Nanofiber electrospinning combined with rotary bioprinting for fabricating small-diameter vessels with endothelium and smooth muscle

Jin, Qianheng; Fu, Yi; Zhang, Guangliang; Xu, Leyan; Jin, Guangzhe; Tang, Linfeng; Ju, Jihui; Zhao, Weixin; Hou, Ruixing

doi:10.1016/j.compositesb.2022.109691

Cited by 36 publications

(17 citation statements)

References 39 publications

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“…High-precision 3D printing permits the design of gelatin-based systems that mimic a tissue-like environment accurately and extends its use in complex applications, such as adipose, blood vessels, skeletal muscle or for wound dressing [28,[40][41][42]. For instance, in the search to find an alternative for adipose tissue regeneration, Daikuara et al designed a simple yet effective gelatin-based hydrogel using the bioprinting technique.…”

Section: Bioprintingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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Section: Bioprintingmentioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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“…The caplet form gives ease in the swallowing [ 40 ] 12 Ramipril Kollidon VA 64 Kollidon 12 PF Drug melting point is 109 0 C. No degradation observed due to use of proper combination. Choice of combination becomes important so as to overcome degradation problem due to thermal heating process [ 70 ] …”

Section: Main Bodymentioning

confidence: 99%

“…In advanced tissue engineering technology in the regeneration of blood vessels in addition to endothelial cells and muscle. Polycaprolactone (PCL) and methacrylate gelatin polymers mainly used in this case [ 70 ]. Method of electrospinning was used with polycaprolactone so as to improve adhesion property and elastic characteristics.…”

Section: Vascular Regenerationmentioning

confidence: 99%

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3D printing of pharmaceuticals: approach from bench scale to commercial development

Pawar

2022

Futur J Pharm Sci

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Background The three-dimensional (3D) printing is paradigm shift in the healthcare sector. 3D printing is platform technologies in which complex products are developed with less number of additives. The easy development process gives edge over the conventional methods. Every individual needs specific dose treatment. ‘One size fits all’ is the current traditional approach that can shift to more individual specific in 3D printing. The present review aims to cover different perspectives regarding selection of drug, polymer and technological aspects for 3D printing. With respect to clinical practice, regulatory issue and industrial potential are also discussed in this paper. Main body The individualization of medicines with patient centric dosage form will become reality in upcoming future. It provides individual’s need of dose by considering genetic profile, physiology and diseased condition. The tailormade dosages with unique drug loading and release profile of different geometrical shapes and sizes can easily deliver therapeutic dose. The technology can fulfill growing demand of efficiency in the dose accuracy for the patient oriented sectors like pediatric, geriatric and also easy to comply with cGMP requirements of regulated market. The clinical practice can focus on prescribing each individual’s necessity of dose. Conclusion In the year 2015, FDA approved first 3D printed drug product, which is initiator in the new phase of manufacturing of pharmaceuticals. The tailormade formulations can be made in future for personalized medications. Regulatory approval from agencies can bring the 3DP product into the market. In the future, formulators can bring different sector-specific products for personalized need through 3DP pharmaceutical product. Graphical Abstract

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“…Hence, regenerated blood vessels will be an appropriate solution for replacing these small diameter vessels (which were affected with secondary stenosis). Various techniques, such as extrusion, electrospinning, thermal-induced phase separation, braiding, hydrogel tubing, and gas foaming had been used for developing artificial blood vessels [ 2 , 3 , 4 , 5 ]. Serpentine vascular geometry having a rigid wall has been used in vascular regeneration [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Numerical and Experimental Analysis of Shear Stress Influence on Cellular Viability in Serpentine Vascular Channels

et al. 2022

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3D bioprinting has emerged as a tool for developing in vitro tissue models for studying disease progression and drug development. The objective of the current study was to evaluate the influence of flow driven shear stress on the viability of cultured cells inside the luminal wall of a serpentine network. Fluid–structure interaction was modeled using COMSOL Multiphysics for representing the elasticity of the serpentine wall. Experimental analysis of the serpentine model was performed on the basis of a desirable inlet flow boundary condition for which the most homogeneously distributed wall shear stress had been obtained from numerical study. A blend of Gelatin-methacryloyl (GelMA) and PEGDA200 PhotoInk was used as a bioink for printing the serpentine network, while facilitating cell growth within the pores of the gelatin substrate. Human umbilical vein endothelial cells were seeded into the channels of the network to simulate the blood vessels. A Live-Dead assay was performed over a period of 14 days to observe the cellular viability in the printed vascular channels. It was observed that cell viability increases when the seeded cells were exposed to the evenly distributed shear stresses at an input flow rate of 4.62 mm/min of the culture media, similar to that predicted in the numerical model with the same inlet boundary condition. It leads to recruitment of a large number of focal adhesion point nodes on cellular membrane, emphasizing the influence of such phenomena on promoting cellular morphologies.

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Nanofiber electrospinning combined with rotary bioprinting for fabricating small-diameter vessels with endothelium and smooth muscle

Cited by 36 publications

References 39 publications

Progress in Gelatin as Biomaterial for Tissue Engineering

Progress in Gelatin as Biomaterial for Tissue Engineering

3D printing of pharmaceuticals: approach from bench scale to commercial development

Numerical and Experimental Analysis of Shear Stress Influence on Cellular Viability in Serpentine Vascular Channels

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