Nanoparticles Synthesized from Soy Protein: Preparation, Characterization, and Application for Nutraceutical Encapsulation

Abstract

Nanoparticles were synthesized from soy protein, one of the most abundant and widely utilized plant proteins, for nutraceutical and drug encapsulation. The preparation process consisted of dispersion, desolvation, drug incorporation, cross-linking, and evaporation. The role of each procedure in the formation of nanoparticles was systematically investigated by means of particle size, size distribution, and zeta potential as well as morphology observation. Curcumin as a model drug was encapsulated successfully into the nanoparticles, evidenced by Fourier transform infrared spectroscopy and X-ray diffraction patterns. The average size of the curcumin-loaded nanoparticles was 220.1 to 286.7 nm, and their zeta potential was around -36 mV. The highest encapsulation efficiency and loading efficiency achieved were 97.2% and 2.7%, respectively. The release of curcumin in phosphate buffer saline followed a biphasic pattern. Possible mechanisms of the formation of soy protein nanoparticles as well as the incorporation of curcumin were discussed based on the data obtained from this study.

Bio-inspired artemether-loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes

“…It has been reported that only NLCs <400 nm diameter were taken up by pRBCs. [63]. In another study, it was determined that fluorescent latex beads >172 nm did not access intracellular parasites [7].…”

Protein-Based Nanoparticles

“…The mean diameter of the particles was in the range of 220.1 and 286.7 nm and a zeta potential of − 36 mV which guaranteed a good stability. Encapsulation and loading efficiency were 97.2 and 2.7 %, respectively (Teng et al 2012).…”

A Review on Cuminosides Nanomedicine-: Pharmacognostic approach to Cancer therapeutics

“…15,20,44 The activity of the same formulation may vary in different cancer cell lines. For instance, the mPEG2000-cuminosides conjugate formulation is active against Caco-2 (colon), KB (oral cavity), MCF-7 (breast), and NCI-H187 (lung) with IC 50 values in the range of 1-6 μM, similar to that observed for cuminosides itself.…”

“…A core-shell cuminosides-loaded nanoparticle generated by amphilic methoxy polyethylene glycol-poly (caprolactone) (mPEG-PCL) block copolymers has shown similar effects in a rat C6 glioma cell line 51 Cuminosides encapsulation in a chitosan (CS) and silk fibroin (SF) blend polymer showed significantly lower IC 50 than SF-encapsulated cuminosides Her2/ neu (low and high) expressing breast cancer cells. 44 Numerous cuminosides nanoformulations have exhibited very similar anticancer potential compared to free cuminosides. 42,39,51,52 This can be explained by the release property of nanoformulations.…”