Nanohydroxyapatite–chitosan–gelatin polyelectrolyte complex with enhanced mechanical and bioactivity

Rajkumar, M.; Kandiah, Kavitha; Prabhu, M. Ramesh; Meenakshisundaram, N.; Rajendran, V.

doi:10.1016/j.msec.2013.04.005

Cited by 38 publications

(22 citation statements)

References 43 publications

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“…Among which, biomimetic nanohydroxyapatite/chitosan/gelatin (HaCG) scaffolds were a popular choice owing to their ability to stimulate constituent components of natural bone, guide the regeneration progress, and degrade to nontoxic byproducts 22 , 23 . Nanohydroxyapatite (HA), the core component of this composite biomaterial, has been shown not only to promote cell adhesion, and support long-term growth, but also to help induce increased cell proliferation and differentiation towards a bone lineage 24 , 25 . Chitosan possesses inherent physical and biological characteristics that render it useful as a components in bone tissue engineering 20 , 23 , 26 ; it even has a bactericidal effect 27 .…”

Section: Introductionmentioning

confidence: 99%

Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions

Wang

Liu

Xing

et al. 2017

Sci Rep

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Subchondral bone has been identified as an attractive target for KOA. To determine whether a minimally invasive micro-scaffolds could be used to induce regeneration of knee subchondral bone lesions, and to examine the protective effect of subchondral bone regeneration on upper cartilage, a ready-to-use injectable treatment with nanohydroxyapatite-chitosan-gelatin micro-scaffolds (HaCGMs) is proposed. Human-infrapatellar-fat-pad-derived adipose stem cells (IPFP-ASCs) were used as a cellular model to examine the osteo-inductivity and biocompatibility of HaCGMs, which were feasibly obtained with potency for multi-potential differentiations. Furthermore, a subchondral bone lesion model was developed to mimic the necrotic region removing performed by surgeons before sequestrectomy. HaCGMs were injected into the model to induce regeneration of subchondral bone. HaCGMs exhibited desirable swelling ratios, porosity, stiffness, and bioactivity and allowed cellular infiltration. Eight weeks after treatment, assessment via X-ray imaging, micro-CT imaging, and histological analysis revealed that rabbits treated with HaCGMs had better subchondral bone regeneration than those not treated. Interestingly, rabbits in the HaCGM treatment group also exhibited improved reservation of upper cartilage compared to those in other groups, as shown by safranin O-fast green staining. Present study provides an in-depth demonstration of injectable HaCGM-based regenerative therapy, which may provide an attractive alternative strategy for treating KOA.

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Section: Introductionmentioning

confidence: 99%

Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions

Wang

Liu

Xing

et al. 2017

Sci Rep

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“…Polyelectrolyte complex (PEC) nanoparticles have attracted more attentions due to their wide merits as drug carriers, and they are suitable for loading some charged drug molecules such as DNA and proteins with high loading efficiencies. The drug releases can be controlled by pH and ionic strength (Li et al, 2011;Zhao et al, 2013;Tang et al, 2003;Teng et al, 2013;Rajkumar et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The polyion complex nano-prodrug of doxorubicin (DOX) with poly(lactic acid-co-malic acid)-block-polyethylene glycol: preparation and drug controlled release

Zhang

Shi

et al. 2014

Med Chem Res

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A polyion complex nano-prodrug based on the complexation of the ionomer of poly(lactic acid-co-malic acid)-block-polyethylene glycol [Poly(LA-co-MA)-b-PEG] with doxorubicin (DOX) was developed. The ionomer was prepared by a direct polycondensation of D,L-lactic acid (LA), L-malic acid (MA), and monomethyl polyethylene glycol (PEG) using stannous chloride (SnCl 2 ) as the catalyst. The nanoparticles were formed through electrostatic interactions between the side carboxyl groups of MA units in the ionomer and amino groups of DOX. The nanoparticle possessed an amphiphilic structure with a hydrophobic core of the complexed DOX and Poly(LA-co-MA) and a hydrophilic shell of PEG. The nanoparticle solution was stable, and the particle sizes were in the range of 110-140 nm. The DOX was loaded as a prodrug with loading rate as high as 18.2 %. The drug release could be controlled, showing responsiveness of acids and ionic strength. The cumulative release was as high as 94 %. The nanoparticles could be potentially used as anti-cancer drug vehicles.

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“…[4][5][6][7] The relationship between lattice constant (a & c), Miller's indices (h,k,l) and lattice spacing (d) is used to calculate lattice parameter values, expressed as, Figure 1A & 1B). 8…”

Section: Xrd Analysismentioning

confidence: 99%

Synthesis and Structural Properties Characterization of HA/Alumina and HA/MgO Nanocomposite for Biomedical Applications

VIJAYALAKSHMI¹

2017

OAJTMR

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HA/Alumina and HA/MgO nanocomposites were successfully prepared by using the hydrothermal method for the first time at 250°C. The mechanisms of composite formation, crystallite size, crystallinity, morphology, were studied HA/Alumina and HA/MgO nanocomposites. XRD and FTIR investigations showed an intermolecular interaction between HA/Alumina and HA/MgO. The formation of HA/Alumina and HA/MgO nanocomposites are polycrystalline in nature. It confirmed in TEM analysis. TEM images further ascertained that HA P /Alumina was formed in a short nanorod shape and HA/MgO nanocomposites show nanocluster like morphology. The FTIR measurement shows the appropriate vibrational spectra of HA/Alumina and HA/MgO nanocomposites. The UV-VIS measurements show the band-gap of HA/Alumina and HA/MgO nanocomposites. This study provides a platform for further research on the HA/Alumina and HA/MgO nanocomposites for biomedical applications.

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Nanohydroxyapatite–chitosan–gelatin polyelectrolyte complex with enhanced mechanical and bioactivity

Cited by 38 publications

References 43 publications

Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions

Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions

The polyion complex nano-prodrug of doxorubicin (DOX) with poly(lactic acid-co-malic acid)-block-polyethylene glycol: preparation and drug controlled release

Synthesis and Structural Properties Characterization of HA/Alumina and HA/MgO Nanocomposite for Biomedical Applications

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