Nanomaterials integrated with microfluidic paper-based analytical devices for enzyme-free glucose quantification

Khachornsakkul, Kawin; Rybicki, Frank J.; Sonkusale, Sameer

doi:10.1016/j.talanta.2023.124538

Cited by 14 publications

(11 citation statements)

References 47 publications

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“…The synthesis of AuNPs was performed according to a previous procedure with slight modifications . Here, 50.0 mL of HAuCl 4 at 40.0 mmol/L was heated under constant stirring for 3 h. Subsequently, 2.0 mL of 1.0% (w/v) sodium citrate was slowly introduced in the presolution under heating and stirring for 30.0 min.…”

Section: Methodsmentioning

confidence: 99%

“…The synthesis of AuNPs was performed according to a previous procedure with slight modifications. 34 Here, 50.0 mL of HAuCl 4 at 40.0 mmol/L was heated under constant stirring for 3 h. Subsequently, 2.0 mL of 1.0% (w/v) sodium citrate was slowly introduced in the presolution under heating and stirring for 30.0 min. Once the solution turns red, indicating the presence of AuNPs in the solution, 0.20 mL of PVA is injected into the solution to enhance the stability of the AuNPs.…”

Section: Synthesis Of Aunps and Conjugation Of Antibodiesmentioning

confidence: 99%

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Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Khachornsakkul,

Del-Rio-Ruiz,

Zeng

et al. 2023

Anal. Chem.

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Herein, we successfully developed a thread-based analytical device (μTAD) for simultaneous immunosensing of two biomolecules with attomolar sensitivity by using a photothermal effect. A photothermal effect exploits a strong light-to-heat energy conversion of plasmonic metallic nanoparticles at localized surface plasmon resonance. The key innovation is to utilize the cotton thread to realize this sensor and the use of chitosan modification for enhancing the microfluidic properties, for improving the efficiency of photothermal conversion, and for sensor stability. The developed μTAD sensor consists of (i) a sample zone, (ii) a conjugation zone coated with gold nanoparticles bound with an antibody (AuNPs-Ab 2 ), and (iii) a test zone immobilized with a capture antibody (anti-Ab 1 ). The prepared μTAD is assembled in a custom three-dimensional (3D) printed device which holds the laser for illumination and the thermometer for readout. The 3D-printed supportive device enhances signal response by focusing light and localizing the heat generated. For proof of concept, simultaneous sensing of two key stress and inflammation biomarkers, namely, cortisol and interleukin-6 (IL-6), are monitored using this technique. Under optimization, this device exhibited a detection linear range of 2.0−14.0 ag/mL (R 2 = 0.9988) and 30.0−360.0 fg/mL (R 2 = 0.9942) with a detection limit (LOD) of 1.40 ag/mL (∼3.86 amol/L) and 20.0 fg/mL (∼950.0 amol/L) for cortisol and IL-6, respectively. Furthermore, the analysis of both biomolecules in human samples indicated recoveries in the range of 98.8%−102.88% with the highest relative standard deviation being 3.49%, offering great accuracy and precision. These results are the highest reported sensitivity for these analytes using an immunoassay method. Our PT-μTAD strategy is therefore a promising approach for detecting biomolecules in resource-limited point-of-care settings.

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Section: Methodsmentioning

confidence: 99%

Section: Synthesis Of Aunps and Conjugation Of Antibodiesmentioning

confidence: 99%

Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Khachornsakkul,

Del-Rio-Ruiz,

Zeng

et al. 2023

Anal. Chem.

