Nanovesicle encapsulation of antimicrobial peptide P34: physicochemical characterization and mode of action on Listeria monocytogenes

Malheiros, Patrícia da Silva; Sant’Anna, Voltaire; Micheletto, Yasmine Miguel Serafini; Silveira, Nádya Pesce da; Brandelli, Adriano

doi:10.1007/s11051-011-0278-2

Cited by 50 publications

(18 citation statements)

References 35 publications

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“…In addition, the presence of the whey peptides within the liposomes would be expected to increase the electrostatic repulsion between them and thereby increase their aggregation stability. This is contrary to previously reported decreases in surface charge magnitude when peptides were loaded in liposomes (Mosquera et al, 2016;da Silva Malheiros et al, 2011). As shown in Table 1, the ζ-potential of liposomes containing the high molecular weight peptides is significantly lower in magnitude compared to those of liposomes loaded with smaller peptides.…”

Section: Surface Chargecontrasting

confidence: 99%

“…Moreover, liposomes containing the higher molecular weight peptide fractions had lower PDI values indicating they formed more uniform colloidal dispersions. The liposomes loaded with the whey peptide fractions in this study are less uniformly dispersed than those reported previously in the literature (da Rosa Zaverze et al, 2014;Mosquera et al, 2014;da Silva Malheiros et al, 2011). It was observed that the peptide heterogeneity, based on the number of peptides within the fractions, affects the uniformity of the liposome dispersion (r s =0.7).…”

Section: Polydispersity Index (Pdi)contrasting

confidence: 45%

“…High positive or negative surface charges result in strong electrostatic repulsive interactions between liposomes, which prevents them from coming into close proximity and fusing together (da Silva Malheiros, Sant'Anna, Micheletto, da Silveira, & Brandelli, 2011). A surface charge of ±30 mV is typically taken to be sufficient to ensure the formation of a stable aqueous dispersion, due to electrostatic stabilization.…”

Section: Surface Chargementioning

confidence: 99%

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Encapsulation of bioactive whey peptides in soy lecithin-derived nanoliposomes: Influence of peptide molecular weight

2016

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Section: Surface Chargecontrasting

confidence: 99%

Section: Polydispersity Index (Pdi)contrasting

confidence: 45%

Section: Surface Chargementioning

confidence: 99%

See 1 more Smart Citation

Encapsulation of bioactive whey peptides in soy lecithin-derived nanoliposomes: Influence of peptide molecular weight

2016

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“…A similar observation was reported by Mosquera et al (), who found that peptides decrease the surface charge of liposomes when loaded into liposomes. In contrast to these results, Da Silva Malheiros, Sant'Anna, Micheletto, Da Silveria, and Brandelli () reported that loading peptides significantly increased the liposome surface charge; values more positive or negative than +30 mV or −30 mV are normally considered stable (McClements, ).…”

Section: Resultsmentioning

confidence: 86%

Functionality of porcine skin hydrolysates produced by hydrothermal processing for liposomal delivery system

Choi

Min

2017

J Food Biochem

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Porcine skin proteins were hydrolyzed using hydrothermal processing (HTP) and fractionated by membrane ultrafiltration (UF) into peptides with different molecular weights (1-10 kDa). The porcine skin hydrolysates (PSHs) were analyzed for their antioxidant, antiaging, and skin permeation properties. Additionally, the PSHs were incorporated into liposomes with different charges to maintain and/or enhance their beneficial effect. The results showed that both HTP and UF-II (1-3 kDa) had significantly high antioxidant and antiaging effects and good skin permeability. Based on the results for PSH-loaded liposomes, the best carrier for incorporating PSHs was a positively charged liposome that had been prepared using positively charged lipid. PSH loaded with positively charged liposomes (PSH/LIP-P) had the following droplet properties: 71.11 nm, 0.208 PDI (polydispersity index), and 38.37 mV. PSH/LIP-P also had an incorporation efficiency of 70.72%, and the liposomes maintained their physical droplet stability during storage. Practical applicationsThe findings of this study highlighted porcine skin hydrolysates (PSH) produced by hydrothermal processing and membrane ultrafiltration with high antioxidant and antiaging effects and good skin permeability. PSH-loaded liposome capsules maintained a small size and high incorporation efficiency for PSH. The PSHs and PSH-loaded liposomes could be tested further for their in vivo functionality as nutraceutical or cosmeceutical ingredients. Hence, the finding could be useful information for developing potential commercial products in the food, cosmetics, and related industries. K E Y W O R D Santiaging effect, antioxidant effect, charged liposome, incorporation, porcine skin hydrolysate | I NTR OD U CTI ONThe functional food and cosmetic markets have grown rapidly over the last decade and are associated with consumer awareness of their roles in preventing disease and promoting health (Khiari, Ndagijimana, & Betti, 2014). Collagen has been widely used in food, cosmetics, pharmaceuticals, and medical applications due to its biological and functional properties (Li, Jia, & Yao, 2009;Zeng, Zahng, Adhikari, & Mujumdar, 2013). It is a fibrous insoluble protein that is mostly found in skin, cartilage, and connective tissues; it constitutes up to 30% of all body proteins and is the most abundant animal protein (Johnston, 1990). Recently, there has been interest in collagen with novel biologi- Each approach has advantages and limitations (McClements, 2014(McClements, , 2015. Liposomes are particularly suitable carriers for peptide delivery because they can encapsulate polar, nonpolar, and amphiphilic acids from protein hydrolysates (Mohan et al., 2016). In addition, liposomes can be easily produced from food grade materials; they can trap and release hydrophilic and hydrophobic ingredients and facilitate targeted actions (Jeon, Yoo, & Park, 2015).Therefore, the objective of this study was to produce porcine skin hydrolysates (PSHs) and investigate their antioxidant activity and phy...

