Synthesis and characterization of innovative poly(lactide-co-glycolide)-(poly-l-ornithine/fucoidan) core–shell nanocarriers by layer-by-layer self-assembly

Abstract

Layer-by-Layer (LbL) self-assembly of nanocarriers has garnered the interest of researchers for a wide variety of biomedical applications. In this study, we demonstrated the preparation of poly(lactide-coglycolide) (PLGA)-(poly-L-ornithine (PLO)/fucoidan) 4 core-shell nanoparticles (LbL NPs) by a LbL-based self-assembly process, which possessed a mean size of 170 nm. In LbL NPs, a drug carrying PLGA nanocore is coated with alternating PLO and sulfated polysaccharide fucoidan composite films as a shell on the surface. The anti-tumor drug doxorubicin (DOX) loaded into the PLGA core, resulted in better encapsulation efficiency and its in vitro release from LbL NPs demonstrates that this core-shell strategy takes an advantage of its ability to hold the drug cargo and exhibit controlled release. Further, in vitro cell uptake studies by confocal laser scanning microscopy (CLSM) examination in breast tumor cells (MCF-7 cell line) have confirmed that the nanocarriers are successfully internalized and outlined their presence in the cytoplasm after 4 h of incubation. These intracellularly delivered DOX-loaded LbL NPs exhibited significant anti-tumor activity against breast tumor cells. This innovative chemotherapeutic design taking above advantages of successful internalization along with controlled release property signifies as a promising interventional therapeutic delivery system.

Primary biocompatibility tests of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) core–shell nanocarriers

“…LbL self-assembled NPs were prepared as previously described [ 14 ]. Briefly, the PLGA NPs were prepared by the O/W emulsion–solvent evaporation method, and then PLO and fucoidan were deposited layer by layer alternately on the surface of the substrate core by electrostatic interaction.…”

“…As a potential new drug carrier, poly(lactide-co-glycolide) [PLGA]-(PLO/fucoidan) core–shell nanocarriers should not only possess drug-loading and release properties [ 14 ], but also good biocompatibility. Herein, the surface morphology and residual organic solvents were assessed.…”

Poly‐allylamine hydrochloride and fucoidan‐based self‐assembled polyelectrolyte complex nanoparticles for cancer therapeutics

“…In addition to synthetic polymers, various natural polysaccharides have received extensive attention in the fabrication of drug delivery systems, especially nanocarriers, due to tunable electronic architecture and tunable physicochemical attributes. Various polymers include chitosan, alginic acid, cyclodextrin, and fucoidan . The development of drug delivery systems with diversified functions remains as an important direction for the study of functional materials.…”

“…Various polymers include chitosan, 4,5 alginic acid, 6,7 cyclodextrin, [8][9][10] and fucoidan. [11][12][13][14] The development of drug delivery systems with diversified functions remains as an important direction for the study of functional materials. In this vein, fucoidan, a natural polysaccharide, is an interesting candidate for biomedical applications because of its promising properties such as biocompatibility and ease of encapsulation of bioactive molecules and less immunogenicity, among others.…”

Bacterial magnetosomes as an efficient gene delivery platform for cancer theranostics

“…Pharmaceutical carriers often use polymers, dendrimers, micelles, liposomes, inorganic nanomaterials and so on [ 15 – 21 ], which can all be employed for drug delivery system. Amongst inorganic nanomaterials, bacterial magnetosomes (BMs) have shown a great potential as a novel carrier due to their excellent biocompatibility, high surface area to volume ratio, superparamagnetism and abundant active sites on the membrane of BMs [ 22 ].…”