Nanodrug-loaded Bifidobacterium bifidum conjugated with anti-death receptor antibody for tumor-targeted photodynamic and sonodynamic synergistic therapy

Li, Wenhao; Zhang, Zefei; Liu, Jie; Wang, Bo; Pu, Guangjin; Li, Ji; Huang, Yuqiao; Chu, Maoquan

doi:10.1016/j.actbio.2022.05.016

Cited by 11 publications

(13 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 Furthermore, ROS leads to damage of the mitochondrial membrane, which further leads to the release of cytochrome C (Cyt C) from mitochondria into cytoplasm and subsequently activates the caspasedependent apoptosis pathway to indirectly kill tumor cells. 30,31 Research results by Li et al 32 showed that US irradiation alone or chloride E6 (Ce6) alone showed no signicant anti-tumor effect on non-small cell lung carcinoma (NSCLC), but Ce6 combined with US irradiation had a signicant inhibitory effect on tumor growth. Flow cytometry analysis showed that Ce6 mediated the sonodynamic effect mainly through ROSinduced cell necrosis.…”

Section: The Cytotoxic Effects Of Sdtmentioning

confidence: 99%

Nanotechnology-enabled sonodynamic therapy against malignant tumors

Huang,

Ouyang,

Lai

et al. 2024

Nanoscale Adv.

View full text Add to dashboard Cite

show abstract

Section: The Cytotoxic Effects Of Sdtmentioning

confidence: 99%

Nanotechnology-enabled sonodynamic therapy against malignant tumors

Huang,

Ouyang,

Lai

et al. 2024

Nanoscale Adv.

View full text Add to dashboard Cite

show abstract

“…16−28 As the targeting of tumors by bacteria is not limited by tumor types, bacteria acting as drug carriers and/or therapeutic agents are suitable for treating nearly all tumors. Recently, bacteria, especially anaerobic and facultative bacteria, including E. coli 19,20 Salmonella, 21 Lactobacillus casei, 22 B. breve, 23 B. adolescentis, 24 Lactobacillus acidophilus, 27 B. Longum, 28 B. infantis, 29 Bifidobacterium bifidu (B. bifidum), 25,30 and magnetotactic bacteria, 26 have been investigated for their potential use for in vivo drug delivery and cancer therapy. Among these, magnetotactic bacteria, which is a type of aquatic prokaryote, may be excellent tumor-targeting agents due to their ability to actively swim and accumulate into tumors, following their direct tumoral targeting using an external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

“…infantis, Bifidobacterium bifidu (B. bifidum), , and magnetotactic bacteria, have been investigated for their potential use for in vivo drug delivery and cancer therapy. Among these, magnetotactic bacteria, which is a type of aquatic prokaryote, may be excellent tumor-targeting agents due to their ability to actively swim and accumulate into tumors, following their direct tumoral targeting using an external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Magnetotactic Bacteria-Based Drug-Loaded Micromotors for Highly Efficient Magnetic and Biological Double-Targeted Tumor Therapy

Wang

Qin

Liu

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Bacteria-mediated cancer therapy has attracted much attention in recent years. However, using magnetotactic bacteria as both a drug carrier and a drug for cancer therapy has never been reported. Herein, we incorporated a photosensitizer chlorin e6 (Ce6) into the M. magneticum strain AMB-1 through a chemical bond or physical blending. A chemical reaction was finally selected for fabricating AMB-1/Ce6 micromotors, as such micromotors exhibited high drug payload and normal bacterial activities. An interesting finding is that AMB-1 is not only an excellent drug carrier but also a unique drug that could inhibit mouse tumor growth. We also, for the first time, demonstrated that AMB-1 is a photosensitizer. Under laser irradiation, micromotors killed cancer cells with high efficiency due to the high-level reactive oxygen species generated by the micromotors. Micromotors could target the hypoxic and normoxic regions in vitro via both the active swimming of AMB-1 and external magnetic field guidance. Micromotors showed high tumor-homing ability owing to the above double targeting mechanisms. After injection with the micromotors followed by magnetic field guidance and laser irradiation, the growth of mouse tumors was significantly inhibited owing to the AMB-1-based biotherapy and phototoxicity of AMB-1 and Ce6. This micromotor-mediated tumor-targeted therapy strategy may be a great platform for treating many types of solid tumors.

show abstract

“…Towards this goal, a host of smart vectors have been designed, usually decorated with targeting moieties (antibodies, peptides etc.) [ 2 , 3 ] and often endowed with other capabilities, such as externally or site-activated release [ 4 , 5 ]. The progress in knowledge has been formidable, but delivery efficiencies remain very low, even for sophisticated multifunctional nanoparticles [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Exosomes loaded with ultrasmall Pt nanoparticles: a novel low-toxicity alternative to cisplatin

et al. 2022

View full text Add to dashboard Cite

Background Platinum nanoparticles have been demonstrated to have excellent anticancer properties. However, because of the lack of specificity they must be delivered to the tumor in amounts sufficient to reach the desired therapeutic objectives. Interestingly, exosomes are considered as excellent natural selective delivery nanotools, but until know their targeting properties have not being combined with the anticancer properties of platinum nanoparticles. Results In this work we combine the targeting capabilities of exosomes and the antitumoral properties of ultrasmall (< 2 nm) platinum nanoparticles as a novel, low toxicity alternative to the use of cisplatin. A mild methodology based on the room temperature CO-assisted in situ reduction of Pt2+ precursor was employed to preserve the integrity of exosomes, while generating ultrasmall therapeutic PtNPs directly inside the vesicles. The resulting PtNPs-loaded exosomes constitute a novel hybrid bioartificial system that was readily internalized by the target cells inducing antiproliferative response, as shown by flow cytometry and microscopy experiments in vitro. In vivo Pt-Exos showed antitumoral properties similar to that of cisplatin but with a strongly reduced or in some cases no toxic effect, highlighting the advantages of this approach and its potential for translation to the clinic. Conclusions In this study, a nanoscale vector based on ultrasmall PtNPs and exosomes has been created exhibiting antitumoral properties comparable or higher to those of the FDA approved cisplatin. The preferential uptake of PtNPs mediated by exosomal transfer between certain cell types has been exploited to create a selective antitumoral novel bioartificial system. We have demonstrated their anticancer properties both in vitro and in vivo comparing the results obtained with the administration of equivalent amounts of cisplatin, and showing a spectacular reduction of toxicity.

show abstract

Nanodrug-loaded Bifidobacterium bifidum conjugated with anti-death receptor antibody for tumor-targeted photodynamic and sonodynamic synergistic therapy

Cited by 11 publications

References 56 publications

Nanotechnology-enabled sonodynamic therapy against malignant tumors

Nanotechnology-enabled sonodynamic therapy against malignant tumors

Magnetotactic Bacteria-Based Drug-Loaded Micromotors for Highly Efficient Magnetic and Biological Double-Targeted Tumor Therapy

Exosomes loaded with ultrasmall Pt nanoparticles: a novel low-toxicity alternative to cisplatin

Contact Info

Product

Resources

About