A label-free electrochemical affisensor for cancer marker detection: The case of HER2

Ravalli, Andrea; Rocha, Carolina Gomes da; Yamanaka, Hideko; Marrazza, Giovanna

doi:10.1016/j.bioelechem.2015.07.010

Cited by 91 publications

(60 citation statements)

References 39 publications

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“…As examples of sensors using AuNPs, Ravalli et al. developed a sensitive biosensor based on easy immobilization of the bioreceptor on AuNPs, electrodeposited on screen‐printed graphite. Marques et al.…”

Section: Electrode Surface Modificationmentioning

confidence: 99%

“…In this strategy, distinct carbon‐ and gold‐ based nanomaterials are typically used as platforms for the efficient binding of antibodies. The interaction between antibodies and gold nanomaterials (mainly AuNPs) are usually achieved by chemisorption via thiol derivatives, involving a chemical adsorption process between the nanomaterial surface and the adsorbent surface, which causes the bond to be created , . As an example, Wang et al.…”

Section: Antibody Immobilizationmentioning

confidence: 99%

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Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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Section: Electrode Surface Modificationmentioning

confidence: 99%

Section: Antibody Immobilizationmentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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“…A tumor marker, human epidermal growth factor receptor 2 (HER2), was the target of a sensitive label-free impedimetric biosensor developed by Ravalli et al [68] The authors used in this case an affibody, a protein generated using a combinatorial protein engineering approach for the target analyte. These molecules represent new binders able to stick to different target proteins, retaining the favorable folding and stability properties.…”

Section: Biosensorsmentioning

confidence: 99%

Chemical Sensors and Biosensors in Italy: A Review of the 2015 Literature

Compagnone

Francia

Natale

et al. 2017

Sensors

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The contributions of Italian researchers to sensor research in 2015 is reviewed. The analysis of the activities in one year allows one to obtain a snapshot of the Italian scenario capturing the main directions of the research activities. Furthermore, the distance of more than one year makes meaningful the bibliometric analysis of the reviewed papers. The review shows a research community distributed among different scientific disciplines, from chemistry, physics, engineering, and material science, with a strong interest in collaborative works.

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“…For these reasons, the development of an easy-to-use, fast and accurate device for allergen detection is fundamental to prevent life-threatening reactions in allergic people. In this context, biosensors are a valid alternative to conventional detection methods and they have attracted considerable interest in the last decades [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer

et al. 2019

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In this work, we present a new electrochemical disposable platform based on poly(aniline‐co‐anthranilic acid) (PANI/PAA) composite polymer coupled with an aptamer for sensitive detection of β‐lactoglobulin. Firstly, PANI/PAA film was electrodeposited on the graphite screen‐printed electrode surface by cyclic voltammetry. The co‐polymer modified electrode was then employed as platform for the covalent immobilization of an amino‐modified aptamer. Various β‐lactoglobulin solutions, with a fixed amount of biotinylated oligonucleotide complementary sequence, were dropped onto the aptasensor surface. A streptavidin‐alkaline phosphatase conjugate was then employed to trace the affinity reaction. After the addition of 1‐naphthyl‐phosphate enzymatic substrate, 1‐naphthol electroactive product was detected by differential pulse voltammetry. A decrease in the signal was obtained when the target concentration was increased, in according to a signal‐off approach. After optimization of key experimental parameters, a dose‐response curve was obtained between 0.01–1.0 μg mL−1 β‐lactoglobulin concentration range. The limit of detection of 0.053 μg L−1 was obtained. Milk samples spiked with β‐lactoglobulin were analyzed.

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A label-free electrochemical affisensor for cancer marker detection: The case of HER2

Cited by 91 publications

References 39 publications

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

Chemical Sensors and Biosensors in Italy: A Review of the 2015 Literature

Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer

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