Comparative physicochemical stability and efficacy study of lipoid S75-biopeptides nanoliposome composite produced by conventional and direct heating methods

Abstract

Liposomes are used as effective nanodelivery devices to improve the physicochemical stability and biological efficacy of the encapsulated peptides and proteins. In this study, nanoliposome composite of lipoid S75entrapped angiotensin I-converting enzyme (ACE)-inhibitory biopeptides was prepared by conventional (BLS75-CM) and direct heating (BLS75-DHM) methods. The nanoliposomes (BLS75-CM and BLS75-DHM) were stored at 4°C for 8 weeks and evaluated for physicochemical stability in terms of particle size, polydispersity index (pdi), zeta potential, and encapsulation efficiency (EE). These were also studied for residual ACE-inhibitory efficacy following their digestion under simulated gastrointestinal tract condition. The BLS75-CM was found to maintain higher physicochemical stability in terms of particle size, pdi, and zeta potential compared to BLS75-DHM. However, the BLS75-DHM indicated higher EE and efficacy with greater residual ACE-inhibitory activity of 47.37% compared to 44.18% and 36.84% that were obtained for the digested BLS75-CM and digested biopeptides without encapsulation, respectively. In vitro release study showed a cumulative biopeptides release of 66.41% and 69.00% from BLS75-CM and BLS75-DHM, respectively. The results of transmission electron microscopy showed spherical appearance of the nanoliposome capsules while Fourier Transform Infrared spectroscopy indicated the presence of ionic complexation and hydrogen bonds between the biopeptides and their phospholipid matrix. ARTICLE HISTORY Highlights• Nanoliposomes improved the physiochemical stability of the encapsulated biopeptides. • Nanoliposomes formed by CM showed higher physical stability. • Nanoliposomes prepared by direct heating method exerted higher ACE-inhibitory efficacy.

Effect of hydrolysis time and peptide content on dpph* radical scavenging activity of bromelain- and papain-generated hydrolysates from defatted cocoa beans (Theobroma cacao l.) powder

“…A 200 µL each of bromelain-and papain-generated DCB protein hydrolysates were separately mixed with 200 µL of 20% (w/v) TCA solution and allowed to stand for 30 min. The precipitate formed was centrifuged at 7800 x g for 12 min and the TCA-soluble peptide content of the resulting supernatant was determined using BCA protein assay kit method as previously reported (Auwal et al, 2018a;Auwal et al, 2018b) with bovine serum albumin (BSA) as standard. The TCA-soluble peptide yield was calculated as follows: TCA -soluble peptide (%) = X/Y x 100% where X: peptide content of the supernatant and Y: total protein content of the sample before enzymatic hydrolysis.…”

Improving Health-Promoting Effects of Food-Derived Bioactive Peptides through Rational Design and Oral Delivery Strategies

“…Besides, results of the in vivo efficacy of these chitosan nanoparticles in SHRs indicated a dose-dependent blood pressure lowering effect that was significantly higher than that caused by the non-encapsulated peptides [111]. In addition, the encapsulation of stone fish-derived bioactive peptides in nanoliposomes improved peptide stability against in vitro gastrointestinal digestion, although no data about the antihypertensive effect were provided [112]. Similarly, improved stability and bioavailability of nanoliposomes loaded with an ACE inhibitory peanut peptide fraction in a simulated gastrointestinal tract were reported [108].…”