Nanostructured interfaces with RGD arrays to control cell–matrix interaction

Huang, Jinghuan; Ding, Jiandong

doi:10.1039/b927168f

Cited by 49 publications

(35 citation statements)

References 95 publications

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“…Binding of several integrins to multivalent ligands has also been shown to result in integrin clustering in case the average ligand spacing is below 70 nm (Huang and Ding, 2010). Further, other researchers conducted cysteine disulfide bond scanning of full-length integrin subunits, suggesting that presence of multivalent ligand is sufficient for integrin clustering to occur and homo-oligomerization does not take place in vivo (Wang et al, 2011).…”

Section: Integrins Team Up (Integrin Clustering)mentioning

confidence: 96%

Mechanotransduction Pathways Linking the Extracellular Matrix to the Nucleus

Jahed

Shams

Mehrbod

et al. 2014

International Review of Cell and Molecular Biology

102

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Section: Integrins Team Up (Integrin Clustering)mentioning

confidence: 96%

Mechanotransduction Pathways Linking the Extracellular Matrix to the Nucleus

Jahed

Shams

Mehrbod

et al. 2014

International Review of Cell and Molecular Biology

102

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“…It is increasingly recognized that the spatial distribution of adhesive ligands plays a critical role on cell adhesion behavior [187,188]. How to precisely manipulate the distribution of these adhesive ligands on the non-fouling backgrounds using the LbL approaches remains to be a challenge.…”

Section: Multifunctional Lbl Coatings With Selective Adhesion and Nonmentioning

confidence: 99%

Controlling cell adhesion using layer-by-layer approaches for biomedical applications

Guo

Zhu

Loh

2017

Materials Science and Engineering: C

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“…Cell behavior on RGD-conjugated substrates depend on peptide densities [28][29]. It is reported that RGD nano-spacing is crucial to specific adhesion of cells [30][31] and the differentiation of stem cells [32].In order to understand and control surface-immobilized peptides for modulating cell behavior, it is important to minimize non-specific cell-substrate interactions. However, in conventional cell culture with serum-containing medium, non-specific adsorption of serum proteins to culture substrates is inevitable.…”

mentioning

confidence: 99%

Modulation of the stemness and osteogenic differentiation of human mesenchymal stem cells by controlling RGD concentrations of poly(carboxybetaine) hydrogel

Chien

Shih

et al. 2014

Biotechnology Journal

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In vitro modulation of the differentiation status of mesenchymal stem cells (MSCs) is important for their application to regenerative medicine. We suggested that the morphology and differentiation states of MSCs could be modulated by controlling the cell affinity of a substrate. The objective of this study was to investigate the effects of surface bio-adhesive signals on self-renewal and osteogenic differentiation of MSCs using a low-fouling platform. Cell-resistant poly(carboxybetaine) hydrogel was conjugated with 5 μM or 5 mM of cell-adhesive arginine-glycine-aspartic acid (RGD) peptides in order to control the cells' affinity to the substrate. Human mesenchymal stem cells (hMSCs) were cultured on the RGD-modified poly(carboxybetaine) hydrogel and then the cells' states of stemness and osteogenic differentiation were evaluated using reverse-transcriptase polymerase chain reaction. The hMSCs formed three-dimensional spheroids on the 5 μM RGD substrate, while cells on the 5 mM RGD substrate exhibited spreading morphology. Furthermore, cells on the 5 μM RGD hydrogel maintained a better stemness phenotype, while the hMSCs on the 5 mM RGD hydrogel proliferated faster and underwent osteogenic differentiation. In conclusion, the stemness of hMSCs was best maintained on a low RGD surface, while osteogenic differentiation of hMSCs was enhanced on a high RGD surface. monolayer culture [13][14], while MSCs with spheroid morphology tend to sustain pluripotent ability during extended culturing [14][15][16]. Cell adhesion to the extracellular matrix (ECM) in a native tissue is initiated by the binding of cell membrane receptors (e.g. integrins) to ECM adhesion proteins such as fibronectin and laminin [17]. Similarly, cell attachment to artificial substrates is usually mediated by surfacebound adhesion proteins. On the other hand, cell-cell attachment is mediated by other types of cell receptors such as cadherins [18]. We suggest that cell morphology during in vitro culture could be determined by the relative affinity between cell-substrate and cell-cell interactions. When the cell-substrate interaction is stronger than the cell-cell interaction, cells attach to and spread on the substrate. Conversely, when the cell-substrate interaction is weaker than the cell-cell interaction, cells tend to form spheroids on the surface [19].We suggested that by controlling the cell affinity of a substrate the morphology and differentiation status of MSCs could be modulated. To this end, surface conjugation of ECM adhesion proteins such as fibronectin, collagen, and laminin, is a common strategy. Alternatively, conjugation of peptides containing cell-binding domains of ECM adhesion proteins is another popular approach. The advantages of peptides compared to intact proteins include cost effectiveness and reduced vulnerability to denaturation. Among the peptides that mediate cell adhesion, arginine-glycine-aspartic acid (RGD), as found in fibronectin and laminin, is the most commonly applied cell-binding peptide for substrate modification in ...

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Nanostructured interfaces with RGD arrays to control cell–matrix interaction

Cited by 49 publications

References 95 publications

Mechanotransduction Pathways Linking the Extracellular Matrix to the Nucleus

Mechanotransduction Pathways Linking the Extracellular Matrix to the Nucleus

Controlling cell adhesion using layer-by-layer approaches for biomedical applications

Modulation of the stemness and osteogenic differentiation of human mesenchymal stem cells by controlling RGD concentrations of poly(carboxybetaine) hydrogel

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