Nanoparticles based on β‐conglycinin and chitosan: Self‐assembly, characterization, and drug delivery

Abstract

The purpose of this study was to fabricate and evaluate nanoparticles based on β‐conglycinin (7S) and chitosan (CS) to deliver 5‐fluorouracil (5‐FU). The nanoparticles were prepared with a self‐assembly method. Turbidity measurements performed at 600 nm were used to investigate the formation of the nanoparticles as a function of the pH, 7S‐to‐CS mass ratio, and total concentration of 7S and CS. The optimum conditions for the preparation of the nanoparticles were a pH of 5.5, a 7S‐to‐CS mass ratio of 4 : 1, and total concentration of 7S and CS of 9 mg/mL. Under these conditions, the nanoparticles in solution had a high turbidity and good stability. Fourier transform infrared spectroscopy revealed that the nanoparticles were formed mainly through electrostatic interactions between the amine groups (NH3+) of CS and the carboxyl groups (COO−) of 7S. Scanning electron microscopy micrographs and dynamic light scattering analysis showed that the nanoparticles had an approximately spherical morphology with a smooth surface, and the mean particle size was about 120 nm with a narrow size distribution. The release of 5‐FU showed an initial burst release followed by a sustained release, and the release was pH‐dependent. The release mechanism of 5‐FU was Fickian diffusion according to the Ritger–Peppas model. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41963.

Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices

“…3,5 Moreover, chitosan is a renewable, biodegradable, biocompatible and biofunctional material that has been extensively used for different applications such as pesticide control, 6 removal of heavy metals 7 and drug delivery. 8,9 The versatility of chitosan as an immobilization support has been successfully demonstrated by different authors for sensing studies at a microfluidic scale. [10][11][12] Microfluidic paper-based analytical devices (µPADs) represent a new class of disposable devices that has been extensively investigated since the pioneering development by the Whitesides group.…”

Nanoparticles based on sodium alginate and β-conglycinin: Self-assembly and delivery of Phyllanthus urinaria phenolic compounds

“…In the spectrum B, the bands at 3,413, 1,647, 1,531, and 1,239 cm −1 were assigned to the stretching vibrations of O–H, C=O stretching (amide I), N–H (amide II), and C–H (amide III), respectively, (Teng, Luo, & Wang, ). Compared with the spectra of A and B, the spectrum of C showed some differences: the stretching vibrations of O–H and C=O (amide I) shifted to low wavenumber, from 3,478 and 3,413 cm −1 to 3,294 cm −1 and from 1,649, and 1647 cm −1 to 1643 cm −1 , explained by the presence of intermolecular hydrogen bonds in the nanoparticles (Liu, Wei et al, ); the intensity of the peaks of 1,446 and 1,240 cm −1 arose, and a new peak (1,339) appeared clearly, confirmed by the presence of phenyl–OH structures, which meant that the phenolic compounds were successfully loaded in the nanoparticles (Pasukamonset et al, ; Pawlaczyk‐Graja, ).…”

“…It is used as a folk medicine to treat various diseases, such as diarrhea, acute conjunctivitis, hepatitis, and edema (Huang et al, ) for its biological and pharmacological activities including antioxidant (Lin, Wang, Lu, Wu, & Hou, ), anti‐inflammatory (Kassuya, Leite, de‐Melo, Rehder, & Calixto, ), and antiviral (Hau, Gambari, & Wong, ) effects. In recent years, much attention has been paid to phenolic compounds from P. urinaria because they have the anticancer effect (Huang, Yang, Chen, & Pang, ; Huang et al, ) and can effectively treat liver cancer and hepatitis B virus (Lin, Yuan, Zhang, & Shi, ; Liu, Wei et al, ; Wang, Lin, Wu, Li, & Zhang, ; Xu et al, ; Yan, Sun, Yang, Bi, & Chen, ). However, the phenolic compounds, as the natural extracts, are susceptible to environmental factors such as light, oxidant, metal ions, and heat, which can reduce their stability and biological efficiency.…”