Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability

Xu, Yifeng; Ling, Bangzan; Zhu, Wenjun; Yao, Dongmei; Zhang, Lin; Wang, Yan; Yan, Chao

doi:10.1002/bmc.3560

Cited by 4 publications

(3 citation statements)

References 21 publications

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“… and Xu et al. developed a quantitative capillary electrophoresis (qCE) system (Fig. ) in order to improve the accuracy and precision of the currently commercially available CE.…”

Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

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“… and Xu et al. developed a quantitative capillary electrophoresis (qCE) system (Fig. ) in order to improve the accuracy and precision of the currently commercially available CE.…”

Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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“…However, CE has often been considered to have inferior reproducibility compared to other separation techniques such as HPLC or gas chromatography (GC) due to sample injection bias, sample leakage and other factors inherently induced by current sample injection methods, and thus has not been as widely used in quantitative analysis [25–27]. Numerous advances have largely eliminated this concern in recent years [17,28], though achieving the desired degree of reproducibility (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Such method has not been directly applicable to CE due to the much smaller sample volume requirements of CE as well as issues arising from the use of high voltages. However, by developing an electrical decoupler, a similar approach has been shown in conventional, macroscale, CE systems [25,30]. Sample was loaded into a nanoliter-scale injection valve, then pushed via a syringe pump through the electrical decoupler into the separation channel, after which the CE voltage can be applied.…”

Section: Introductionmentioning

confidence: 99%

Novel volumetric method for highly repeatable injection in microchip electrophoresis

Jones

et al. 2017

Analytica Chimica Acta

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A novel injector for microchip electrophoresis (MCE) has been designed and evaluated that achieves very high repeatability of injection volume suitable for quantitative analysis. It eliminates the injection biases in electrokinetic injection and the dependence on pressure and sample properties in hydrodynamic injection. The microfluidic injector, made of poly(dimethylsiloxane) (PDMS), operates similarly to an HPLC injection valve. It contains a channel segment (chamber) with a well-defined volume that serves as an “injection loop”. Using on-chip microvalves, the chamber can be connected to the sample source during the “loading” step, and to the CE separation channel during the “injection” step. Once the valves are opened in the second state, electrophoretic potential is applied to separate the sample. For evaluation and demonstration purposes, the microinjector was connected to a 75 µm ID capillary and UV absorbance detector. For single compounds, a relative standard deviation (RSD) of peak area as low as 1.04% (n=11) was obtained, and for compound mixtures, RSD as low as 0.40% (n=4) was observed. Using the same microchip, the performance of this new injection technique was compared to hydrodynamic injection and found to have improved repeatability and less dependence on sample viscosity. Furthermore, a non-radioactive version of the positron-emission tomography (PET) imaging probe, FLT, was successfully separated from its known 3 structurally-similar byproducts with baseline resolution, demonstrating the potential for rapid, quantitative analysis of impurities to ensure the safety of batches of short-lived radiotracers. Both the separation efficiency and injection repeatability were found to be substantially higher when using the novel volumetric injection approach compared to electrokinetic injection (performed in the same chip). This novel microinjector provides a straightforward way to improve the performance of hydrodynamic injection and enables extremely repeatable sample volume injection in MCE. It could be used in any MCE application where volume repeatability is needed, including the quantitation of impurities in pharmaceutical or radiopharmaceutical samples.

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An open source 3D printed autosampler for capillary electrophoresis

Itterheimová,

Kubáň

2023

Analytica Chimica Acta

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Development of fully automated quantitative capillary electrophoresis with high accuracy and repeatability

Cited by 4 publications

References 21 publications

Recent advances in microscale separation

Recent advances in microscale separation

Novel volumetric method for highly repeatable injection in microchip electrophoresis

An open source 3D printed autosampler for capillary electrophoresis

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