Nanocrystalline diamond impedimetric aptasensor for the label-free detection of human IgE

Surface Modifications of Nanodiamonds and Current Issues for Their Biomedical Applications

“…Efficient surface treatments also permit to conjugate ND surfaces with fluorescent molecules [202][203][204][205], with DNA [206], siRNA [27], proteins [207,208], lysozymes [208], growth hormones [209], antibodies [210,211], anti-cancer drugs [30][31][32], as well as with dopamine derivatives [212].…”

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

“…Standard optical fibers have emerged over the years as excellent alternatives that can compete with the sensitivity and specificity of prism-based SPR sensors, while offering the advantage of low cost and small size greatly desired for developing a POC tool. One such platform, namely fiber optic surface plasmon resonance (FO-SPR), has been previously described by our group as a promising biosensor for protein-and DNA-based assays (Delport et al 2012;Janssen et al 2012;Knez et al 2013;Knez et al 2014;Pollet et al 2011;Pollet et al 2009;Tran et al 2011). The FO-SPR biosensor makes use of multimode optical fibers that facilitate SPR generation and enable implementation of bioassays.…”

Evolution towards the implementation of point-of-care biosensors

“…The selective antigen recognition was analyzed in real-time, and the detection limit was found to be 10 nM, which was in the physiologically relevant range, and could be discriminated within 10 minutes (Bijnens et al, 2009). Tran et al (2011) described an impedimetric aptasensor for the detection of human IgE. Human IgE has been demonstrated to be a mediator in allergic reactions.…”

“…IgE aptamers were covalently attached to NCD electrodes, and the impedimetric response was monitored continuously during IgE incubation. They obtained a detection limit of 0.03 µg/ml in serum (Tran et al, 2011).…”