Controlling cell adhesion using layer-by-layer approaches for biomedical applications

Guo, Shan; Zhu, Xiaoying; Loh, Xian Jun

doi:10.1016/j.msec.2016.03.074

Cited by 91 publications

(63 citation statements)

References 185 publications

(237 reference statements)

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“…This may be due to the roughness of the material surface, based on the SEM results. The water contact angles for LPH-0 and LPH-8 films were in the reference range (45 • -76 • ) with proper cells adhesion in biomedical applications [46].…”

Section: Swelling Properties Of Lph Filmsmentioning

confidence: 90%

“…This may be due to the roughness of the material surface, based on the SEM results. The water contact angles for LPH-0 and LPH-8 films were in the reference range(45°-76°) with proper cells adhesion in biomedical applications [46]. The water absorption measurement of LPH films is very important because the mechanism for hydrolytic degradation of the polyurethane polymer is hydrolysis of ester and urethane groups.…”

Section: Swelling Properties Of Lph Filmsmentioning

confidence: 99%

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Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

et al. 2019

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A nontoxic and biodegradable polyurethane was prepared, characterized, and evaluated for biomedical applications. Stretchable, biodegradable, and biocompatible polyurethanes (LPH) based on L-lysine diisocyanate (LDI) with poly(ethylene glycol) (PEG) and polyhydroxyalkanoates(PHA) of different molar ratios were synthesized. The chemical and physical characteristics of the LPH films are tunable, enabling the design of mechanically performance, hydrophilic, and biodegradable behavior. The LPH films have a Young’s modulus, tensile strength, and elongation at break in the range of 3.07–25.61 MPa, 1.01–9.49 MPa, and 102–998%, respectively. The LPH films demonstrate different responses to a change of temperature from 4 to 37 °C, with the swelling ratio for the same sample at equilibrium varying from 184% to 151%. In vitro degradation tests show the same LPH film has completely different degradation morphologies in pH of 3, 7.4, and 11 phosphate buffered solution (PBS). In vitro cell tests show feasibility that some of the LPH films are suitable for culturing rat bone marrow stem cells (rBMSCs), for future soft-tissue regeneration. The results demonstrate the feasibility of the LPH scaffolds for many biomedical applications.

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Section: Swelling Properties Of Lph Filmsmentioning

confidence: 90%

Section: Swelling Properties Of Lph Filmsmentioning

confidence: 99%

Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

et al. 2019

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“…The LbL technique has been used to produce coatings with antibacterial applications, with the capacity of releasing antibacterial agents, or capable of killing bacteria by contact, as well as surfaces combining the capacity of releasing of antibacterials with a low bacteria adhesion [10,11]. For biomedical applications, the control of mammalian and bacteria cells adhesion is a challenging task and of uppermost importance for the successful implementation of new material to be in contact with biological environments [12].…”

Section: Introductionmentioning

confidence: 99%

Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing: Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria

Muzzio

Pasquale

Diamanti

et al. 2017

Materials Science and Engineering: C

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The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37°C for 72h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of S. aureus (Gram-positive) and E. coli (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the S. aureus strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.

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“…8 The development of drug delivery systems, and in particular the surface delivery of bioactive agents, especially from nanoparticles, [16][17][18][19][20][21] is currently of interest for its obvious medical and hygiene applications [22][23][24] especially in the area of antibacterials 25 ; recent work is noteworthy in that regard. 26,27 Inspiration for the work reported herein came from a report in which small anions were found to bind reversibly with spiropyrans, 28,29 and of interest was whether a similar phenomenon might also bind carboxylates, and therefore penicillin, which would in turn impart bactericidal properties to a supporting polymer; the delivery of penicillin from polymers has been reported. 30 Even if such discrete one-to-one binding of carboxylate with spiropyrans did not occur, nonspeci¯c binding resulting from the change in surface polarity from the presence of a spiropyran might have been expected to lead to a similar outcome.…”

mentioning

confidence: 88%

Antibacterial Drug Releasing Materials by Post-Polymerization Surface Modification

Chng

Moloney

2017

J. Mol. Eng. Mater.

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Functional materials are available by the post-polymerization surface modi¯cation of diverse polymers in a three-step process mediated,¯rstly, by carbene insertion chemistry, secondly, by diazonium coupling, and thirdly by modi¯cation with a remotely tethered spiropyran unit, and these materials may be used for the reversible binding and release of Penicillin V. Surface loading densities of up to 0.19 mmol/g polymer are achievable, leading to materials with higher loading densities and release behavior relative to unmodi¯ed controls, and observable antibacterial biocidal activity.

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Controlling cell adhesion using layer-by-layer approaches for biomedical applications

Cited by 91 publications

References 185 publications

Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing: Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria

Antibacterial Drug Releasing Materials by Post-Polymerization Surface Modification

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