Nanocomposite scaffolds composed of Apacite (apatite-calcite) nanostructures, poly (ε-caprolactone) and poly (2-hydroxyethylmethacrylate): The effect of nanostructures on physico-mechanical properties and osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro

Shams, Mehdi; Karimi, Mohammad; Heydari, Masoomeh; Salimi, Ali

doi:10.1016/j.msec.2020.111271

Cited by 11 publications

(3 citation statements)

References 57 publications

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“…Hydrophilic degradable porous 3D nanocomposite scaffolds composed of PCL, Poly (2hydroxyethylmethacrylate) (PHEMA), and Apacite (apatite-calcite) nanostructures (15 and 25 wt.%) with mechanical values (E~7.109 MPa and σ~0.414 MPa) provided a balanced microenvironment that resulted in osteogenic induction of human BMMSCs. Von Kossa staining, calcium content, and ALP results confirmed the highest bone cells' differentiation on PCLPHEMA/25% Apacite nanocomposites [196].…”

Section: Polyestersmentioning

confidence: 72%

Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

et al. 2021

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Mesenchymal stem/progenitor cells (MSCs) have a multi-differentiation potential into specialized cell types, with remarkable regenerative and therapeutic results. Several factors could trigger the differentiation of MSCs into specific lineages, among them the biophysical and chemical characteristics of the extracellular matrix (ECM), including its stiffness, composition, topography, and mechanical properties. MSCs can sense and assess the stiffness of extracellular substrates through the process of mechanotransduction. Through this process, the extracellular matrix can govern and direct MSCs’ lineage commitment through complex intracellular pathways. Hence, various biomimetic natural and synthetic polymeric matrices of tunable stiffness were developed and further investigated to mimic the MSCs’ native tissues. Customizing scaffold materials to mimic cells’ natural environment is of utmost importance during the process of tissue engineering. This review aims to highlight the regulatory role of matrix stiffness in directing the osteogenic differentiation of MSCs, addressing how MSCs sense and respond to their ECM, in addition to listing different polymeric biomaterials and methods used to alter their stiffness to dictate MSCs’ differentiation towards the osteogenic lineage.

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Section: Polyestersmentioning

confidence: 72%

Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

et al. 2021

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“…According to the definition, the viability of cells on the extracellular matrix is divided into four categories: severe toxicity (<30%), moderate toxicity (30%–60%), minor toxicity (60%–90%), and no toxicity (<90%). [ 82 ] According to this categorization and the attained results, the neat PU film and the nanocomposite loaded with hydrophobic graphene (PG5) exhibited minor toxicity since their cell viability values were obtained as 85.7 ± 1.1% and 83.9 ± 0.8%, respectively. All the other samples, including PG*5, PE10G5, and PE10G*5, have shown less toxicity with cell viability values of 92.4 ± 1%, 90.2 ± 0.9%, and 98.1 ± 0.5%, respectively, and thus regarded as non‐toxic.…”

Section: Resultsmentioning

confidence: 97%

Surface modification of polyurethane nanocomposite films via nonsolvent‐induced phase separation accelerated by graphene nanoplatelets

et al. 2022

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Polyurethane (PU) nanocomposite films were prepared through a solution casting technique slightly modified by individual and simultaneous additions of a proper nonsolvent (ethanol) and graphene nanoplatelets. To this end, different nonsolvent contents and graphene functionalizations (hydrophobic and hydrophilic) were used to modify the surface properties of PU to make it more biocompatible. X-ray diffraction patterns revealed that both types of graphene had a hampering effect on crystallization of PU from solution, while nonsolvent enhanced the PU crystallinity. According to morphological results, hydrophobic graphene showed a low dispersion quality. In contrast, the hydrophilic graphene was better dispersed and mainly localized at the bulk of the film due to its association with polar urethane groups in the hard segments. Wettability analysis, performed by water and diiodomethane, was employed to demonstrate a higher surface localization of hard segments for the samples loaded with hydrophilic graphene. Cytotoxicity analysis also showed a greater extent of cell viability for the samples loaded with hydrophilic graphene, demonstrating a direct correlation between biocompatibility and polar hydrophilic groups enriching the surface. This study shows the great potential of graphene in improving the nonsolvent-induced phase separation to achieve a more biocompatible material.

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“…As observed from SEM investigations, the laser fluence did not alter the uniform distribution of small aggregates of PLA/HAp/BMP4 composite material onto the substrate. The continuous sub-micron coating (Figure 5c) was composed of particulates of different sizes, arbitrary scattered on the surface, which have a positive outcome on cell adhesion and growth/proliferation [88][89][90].…”

Section: Physicochemical Characterization Of Pla/hap/bmp4 Coatingsmentioning

confidence: 99%

Bioactive Coatings Loaded with Osteogenic Protein for Metallic Implants

Gherasim

Grumezescu

et al. 2021

Polymers

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Osteoconductive and osteoinductive coatings represent attractive and tunable strategies towards the enhanced biomechanics and osseointegration of metallic implants, providing accurate local modulation of bone-to-implant interface. Composite materials based on polylactide (PLA) and hydroxyapatite (HAp) are proved beneficial substrates for the modulation of bone cells’ development, being suitable mechanical supports for the repair and regeneration of bone tissue. Moreover, the addition of osteogenic proteins represents the next step towards the fabrication of advanced biomaterials for hard tissue engineering applications, as their regulatory mechanisms beneficially contribute to the new bone formation. In this respect, laser-processed composites, based on PLA, Hap, and bone morphogenetic protein 4(BMP4), are herein proposed as bioactive coatings for metallic implants. The nanostructured coatings proved superior ability to promote the adhesion, viability, and proliferation of osteoprogenitor cells, without affecting their normal development and further sustaining the osteogenic differentiation of the cells. Our results are complementary to previous studies regarding the successful use of chemically BMP-modified biomaterials in orthopedic and orthodontic applications.

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Nanocomposite scaffolds composed of Apacite (apatite-calcite) nanostructures, poly (ε-caprolactone) and poly (2-hydroxyethylmethacrylate): The effect of nanostructures on physico-mechanical properties and osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro

Cited by 11 publications

References 57 publications

Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

Surface modification of polyurethane nanocomposite films via nonsolvent‐induced phase separation accelerated by graphene nanoplatelets

Bioactive Coatings Loaded with Osteogenic Protein for Metallic Implants

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