Nanotopography/Mechanical Induction of Stem-Cell Differentiation

Teo, Benjamin Kim Kiat; Ankam, Soneela; Chan, Lesley Y.; Yim, Evelyn K.F.

doi:10.1016/s0091-679x(10)98011-4

Cited by 67 publications

(52 citation statements)

References 176 publications

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“…When human mesenchymal stem cells were cultured on polydimethylsiloxane with nanogratings of 250 nm lines, mesenchymal stem cells developed aligned stress fibers, smaller focal adhesions and a dense actin cytoskeleton. Expression of different integrin sub units were down regulated in these cells on nanogratings [17,18]. There is convincing evidence that changes in structural organization and expression of adhesion related cellular components due to a structured material surface commit the direction of stem cell differentiation.…”

Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 91%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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Abstract:The interaction of cells with material surfaces is of fundamental relevance and contributes to the clinical success of implants. Cells sense their physico-chemical environment resulting in focal adhesion reorganization, spatio-temporal localization and activation of integrin dependent signalling proteins as well as phenotypic changes, e.g., of the actin cytoskeleton. The technology of designing instructive biomaterials involves chemical modifications by grafting of chemical groups, adhesion ligands and growth factors. Physical modifications of the materials are created by structuring the surfaces stochastically and geometrically and by modifications of the material stiffness. Insights into the mechanism at the interface that are involved in the regulation of cells such as stem cells, osteoblasts and chondrocytes by materials will advance the development of innovative biomaterials in regenerative medicine.

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Section: Cell Regulation By Physical Characteristics Of a Materials Tomentioning

confidence: 91%

Cell-material interaction

Rychly

Nebe

2013

BioNanoMaterials

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“…This activation was interpreted as a sign of healthy adhesion; the authors also noted the importance of FAK activation as a tool for interpreting extracellular signals (Marino et al 2010). Importantly, FAK phosphorylation at tryosine 397 has previously been shown to play a role in mechanically stimulated differentiation (Teo et al 2010;Rubin et al 2006). This hypothesis of substrateinduced ASC osteogenesis is illustrated in Fig.…”

Section: Hypotheses Of Mechanismmentioning

confidence: 88%

“…Two proposed mechanisms for scaffoldinduced osteogenesis: (1) altered behavior of focal adhesions, a mechanism relying on direct connections between ECM and nucleus through FAK activation; and (2) an indirect mechanism, stimulation by scaffold ions entering through, e.g., a calcium receptor poly(glycolic acid) (PGA), have predictable and reproducible properties and can be less brittle than ceramics Burg and Kellam 2000). Another advantage is that polymers may be delicately shaped to present cues for osteogenesis on the nanometer scale (Reed et al 2009;Sefcik et al 2008;Teo et al 2010). But, polymeric materials may present biocompatibility problems (Hao et al 2010).…”

Section: Substrate Stimulusmentioning

confidence: 99%

“…ECM also presents chemical cues, because a lot of growth factors are stored in ECM. Additionally, nanoscale topographical features in growth substrates influence stem cell behavior (Teo et al 2010). Finally, elasticity has a plausible effect on osteogenesis of ASC (Hanson et al 2009;Engler et al 2006;McCullen et al 2010b;Joshi and Webb 2008).…”

Section: Hypotheses Of Mechanismmentioning

confidence: 99%

“…To summarize studies supporting development of vasculogenic/ osteogenic grafts generated from adipose-derived cells Teo et al (2010) Nanotopography/mechanical induction of stem cell differentiation…”

Section: Introductionmentioning

confidence: 99%

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Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

Salazar

Onodera

2012

Biophys Rev

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Developing bone is subject to the control of a broad variety of influences in vivo. For bone repair applications, in vitro osteogenic assays are routinely used to test the responses of bone-forming cells to drugs, hormones, and biomaterials. Results of these assays are used to predict the behavior of bone-forming cells in vivo. Stem cell research has shown promise for enhancing bone repair. In vitro osteogenic assays to test the bone-forming response of stem cells typically use chemical solutions. Stem cell in vitro osteogenic assays often neglect important biophysical cues, such as the forces associated with regular weight-bearing exercise, which promote bone formation. Incorporating more biophysical cues that promote bone formation would improve in vitro osteogenic assays for stem cells. Improved in vitro osteogenic stimulation opens opportunities for "preconditioning" cells to differentiate towards the desired lineage. In this review, we explore the role of select biophysical factors-growth surfaces, tensile strain, fluid flow and electromagnetic stimulation-in promoting osteogenic differentiation of stem cells from human adipose. Emphasis is placed on the potential for physical microenvironment manipulation to translate tissue engineering and stem cell research into widespread clinical usage.

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Stem‐Cell Responses to Surface Nanotopographies

Wang

Tsai

2015

Stem‐Cell Nanoengineering

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Nanotopography/Mechanical Induction of Stem-Cell Differentiation

Cited by 67 publications

References 176 publications

Cell-material interaction

Cell-material interaction

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

Stem‐Cell Responses to Surface Nanotopographies

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