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“…15 μPADs have effectively integrated with diverse detection technologies. Specifically, when coupled with colorimetric techniques, 16,17 they have been tailored for the detection of analytes like glucose, lactic acid, and uric acid. 18,19 This strategy achieves quantitative analysis by monitoring the color changes induced by the target substances on the μPAD.…”

Section: ■ Introductionmentioning

confidence: 99%

“…μPADs have effectively integrated with diverse detection technologies. Specifically, when coupled with colorimetric techniques, , they have been tailored for the detection of analytes like glucose, lactic acid, and uric acid. , This strategy achieves quantitative analysis by monitoring the color changes induced by the target substances on the μPAD. The amalgamation of μPADs with electrochemical technology is applied to the detection of heavy metals, dopamine, cortisol, and various other analytes. − By employing the printing of a three-electrode system on the paper and modifying sensing materials, quantitative analysis is achieved through the measurement of current changes resulting from the interaction between the analyte and the electrodes .…”

Section: Introductionmentioning

confidence: 99%

Paper-Based Microfluidic Sensors Utilizing Metal–Organic Framework Materials Modified with Europium and Carbon Quantum Dots for Anthrax Spore Biomarker Detection

Wang,

Mei,

et al. 2024

ACS Appl. Nano Mater.

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The germination of bacterial spores presents a considerable challenge to health and safety due to the current scarcity of cost-effective and efficient tools for their prompt identification. Addressing this critical need, our study introduces a novel paper-based ratiometric fluorescence sensor platform utilizing a composite of Eu 3+ -doped carbon quantum dots (CQDs) within ZIF-8 (Eu 3+ -CQDs@ZIF-8). This platform facilitates the rapid detection of 2, 6-pyridinedicarboxylic acid (DPA), a biomarker of bacterial spore germination. To fabricate this sensor, CQDs and Eu 3+ ions were synergistically integrated into the ZIF-8 framework. The resultant composite was then applied to filter paper via screen printing technology, creating a paper-based microfluidic analytical device (μPAD). During the process of detecting DPA, strong coordination between DPA and Eu 3+ ions is established, resulting in an enhanced red emission due to energy transfer through the antenna effect. Concurrently, the interaction between DPA and CQDs leads to a quenching of the CQDs' green fluorescence, enabling a ratiometric detection of DPA. The efficacy of this composite material was validated through the visualization of DPA release during spore germination. Further enhancing the platform's utility, integration with a smartphone enabled the quantification of DPA concentrations in real samples by analyzing the red-to-green (R/G) value in the detection area. Demonstrating rapid, sensitive, and precise DPA detection, the platform confirms its suitability for practical applications. Conclusively, with its compact design, straightforward preparation, and comprehensive integration, this sensor platform emerges as a promising solution for on-site spore detection, highlighting its potential for widespread testing applications.

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“…Sensitive materials can react with target molecules and produce physical and chemical changes, including optical, acoustic, electrical, magnetic, thermal, and mechanical signals. Based on different signals, various detection technologies have been developed in recent years, including spectroscopic methods, such as fluorescence, surface plasmon resonance [ 12 ], colorimetric analysis [ 13 , 14 , 15 , 16 , 17 ], and illuminating sensors [ 18 , 19 , 20 , 21 ]. However, these spectroscopic methods usually need complex instruments and professional personnels, which are not suitable for household use but scientific research institutions and hospitals.…”

Section: Introductionmentioning

confidence: 99%

Research Progress on Biomimetic Nanomaterials for Electrochemical Glucose Sensors

Chi

Zhang

et al. 2023

Biomimetics

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Diabetes has become a chronic disease that necessitates timely and accurate detection. Among various detection methods, electrochemical glucose sensors have attracted much attention because of low cost, real-time detection, and simple and easy operation. Nonenzymatic biomimetic nanomaterials are the vital part in electrochemical glucose sensors. This review article summarizes the methods to enhance the glucose sensing performance of noble metal, transition metal oxides, and carbon-based materials and introduces biomimetic nanomaterials used in noninvasive glucose detection in sweat, tear, urine, and saliva. Based on these, this review provides the foundation for noninvasive determination of trace glucose for diabetic patients in the future.

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Nanomaterials integrated with microfluidic paper-based analytical devices for enzyme-free glucose quantification

Cited by 14 publications

References 47 publications

Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Highly Sensitive Photothermal Microfluidic Thread-Based Duplex Immunosensor for Point-of-Care Monitoring

Paper-Based Microfluidic Sensors Utilizing Metal–Organic Framework Materials Modified with Europium and Carbon Quantum Dots for Anthrax Spore Biomarker Detection

Research Progress on Biomimetic Nanomaterials for Electrochemical Glucose Sensors

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