Bi-Functionalized Clay Nanotubes for Anti-Cancer Therapy

Grimes, W.R.; Luo, Yangyang; McFarland, Antwine W.; Mills, David K.

doi:10.3390/app8020281

Cited by 20 publications

(14 citation statements)

References 60 publications

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“…In our previous study [ 16 ], we assessed bi-HNTs cytotoxicity and observed that cytotoxicity increased with bi-HNT concentration. In order to confirm the optimal dosage range for bi-HNTs, we used an MTS assay and XTT assay to assess cell proliferation and viability with cells co-cultured with pure HNTs and bi-HNTs at different concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…bi-HNTs were synthesized using the same methods as described in a previous study ( Figure 2 ) [ 16 ]. Briefly, HNTs (Sigma Aldrich, St. Louis, MO, USA) were reacted at reflux with N -[3-(trimethoxysilyl) propyl] ethylenediamine (DAS) (Sigma Aldrich, St. Louis, MO., USA) in toluene for 24 h. Then, the HNT-DAS composite was reacted with FA (Sigma Aldrich, St. Louis, MO, USA) in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Sigma Aldrich, St. Louis, MO, USA) in DI water overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Surface modification using FA and FITC was further confirmed by FTIR, 13C CPMAS NMR spectrum, and UV-Vis. Folic acid directed HNTs adhered to folate receptors, which are overexpressed in numerous cancers but rarely expressed or nonexistent in most normal tissues [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Cellular Analysis and Chemotherapeutic Potential of a Bi-Functionalized Halloysite Nanotube

et al. 2020

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The surface of halloysite nanotubes (HNTs) was bifunctionalized with two ligands—folic acid and a fluorochrome. In tandem, this combination should selectively target cancer cells and provide a means for imaging the nanoparticle. Modified bi-functionalized HNTs (bi-HNTs) were then doped with the anti-cancer drug methotrexate. bi-HNTs were characterized and subjected to in vitro tests to assess cellular growth and changes in cellular behavior in three cell lines—colon cancer, osteosarcoma, and a pre-osteoblast cell line (MC3T3-E1). Cell viability, proliferation, and cell uptake efficiency were assessed. The bi-HNTs showed cytocompatibility at a wide range of concentrations. Compared with regular-sized HNTs, reduced HNTs (~6 microns) were taken up by cells in more significant amounts, but increased cytotoxicity lead to apoptosis. Multi-photon images confirmed the intracellular location of bi-HNTs, and the method of cell entry was mainly through caveolae-mediated endocytosis. The bi-HNTs showed a high drug loading efficiency with methotrexate and a prolonged period of release. Most importantly, bi-HNTs were designed as a drug carrier to target cancer cells specifically, and imaging data shows that non-cancerous cells were unaffected after exposure to MTX-doped bi-HNTs. All data provide support for our nanoparticle design as a mechanism to selectively target cancer cells and significantly reduce the side-effects caused by off-targeting of anti-cancer drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cellular Analysis and Chemotherapeutic Potential of a Bi-Functionalized Halloysite Nanotube

et al. 2020

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“…The hollow tubular shape and peculiar surface properties make HNTs suitable as catalytic supports for several reactions [29][30][31][32][33][34] as well as adsorbent systems for wastewater decontamination [35][36][37][38]. Filling polymeric matrices with halloysite presents as a successful strategy to obtain functional nanocomposite materials with excellent characteristics in terms of mechanical performance [39,40], thermal stability [10,41,42], and vapor barrier properties [43].The unique behavior of HNTs allows for selective modification with functional molecules; promising for the preparation of organic/inorganic composites [44,45]. The opposite charges of the inside and outside surfaces allow for the selective adsorption of anionic or cationic surfactants presenting as an easy strategy to prepare new advanced hybrid materials [46,47].…”

mentioning

confidence: 99%

“…The unique behavior of HNTs allows for selective modification with functional molecules; promising for the preparation of organic/inorganic composites [44,45]. The opposite charges of the inside and outside surfaces allow for the selective adsorption of anionic or cationic surfactants presenting as an easy strategy to prepare new advanced hybrid materials [46,47].…”

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confidence: 99%

Microemulsion Encapsulated into Halloysite Nanotubes and their Applications for Cleaning of a Marble Surface

et al. 2018

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Featured Application: Halloysite nanotubes for surface cleaning. Controlled cleaning of a marble surface.Abstract: Halloysite nanotubes were used to incorporate anionic surfactant micelles and an organic solvent to generate a cleaning system to be applied in Cultural Heritage restoration. The targeted adsorption is driven by electrostatic interactions based on the nanotubes peculiar charge separation. Namely anionic species are driven to the positively charged inner surface while being prevented from interacting with the halloysite outer surface that possesses a positive charge density. The hybrid organic/inorganic emulsion was characterized by dynamic light scattering. Analysis of the autocorrelation function allowed us to define the presence of surfactant aggregates inside/outside the nanotube lumen as a function of the nanotube/surfactant ratio in an aqueous mixture. The application of this prepared emulsion for the controlled cleaning of a marble artifact is demonstrated. To this purpose, a membrane of nanofibrous polyacrylonitrile was prepared by electrospinning and was applied between the work of art and the cleaning agent to avoid the release of residues on the marble surface. This work represents a further step toward the opportunity to extend the use of emulsions for cleaning protocols for stone-based artifacts or in technological applications where surfactant separation is required by a simple centrifugation/sedimentation method. 2 of 10 with a significant reduction in toxicity and environmental impact. Restoration of the Renaissance paintings by Masaccio, Masolino, and Lippi in the Brancacci Chapel in Florence (1984)(1985)(1986)(1987)(1988)(1989)(1990) represents the first case study reporting the use of microemulsions for conservation purposes [2,3]. Recently, amphiphile-based systems were intensively studied by Piero Baglioni to remove synthetic materials (acrylic and vinyl polymers) from painted surfaces [4]. These results show that the designed nanostructures improved the traditional methods while both minimizing the environmental impact and the optimizing cleaning performance [4]. Disadvantages for the use of colloidal systems are related to the high wettability of the treated surface and to spreading within the substrate porosity. Small traces of surfactant that remain on the artwork may cause deterioration and, in some cases, lead to the complete disfiguration of painted surfaces [5]. A fascinating resolution is freezing the microemulsion dynamics into a rigid nanocontainer in such a way as to simplify the removal of the whole cleaning system [6].Among nanomaterials, hybrid systems prepared by a combination of mesoporous particles and sustainable polymers or surfactants are promising for several applications within materials science and biotechnology [1,[7][8][9][10][11][12][13][14][15]. Clay nanoparticles are promising as solid supports due to their sustainability and low cost [16][17][18][19][20]. Halloysite is an emerging hollow tubular clay which is naturally available worldwide and cheap co...

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