Nanoimmunoassay onto a screen printed electrode for HER2 breast cancer biomarker determination

A novel antibody–antigen based impedimetric immunosensor for low level detection of HER2 in serum samples of breast cancer patients via modification of a gold nanoparticles decorated multiwall carbon nanotube-ionic liquid electrode

“…It should be noted the limit of detection of the proposed method (i.e., 7.2 ng mL -1 ) is more or less close to the value of 6 ng mL -1 reported for an immunosensor working based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein Amodified magnetic beads [21], while it is much improved relative to a LOD of 1 µg mL -1 reported for a screen printed electrode based HER2 immunosensor [25]. Meanwhile, the LODs already reported for an impedance aptasensor developed by immobilizing HER2-specific single stranded DNA aptamer onto the monolayer of 3-mercaptopropionic acid self-assembled on gold nanoparticles electrode [23] and an electrochemical immunosensor based on antiHER2-iron oxide nanoparticle bioconjugates for detection of breast cancer biomarker [24] are significantly improved, as compared to this work.…”

“…Among these, the electrochemical immunosensors have been well established as valid alternatives to classical analysis methods for detection of cancer markers, by offering the advantages of being easy to use, rapid, robust and often inexpensive and capable of multi-analyte testing [19]. Thus, in recent years, several electrochemical [20][21][22][23][24][25] and optical [26,27] immunosensors have been proposed for the detection of HER2.…”

Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors

“…The HER2 concentration in the serum of breast cancer patients is 15-75 ng/mL, which is elevated when compared to that of normal individuals (2-15 ng/mL) [8]. Monitoring the level of HER2 protein could also be a good indicator of antitumor treatment efficiency [9]. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are the most commonly used methods for HER2 analysis.…”

A label-free electrochemical affisensor for cancer marker detection: The case of HER2

“…The DNA aptamer was immobilized on the nanostructured gold electrode surface; HER2 biomarker in buffered solutions was then determined by EIS measurements in a linear range between 10 −5 and 100 ng/mL. Patris et al [32] reported the use of a new class of bioreceptors called Nanobodies® (Nbs). The proposed immunoassay was based on the immobilization of a primary anti-HER2 nanobody on the surface of graphite screen-printed electrode followed by the reaction with the antigen and with a horseradish peroxidase (HRP)-labeled secondary anti-HER2 nanobody.…”