Nanoinjector for Capillary Electrophoresis and Capillary Electrochromatography

Iizuka, Eiji; Tsuda, Toshitaka; Munesue, Motonori; Samizo, Shinichi

doi:10.1021/ac020778y

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Either classically-driven injection technique of pressure or voltage [hydrodynamic (HD) and electrokinetic (EK)] are found used in CE instruments (bench-top and P-CE) [68]. For opti-mal mechanical rigidity, the P-CE design needs to be protected from unexpected manipulation.…”

Section: Injection Systemsmentioning

confidence: 99%

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Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

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Section: Injection Systemsmentioning

confidence: 99%

“…The main advantage of this injector was the possibility of injection under a continuously applied voltage. Since it is technically difficult to make a very narrow sample chamber using conventional machining, Iizuka et al [68] embedded a fused-silica capillary tubing with i.d. 50-100 m in a resin-housed rotor.…”

Section: Injection Valvesmentioning

confidence: 99%

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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“…Full loop injections could be performed reproducibly, while high voltage was applied. As the injected volume (350 nL) was still rather large for CE, a nanoinjector was developed with an internal loop of only 6 nL [143]. Similarly, Ponton and Evans [144] used a commercially available valve for sample introduction, originally developed for capillary-LC purposes.…”

Section: Valve Interfacesmentioning

confidence: 99%

Design and applications of coupled SPE‐CE

et al. 2007

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CE suffers from an inherent low concentration sensitivity. Analyte detection limits can be improved by combining CE with SPE. This paper presents an overview of coupled SPE-CE systems that have been reported in literature. Attention is paid to fundamental aspects of coupling SPE and CE, as well as to important SPE requirements. Interfaces for inline and online coupling with CE are critically discussed, and their mode of operation is outlined. Advantages and limitations of the interfaces are discussed and typical examples are selected. Finally, some future developments are discussed.

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“…In 2003, Iizuka et al . () improved the rotary‐type injector using a piece of fused‐silica capillary that served as a sample chamber of 6–24 nL. In spite of the highly accurate and reliable quantitation, it was hard for the system to reach large‐scale production because it was difficult to manufacture the precision valve.…”

Section: Introductionmentioning

confidence: 99%

Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability

Ling

Zhu

et al. 2015

Biomedical Chromatography

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A quantitative capillary electrophoresis (qCE) was developed by utilizing a rotary type of nano-volume injector, an autosampler, and a thermostat with cooling capacity. The accuracy and precision were greatly improved compared with conventional capillary electrophoresis. The 10 nL volume accuracy was guaranteed by the carefully designed nano-injector with an accurate internal loop. The system repeatability (precision) in terms of RSD <0.5% for migration time and 1% for peak area were achieved by using DMSO as a test sample. We believe that this fully automated qCE system has the potential to be employed broadly in quality control and quality assurance in the pharmaceutical industry.

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Nanoinjector for Capillary Electrophoresis and Capillary Electrochromatography

Cited by 14 publications

References 18 publications

Portable capillary-based (non-chip) capillary electrophoresis

Portable capillary-based (non-chip) capillary electrophoresis

Design and applications of coupled SPE‐CE

Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability

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