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“…A high value of zeta potential indicates strong repulsive interactions between anionic groups on the surfaces of liposomes which prevent their aggregation. [44] A zeta potential value of ≥±30 mV indicates a good electrostatic stability of nanocapsules suspension. Similarly, nanoliposomes with low electrostatic stability of <−30 mV, [21,45] and those with high electrostatic stability of >−30 mV [24,25] have been reported.…”

Section: Physicochemical Stability Of the Nanoliposomesmentioning

confidence: 99%

Comparative physicochemical stability and efficacy study of lipoid S75-biopeptides nanoliposome composite produced by conventional and direct heating methods

Auwal

Zarei

Tan

et al. 2018

International Journal of Food Properties

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Liposomes are used as effective nanodelivery devices to improve the physicochemical stability and biological efficacy of the encapsulated peptides and proteins. In this study, nanoliposome composite of lipoid S75entrapped angiotensin I-converting enzyme (ACE)-inhibitory biopeptides was prepared by conventional (BLS75-CM) and direct heating (BLS75-DHM) methods. The nanoliposomes (BLS75-CM and BLS75-DHM) were stored at 4°C for 8 weeks and evaluated for physicochemical stability in terms of particle size, polydispersity index (pdi), zeta potential, and encapsulation efficiency (EE). These were also studied for residual ACE-inhibitory efficacy following their digestion under simulated gastrointestinal tract condition. The BLS75-CM was found to maintain higher physicochemical stability in terms of particle size, pdi, and zeta potential compared to BLS75-DHM. However, the BLS75-DHM indicated higher EE and efficacy with greater residual ACE-inhibitory activity of 47.37% compared to 44.18% and 36.84% that were obtained for the digested BLS75-CM and digested biopeptides without encapsulation, respectively. In vitro release study showed a cumulative biopeptides release of 66.41% and 69.00% from BLS75-CM and BLS75-DHM, respectively. The results of transmission electron microscopy showed spherical appearance of the nanoliposome capsules while Fourier Transform Infrared spectroscopy indicated the presence of ionic complexation and hydrogen bonds between the biopeptides and their phospholipid matrix. ARTICLE HISTORY Highlights• Nanoliposomes improved the physiochemical stability of the encapsulated biopeptides. • Nanoliposomes formed by CM showed higher physical stability. • Nanoliposomes prepared by direct heating method exerted higher ACE-inhibitory efficacy.

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Nanovesicle encapsulation of antimicrobial peptide P34: physicochemical characterization and mode of action on Listeria monocytogenes

Cited by 50 publications

References 35 publications

Encapsulation of bioactive whey peptides in soy lecithin-derived nanoliposomes: Influence of peptide molecular weight

Encapsulation of bioactive whey peptides in soy lecithin-derived nanoliposomes: Influence of peptide molecular weight

Functionality of porcine skin hydrolysates produced by hydrothermal processing for liposomal delivery system

Comparative physicochemical stability and efficacy study of lipoid S75-biopeptides nanoliposome composite produced by conventional and direct heating methods

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