Nanocoating for biomolecule delivery using layer-by-layer self-assembly

Understanding the relationship between liquid manipulation and micro‐/nanostructured interfaces has gained much attention due to the wide potential applications in many fields, such as chemical and biomedical assays, environmental protection, industry, and even daily life. Much work has been done to construct various materials with interfacial liquid manipulation abilities, leading to a range of interesting applications. Herein, different fabrication methods from the top‐down approach to the bottom‐up approach and subsequent surface modifications of micro‐/nanostructured interfaces are first introduced. Then, interactions between the surface and liquid, including liquid wetting, liquid transportation, and a number of corresponding models, together with the definition of hydrophilic/hydrophobic, oleophilic/olephobic, the definition and mechanism of superwetting, including superhydrophobicity, superhydrophilicity, and superoleophobicity, are presented. The micro‐/nanostructured interface, with major applications in self‐cleaning, antifogging, anti‐icing, anticorrosion, drag‐reduction, oil–water separation, water collection, droplet (micro)array, and surface‐directed liquid transport, is summarized, and the mechanisms underlying each application are discussed. Finally, the remaining challenges and future perspectives in this area are included.

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Section: Fabrication Of Micro‐/nanostructurementioning

confidence: 99%

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Duan

Zheng

Zhao

et al. 2019

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“…Our system prevents the dissociation of FA from the surface by covalently linking it to the DAS. Furthermore, the lumen of HNTs makes them a particularly interesting nanocarrier for the loading and release of various biologically active molecules, including small molecules, enzymes, and proteins including chemotherapeutic payloads [54][55][56][57][58][59][60][61]. We envision our construct as a potential modular platform for use in anti-cancer therapies.…”

Section: Cytotoxicity Testing Of Bhntsmentioning

confidence: 99%

Bi-Functionalized Clay Nanotubes for Anti-Cancer Therapy

et al. 2018

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Systemic toxicity is an undesired consequence of the majority of chemotherapeutic drugs. Multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise towards personalized nanomedicine. Halloysite clay nanotubes (HNTs) have shown potential as a drug delivery vehicle, and its surface can be modified and tailored as a targeted drug delivery system. In this short report, we modified the HNT surface by covalently bonding folic acid (FA) and fluorescein isothiocyanate (FITC). The modification of HNTs with folic acid imparts the potential to target tumor cells selectively. The addition of FITC offers a method for quantifying the effectiveness of the FA tagged HNTs ability to target tumor cells. We documented cell uptake of our bi-functionalized HNT (bHNT) through phase contrast and epi-fluorescent microscopy. bHNTs showed no signs of cytotoxicity up to a concentration of 150 µg/mL. The increase in cell death with increased bHNT concentration may be due to induced cytotoxicity resulting from intracellular bHNT accumulation that disrupts cellular function leading to cell death. With HNTs recognized as having the ability to serve as both a nanocontainer and nanocarrier, we envision our construct as a potential modular platform for potential use in cancer therapeutics. The HNT interior can be loaded with a variety of anti-cancer drugs (or other chemotherapeutics) and serve as a "death cargo" designed to kill cancer cells while providing feedback imaging data on drug efficacy. The surface of the HNT can be modified with gold or silver nanoparticles and used in photothermal therapy by converting light to heat inside tumors. Our HNT-based drug delivery system has the potential to provide localized and targeted therapies that limit or reduce side effects, reduce patient costs and length of hospital stays, and improve quality of life. However, further research is needed to validate the potential of this new chemotherapeutic drug delivery system.

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“…According to the source of the materials, the polyelectrolyte materials are classied into different categories, including synthetic and natural type polyelectrolytes. 8 Herewith, we designed the shell LbL assembly using two functional polymers i.e., poly-L-ornithine (PLO) and fucoidan. PLO (Fig.…”

Section: -7mentioning

confidence: 99%

Synthesis and characterization of innovative poly(lactide-co-glycolide)-(poly-l-ornithine/fucoidan) core–shell nanocarriers by layer-by-layer self-assembly

et al. 2017

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Layer-by-Layer (LbL) self-assembly of nanocarriers has garnered the interest of researchers for a wide variety of biomedical applications. In this study, we demonstrated the preparation of poly(lactide-coglycolide) (PLGA)-(poly-L-ornithine (PLO)/fucoidan) 4 core-shell nanoparticles (LbL NPs) by a LbL-based self-assembly process, which possessed a mean size of 170 nm. In LbL NPs, a drug carrying PLGA nanocore is coated with alternating PLO and sulfated polysaccharide fucoidan composite films as a shell on the surface. The anti-tumor drug doxorubicin (DOX) loaded into the PLGA core, resulted in better encapsulation efficiency and its in vitro release from LbL NPs demonstrates that this core-shell strategy takes an advantage of its ability to hold the drug cargo and exhibit controlled release. Further, in vitro cell uptake studies by confocal laser scanning microscopy (CLSM) examination in breast tumor cells (MCF-7 cell line) have confirmed that the nanocarriers are successfully internalized and outlined their presence in the cytoplasm after 4 h of incubation. These intracellularly delivered DOX-loaded LbL NPs exhibited significant anti-tumor activity against breast tumor cells. This innovative chemotherapeutic design taking above advantages of successful internalization along with controlled release property signifies as a promising interventional therapeutic delivery system.

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Nanocoating for biomolecule delivery using layer-by-layer self-assembly

Cited by 108 publications

References 113 publications

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Micro‐/Nanostructured Interface for Liquid Manipulation and Its Applications

Bi-Functionalized Clay Nanotubes for Anti-Cancer Therapy

Synthesis and characterization of innovative poly(lactide-co-glycolide)-(poly-l-ornithine/fucoidan) core–shell nanocarriers by layer-by-layer self-assembly

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