Nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/collagen/graphene oxide scaffolds for wound coverage

Zine, Rashtrapal; Sinha, Mukty

doi:10.1016/j.msec.2017.05.138

Cited by 48 publications

(34 citation statements)

References 24 publications

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“…FTIR spectroscopy was used to assess the physical and chemical interaction of the polymers. 25 Figure 3 shows the FTIR spectra of LID, chitosan, SLS, mupirocin, PCL, FLS, and DLS. As shown in Figure 3A, LID showed two characteristic bands around 3,385 and 1,686 cm −1 that could be attributed to N-H stretching and C=O stretching.…”

Section: Ftirmentioning

confidence: 99%

“…21,23 Compared with synthetic polymers, natural polymers exhibit low immunogenicity and better biocompatibility and can mimic the native extracellular matrix (ECM) of biological tissue. 14,21,24 Widely used natural polymers for wound healing include chitosan, 1,16 collagen, 25,26 alginate, 27 gelatin, 28 chitin, 29 and silk fibroin. 30 However, wound dressings prepared from natural polymers are unable to retain their structural stability, 31 while those from synthetic polymers usually have good mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Li¹,

Wang²,

Yang³

et al. 2018

IJN

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BackgroundAn ideal wound dressing should exhibit good biocompatibility, minimize pain and infection, absorb excess exudates, and maintain a moist environment. However, few clinical products meet all these needs. Therefore, the aim of this study was to fabricate a multifunctional double layer nanofibrous scaffolds (DLS) as a potential material for wound dressing.Materials and methodsThe scaffold was formed from mupirocin and lidocaine hydrochloride homogeneously incorporated into polycaprolactone as the first layer of scaffolds and chitosan as the second layer of scaffolds nanofibers through electrospinning. The fabricated nanofibrous scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, and measurement of swelling ratio, contact angle, drug release, and mechanical properties. Furthermore, antibacterial assessment, live/dead cell assays, and MTT assays were used to investigate the antibacterial activity and cytotoxicity of the nanofibrous scaffolds.ResultsThe morphology of the nanofibrous scaffolds was studied by scanning electron microscopy, showing successful nanofibrous scaffolds. Fourier transform infrared spectroscopy demonstrated the successful incorporation of the material used to produce the produced nanofibrous scaffolds. Thermal studies with thermogravimetric analysis and differential scanning calorimetry indicated that the DLS had high thermal stability. The DLS also showed good in vitro characteristics in terms of improved swelling ratio and contact angle. The mechanical results revealed that the DLS had an improved tensile strength of 3.88 MPa compared with the second layer of scaffold (2.81 MPa). The release of drugs from the scaffold showed different profiles for the two drugs. Lidocaine hydrochlo ride exhibited an initial burst release (66% release within an hour); however, mupirocin exhibited only a 5% release. Furthermore, the DLS nanofibers displayed highly effective antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa and were nontoxic to fibroblasts.ConclusionThe fabricated DLS exhibited excellent hydrophilicity, cytocompatibility, sustained drug release, and antibacterial activity, which are favorable qualities for its use as a multifunctional material for wound dressing applications.

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Section: Ftirmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Li¹,

Wang²,

Yang³

et al. 2018

IJN

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“…Collagen was included with the aim of enhancing cell proliferation, without affecting the composite materials mechanical strength or porosity. Subsequent biological results revealed that these nanofiber membranes generally had good wound healing properties [75] (Figure 11). , resulting in these materials displaying good antimicrobial properties against Pseudomonas aeruginosa and S. aureus [105] (Figure 12).…”

Section: Wound Dressing Applicationsmentioning

confidence: 99%

Electrospinning of Collagen and Its Derivatives for Biomedical Applications

Lü¹,

Guo²

2018

Novel Aspects of Nanofibers

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Collagen, gelatin and their derived polypeptides can act as multifunctional natural polymers with excellent physicochemical properties for biomedical applications. The use of electrospinning technology can convert collagen materials into nanofibrous materials that exhibit porous micro-nanostructures with good mechanical properties and excellent biocompatibility profiles. In this chapter, a systematic review of collagen electrospinning is presented and related applications are introduced including tissue engineering (e.g., artificial skin, artificial vasculature, cartilage repair, etc.), drug delivery, hemostatic dressings, periodontal restoration, biofilms, and wound dressings will now be discussed.

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“…Pure electrospun PHBV meshes supported the adhesion, growth, and epidermal differentiation of bone marrow mesenchymal stem cells, which was induced by an appropriate composition of cell culture media, containing epidermal growth factor, insulin, 3,3′,5-triiodo-L-thyronine (T3), hydrocortisone, and 1α, 25-dihydroxyvitamin (D3), and manifested by expression of genes for keratin, filaggrin, and involucrin, that is, an early, intermediate and late marker of keratinocyte differentiation, respectively [166]. In order to increase the attractiveness of electrospun PHBV nanofibers for the cell adhesion and growth, they were coated with collagen [167] blended with collagen [168], blended with chitosan [145] or blended with keratin [169]. Collagen-coated PHBV nanofibers alone or seeded with unrestricted somatic stem cells, isolated from umbilical cord, accelerated closure of excision wounds in rats in vivo compared to unmodified PHBV nanofibers [167].…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/collagen/graphene oxide scaffolds for wound coverage

Cited by 48 publications

References 24 publications

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Electrospinning of Collagen and Its Derivatives for Biomedical Applications

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

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