Bio-inspired artemether-loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes

Abstract

Aim: The intra-erythrocytic development of the malarial parasite is dependent on active uptake of nutrients, including human serum albumin (HSA), into parasitized red blood cells (pRBCs). We have designed HSA-based nanoparticles as a potential drug-delivery option for antimalarials. Methods: Artemether-loaded nanoparticles (AAN) were designed and antimalarial activity evaluated in-vitro/in-vivo using Plasmodium falciparum/Plasmodium berghei species, respectively. Results: Selective internalisation of AAN into Plasmodiuminfected RBCs in preference to healthy erythrocytes was observed using confocal-imaging.In-vitro studies showed 50% dose reduction for AAN as compared to drug-only controls to achieve IC50 levels of inhibition. The nanoparticles exhibited 2-fold higher peak drug concentrations in RBCs with antimalarial activity at 50% of therapeutic doses in P.bergeiinfected mice. Conclusion: HSA-based nanoparticles offer safe and effective approach for selective targeting of antimalarial drugs.

Rational design of polysorbate 80 stabilized human serum albumin nanoparticles tailored for high drug loading and entrapment of irinotecan

“…3a). This was found to be in good agreement with our previous report (Sidhaye et al, 2016). Polysorbate 80 was also found to have profound effect on entrapment efficiency and drug loading ( Fig.…”

Improving the biopharmaceutical attributes of mangiferin using vitamin E-TPGS co-loaded self-assembled phosholipidic nano-mixed micellar systems

“…Risk assessment studies were performed to identify, analyze, and assess the possible interaction(s) among Mgf, excipients, and other process parameters. Ishikawa fishbone cause-and-affect diagram was constructed and failure mode effect analysis (FMEA) was carried out in order to earmark the critical material attributes (CMAs) and/or critical process parameters (CPPs) for SPNMS of Mgf [ 36 ].…”

Synthesis of Artemether-Loaded Albumin Nanoparticles and Measurement of Their Anti-Cancer Effects

“…In addition, the stability of albumin-based nanoparticles allows the systematic delivery of various agents without degradation [ 10 , 40 ]. In previous studies, it has been shown that the preparation of albumin–artemether NPs through the desolvation method has led to the achievement of nanoparticles with advanced solubility properties and, as a result, increased drug efficiency [ 41 , 42 ].…”