modified electrode for enhanced electrochemical detection of nucleic acid

Ramulu, T.S.; Reddy, Venu; Sinha, Brajalal; Lim, Byeonghwa; Jeon, Seong Gyu; Yoon, Seok Soo; Kim, C.G.

doi:10.1016/j.bios.2012.07.034

Cited by 48 publications

(19 citation statements)

References 41 publications

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“…6(a–d) . In brief, the spotted NF bioelectrode has good linearity, and a detection limit of 100 fM was estimated using a signal-to-noise ratio of more than 3σ 47 48 . The Rct value of the t-DNA (1 nM) was ∼6.7 MΩ, which was nearly 8.5 times larger than that of single-base-mismatched DNA (∼0.78 MΩ) at the same concentration, indicating that the DNA biosensor has excellent sequence specificity towards even a single base mismatch.…”

Section: Resultsmentioning

confidence: 99%

‘Spotted Nanoflowers’: Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture

Perumal

Hashim

Gopinath

et al. 2015

Sci Rep

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Hybrid gold nanostructures seeded into nanotextured zinc oxide (ZnO) nanoflowers (NFs) were created for novel biosensing applications. The selected ‘spotted NFs’ had a 30-nm-thick gold nanoparticle (AuNP) layer, chosen from a range of AuNP thicknesses, sputtered onto the surface. The generated nanohybrids, characterized by morphological, physical and structural analyses, were uniformly AuNP-seeded onto the ZnO NFs with an average length of 2–3 μm. Selective capture of molecular probes onto the seeded AuNPs was evidence for the specific interaction with DNA from pathogenic Leptospirosis-causing strains via hybridization and mis-match analyses. The attained detection limit was 100 fM as determined via impedance spectroscopy. High levels of stability, reproducibility and regeneration of the sensor were obtained. Selective DNA immobilization and hybridization were confirmed by nitrogen and phosphorus peaks in an X-ray photoelectron spectroscopy analysis. The created nanostructure hybrids illuminate the mechanism of generating multiple-target, high-performance detection on a single NF platform, which opens a new avenue for array-based medical diagnostics.

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Section: Resultsmentioning

confidence: 99%

‘Spotted Nanoflowers’: Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture

Perumal

Hashim

Gopinath

et al. 2015

Sci Rep

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“…This means that detection limits of such devices are two to three orders of magnitude better than that of conventional macroelectrodes [ 22 ]. The reaction rate can further be enhanced in the nanowire electrodes since a vertically aligned nanowire can work as an “electron antennae” and enhance the electron transfer for the reactions [ 23 , 24 ].…”

Section: Resultsmentioning

confidence: 99%

Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array

et al. 2016

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In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.

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“…Moreover, application Özt€ urk et al [194] Fig . 20 ZnO nanowires obtained by DC electrodeposition into anodic alumina template (Reprinted from Özt€ urk et al [194] Handbook of Nanoelectrochemistry DOI 10.1007/978-3-319-15207-3_19-1 # Springer International Publishing Switzerland 2015 of various nanofabrication techniques, including electrochemical deposition and electropolymerization, allows to form Ag [122], Au [212,265], and polypyrrole [151,250,302] nanowire-based sensors. Also nanotubes fabricated with anodic alumina template employing techniques are being applied as sensors [299,301].…”

Section: Applications Of Anodic Aluminum Oxidementioning

confidence: 99%

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

Stępniowski

Bojar

2015

Handbook of Nanoelectrochemistry

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Anodic aluminum oxide (AAO) consists of parallel pores arranged in a honeycomb-like array. Since a two-step self-organized approach in anodic alumina fabrication was invented, tremendous attention was paid to this material. Structural features of anodic alumina, like pore diameter, interpore distance, and nanoporous oxide thickness, can be fully controlled by operating conditions, including type, concentration and temperature of the electrolyte, applied voltage, and duration of the anodization process. Moreover, these operating conditions have also a major impact on the nanoporous array arrangement. Quantitative arrangement analysis methods employing fast Fourier transforms have been developed to assess the nanopores' arrangement. The most frequent application of the anodic aluminum oxide is a template-assisted fabrication of nanostructures. Templates made of the AAO are being successfully applied in fabrication of nanowires, nanotubes, and nanodots by using diversity of techniques, including electrochemical deposition, physical and chemical vapor deposition, sol-gel techniques, etc. Due to the size of obtained nanostructures, magnetic, electric, piezoelectric, luminescent, and catalytic properties of the deposited materials can be significantly improved. Thus, control of the nanopores' structural features and arrangement is crucial for AAO applications in nanotechnology.

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Nanowires array modified electrode for enhanced electrochemical detection of nucleic acid

Cited by 48 publications

References 41 publications

‘Spotted Nanoflowers’: Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture

‘Spotted Nanoflowers’: Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture

Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

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