A novel antibody–antigen based impedimetric immunosensor for low level detection of HER2 in serum samples of breast cancer patients via modification of a gold nanoparticles decorated multiwall carbon nanotube-ionic liquid electrode

Arkan, Elham; Saber, Reza; Karimi, Ziba; Shamsipur, Mojtaba

doi:10.1016/j.aca.2015.03.022

Cited by 147 publications

(61 citation statements)

References 38 publications

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“…The developed configuration involved the use of additional AuNPs immobilized onto AuNPs/MW-CILE through 1,6-hexanedithiol to improve the extent of immobilization and stability of Herceptin antibody. Monitoring of immunocomplex formation with different amounts of HER2 was made via the changes of impedance responses, these providing linear increases in a clinically useful concentration range [43]. Another representative immunosensor was constructed for carcinoma antigen-125 (CA-125), a member of the mucin family of glycoproteins that constitutes a standard clinical biomarker associated with gynecological malignancies [44].…”

Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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Early diagnosis is often the key to successful patient treatment and survival. The identification of various disease signaling biomarkers which reliably reflect normal and disease states in humans in biological fluids explain the burgeoning research field in developing new methodologies able to determine the target biomarkers in complex biological samples with the required sensitivity and selectivity and in a simple and rapid way. The unique advantages offered by electrochemical sensors together with the availability of high affinity and specific bioreceptors and their great capabilities in terms of sensitivity and stability imparted by nanostructuring the electrode surface with different carbon nanomaterials have led to the development of new electrochemical biosensing strategies that have flourished as interesting alternatives to conventional methodologies for clinical diagnostics. This paper briefly reviews the advantages of using carbon nanostructures and their hybrid nanocomposites as electrode modifiers to construct efficient electrochemical sensing platforms for diagnosis. The review provides an updated overview of some selected examples involving attractive amplification and biosensing approaches which have been applied to the determination of relevant genetic and protein diagnostics biomarkers.

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Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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“…Much attention has been focused on signal amplification using nanomaterials as labels to improve the sensitivity in immunoassays Wang et al, 2011Wang et al, , 2012. Gold nanoparticles (AuNPs) have been used in different immunoassays due to its unique physical and chemical properties, such as easily controllable size distribution, long-term stability, superior conductivity, and biocompatibility (Arkan et al, 2015;Liu et al, 2014;Mani et al, 2009). Moreover, hybrid materials also have caused extensive interest by combining the desirable properties of various materials for synergistic effects.…”

Section: Introductionmentioning

confidence: 99%

Branched zinc oxide nanorods arrays modified paper electrode for electrochemical immunosensing by combining biocatalytic precipitation reaction and competitive immunoassay mode

Sun

Yang

Zhang

et al. 2015

Biosensors and Bioelectronics

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“…Furthermore, the use of small-size transducers, the reasonably short assay times and the low reagent/sample volumes are key features for the development of point-of-care devices. Pre-synthesized AuNPs were grown on the MWCNT-CILE surface through electrodeposition, to form AuNP/MWCNT-CILE, which is a suitable surface for antibody attachment [13]. In a different approach Ravalli et al developed a label-free impedimetric biosensor based on the immobilization of a terminal cysteine-modified affibody on gold nanostructured graphite screen-printed electrodes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensing Platforms for HER2‐ECD Breast Cancer Biomarker Detection

2018

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Screening and early diagnosis are crucial to increase the success of cancer patients’ treatments and improve the survival rate. To contribute to this success, distinct electrochemical immunosensing platforms were developed for the analysis of the ExtraCellular Domain of the Human Epidermal growth factor Receptor 2 (HER2‐ECD) through sandwich assays on nanomaterial‐modified screen‐printed carbon electrodes (SPCEs). The most promising platforms showed to be SPCEs modified with (i) gold nanoparticles (AuNPs) and (ii) multiwalled carbon nanotubes combined with AuNPs. The antibody‐antigen interaction was detected using a secondary antibody labelled with alkaline phosphatase and 3‐indoxyl phosphate and silver ions as the enzymatic substrate. The electrochemical signal of the enzymatically generated metallic silver was recorded by linear sweep voltammetry. Under the optimized conditions, linear calibration plots were obtained between 7.5 and 50 ng/mL and the total assay time was 2 h 20 min, achieving LODs of 0.16 ng/mL (SPCE‐MWCNT/AuNP) and 8.5 ng/mL (SPCE‐AuNP), which are well below the established cut‐off value of 15 ng/mL for this cancer biomarker.

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A novel antibody–antigen based impedimetric immunosensor for low level detection of HER2 in serum samples of breast cancer patients via modification of a gold nanoparticles decorated multiwall carbon nanotube-ionic liquid electrode

Cited by 147 publications

References 38 publications

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Branched zinc oxide nanorods arrays modified paper electrode for electrochemical immunosensing by combining biocatalytic precipitation reaction and competitive immunoassay mode

Electrochemical Sensing Platforms for HER2‐ECD Breast Cancer Biomarker Detection

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