Nanoparticles of pH-Responsive, PEG–Doxorubicin Conjugates: Interaction with an in Vitro Model of Lung Adenocarcinoma and Their Direct Formulation in Propellant-Based Portable Inhalers

Abstract

Pulmonary administration of polymer drug conjugates is of great potential clinical significance for treating lung cancer as such regimen significantly increases local drug concentrations while decreases systemic and local side effects. In this work, we demonstrate that nanoparticles prepared with methoxypoly(ethylene glycol) (mPEG)-doxorubicin (DOX) conjugates (mPEG-DOX) that have a pH-sensitive imine bond (Schiff base) can at the same time work as efficient carriers for DOX to kill cancer cells and also as a strategy to directly formulate nanoparticles in propellant-based inhalers. Nanoparticles prepared by precipitation in water had a diameter in the range between 100 and 120 nm. We investigated the effects of molecular weight (MW) of mPEG (1K, 2K, and 5K Da) on the in vitro release kinetics, cellular internalization, and cytotoxicity on in vitro model of lung adenocarcinoma and aerosol characters. It is observed that the DOX released from mPEG-DOX nanoparticles was significantly accelerated in acidic environment, pH 5.5 (endosomal/lysosomal pH) in comparison with pH 7.4 (physiological pH), as designed. Release of DOX from mPEG1K-DOX nanoparticles was significantly greater than those from mPEG2K and mPEG5K counterparts. In vitro cytotoxicity of nanoparticles followed the sequence of mPEG1K-DOX > free DOX > mPEG2K-DOX ≫ mPEG5K-DOX, a trend closely following their rate and extent of cellular internalization. mPEG-DOX nanoparticles with mPEG1K and mPEG2K were directly dispersed in hydrofluoroalkane (HFA), while a trace of ethanol was required to disperse mPEG5K-DOX nanoparticles in HFA. These pMDI formulations with high physical stability in HFAs display superior aerosol characteristics conducive to deep lung deposition. The fine particle fractions of these formulations ranged from 40-60%, higher than those of commercial products. Such formulations prepared from nanoparticles of pH-sensitive PEG-drug conjugates may also be envisioned to be extended to formulate other hydrophobic drugs for local delivery with propellant-based inhalers to other pulmonary disorders, thus broadening the impact of the proposed strategy.

Nanotechnology-Assisted Metered-Dose Inhalers (MDIs) for High-Performance Pulmonary Drug Delivery Applications

“…Rao et al (2017) prepared pH-sensitive NPs using methoxy-PEG (mPEG)-doxorubicin (DOX) conjugates (mPEG-1 K DOX) for effective treatment of lung cancer and directly formulate in propellant-MDIs to achieve site-specific action. The pH-sensitive mPEG-1 K DOX conjugated NPs were fabricated using the precipitation method and Schiff base re-arrangement technique ( 77 , 78 ). The NPs showed mean particle size and DOX loading of 104 nm and 32.7%, respectively.…”

Synthesis of Novel Tamarind Gum-co-poly(acrylamidoglycolic acid)-Based pH Responsive Semi-IPN Hydrogels and Their Ag Nanocomposites for Controlled Release of Chemotherapeutics and Inactivation of Multi-Drug-Resistant Bacteria

“…Doxorubicin (DOX) is a commonly used chemotherapeutic drug used to treat a variety of cancers, including hematological malignancies and soft tissue sarcomas [ 19 , 20 , 21 , 22 ]. It is obtained from a streptomyces species and belongs to the anthracycline family, which has potential antibacterial and anticancer action [ 23 , 24 ].…”

Synthesis of Novel Tamarind Gum-co-Poly(Acrylamidoglycolic Acid) based pH Responsive semi-IPN Hydrogels and their Ag Nanocomposites for Controlled Release of Chemotherapeutics and Inactivation of Multi Drug Resistance Bacteria

“…Simvastatin based nanoparticles formulated from polyelectrolyte complexes of chitosan and TMG for anti-cancer applications [19]. Doxorubicin (DOX) is a commonly used chemotherapeutic drug used to treat a variety of cancers, including haematological malignancies and soft tissue sarcomas [20][21][22][23]. It is obtained from a streptomyces species and belongs to the anthracycline family, which has potential antibacterial and anticancer action [24,25].…”