Nanobiofungicides: is it the next-generation of fungicides?

Abd‐Elsalam, Kamel A.

doi:10.15436/2377-1372.15.013

Cited by 20 publications

(13 citation statements)

References 25 publications

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“…In order to obtain higher crop production yields, it is necessary to develop new plant varieties by introducing traits that ideally enables them to better resist different environmental-derived abiotic stresses or pathogen-mediated diseases along with the generation of higher biomass under limited resources. The transfer of genes to the target plant cells is challenging due to the rigid plant cell wall which prevents the exogenous particle movement from the outside to the cytoplasm (Abd-Elsalam and Alghuthaymi, 2015). There is evidence that the plant's nanoparticle uptake is strongly dependent on the cell wall pore diameter (i.e., exclusion size limit), which may vary amongst different tissues and organs.…”

Section: Nano-carriers As a Non-viral Vector For Gene Delivery In Plamentioning

confidence: 99%

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Vega-Vásquez

Mosier

Irudayaraj

2020

Front. Bioeng. Biotechnol.

197

107

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The main challenges in drug delivery systems are to protect, transport and release biologically active compounds at the right time in a safe and reproducible manner, usually at a specific target site. In the past, drug nano-carriers have contributed to the development of precision medicine and to a lesser extent have focused on its inroads in agriculture. The concept of engineered nano-carriers may be a promising route to address confounding challenges in agriculture that could perhaps lead to an increase in crop production while reducing the environmental impact associated with crop protection and food production. The main objective of this review is to contrast the advantages and disadvantages of different types of nanoparticles and nano-carriers currently used in the biomedical field along with their fabrication methods to discuss the potential use of these technologies at a larger scale in agriculture. Here we explain what is the problem that nano-delivery systems intent to solve as a technological platform and describe the benefits this technology has brought to medicine. Also here we highlight the potential drawbacks that this technology may face during its translation to agricultural applications, based on the lessons learned so far from its use for biomedical purposes. We discuss not only the characteristics of an ideal nano-delivery system, but also the potential constraints regarding the fabrication including technical, environmental, and legal aspects. A key motivation is to evaluate the potential use of these systems in agriculture, especially in the area of plant breeding, growth promotion, disease control, and post-harvest quality control. Further, we highlight the importance of a rational design of nano-carriers and identify current research gaps to enable scale-up relevant to applications in the treatment of plant diseases, controlled release of fertilizers, and plant breeding.

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Section: Nano-carriers As a Non-viral Vector For Gene Delivery In Plamentioning

confidence: 99%

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Vega-Vásquez

Mosier

Irudayaraj

2020

Front. Bioeng. Biotechnol.

197

107

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“…Nevertheless, the same problems with their usage exist. To enhance antifungal activity and decrease environmental toxic effect, it is important to develop novel type of biocides [1,2].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

et al. 2017

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In this report, the metal-vapor synthesis (MVS) was used for the preparation of copper nanoparticles which was then used for the preparation of chitosan-copper nanocomposite. The antifungal activity of Cu@Chit NCs against two sclerotium-forming plant pathogenic fungi Sclerotium rolfsii (S. rolfsii) and Rhizoctonia solani (R. solani) AG-4 was evaluated in vitro and their effects on hyphal morphology, and sclerotia formation were observed for the first time. The NCs were prepared through impregnation of chitosan with colloid solution of copper nanoparticles in organic solvent (acetone or toluene). Transmission electron microscopy shows that the particles have predominantly spherical form, polydisperse character, the mean diameter about 2-3 nm and a rather uniform distribution in the chitosan matrice. Analysis of the small angle scattering curves suggests that the copper particles in the NCs with the size of B2 nm are mostly located in the chitosan pores with the same size. The effect of Cu@Chit NCs on fungal growth reveals some significant inhibitory activity against two tested fungi. The highest level of inhibition against S. rolfsii and R. solani AG-4 was observed using the high concentrations of Cu@Chit NC prepared using acetone as a solvent. A loss of the cytoplasm content, cytoplasmic coagulation, irregular shape of mycelia, or destruction in the hyphae was confirmed. The experiments demonstrate that the Cu@Chit NC synthesized via MVS using acetone was more effective than that of toluene in inhibiting fungal hyphae growth against R. solani AG-4 and S. rolfsii. The results show that the Cu@Chit NCs are fungicidal against both the tested fungus at high concentrations and the fungicidal or fungistatic activity is dependent on the tested fungus species.

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“…During the past decade, many biological systems (bacteria, yeast, and fungi) has been demonstrated and developed for biosynthesis of nano-metal oxide by using extracted proteins and metabolites 23 – 25 , 61 . For industrial production of nano-metal oxide; the safety, the low cost of microbial cultivation, short biosynthesis reaction time, and the ability to scale-up production; make Streptomyces sp ., an attractive factory for a biogenic synthesis of nanoparticles that used for therapeutic applications 17 , 50 , 60 , 62 . Recently, biogenic synthesis of nanoparticles has taken an important stage in the modern industrial pharmaceutical sector, so exploring novel eco-friendly bioactive metabolites extracted from safe potential bio-factories that possess low toxicity was studied in this work.…”

Section: Discussionmentioning

confidence: 99%

“…The filtrates were then evaporated using a rotary evaporator at 40 °C. The crude intracellular bioactive metabolite was extracted from the washed biomass (the pellet) that treated with CTAB extraction buffer (100 mM Tris-Cl (pH 8.0), 20 mM EDTA (pH 8.0), 1.4 M NaCl, 2% (w/v) cetyltrimethyl ammonium bromide and 1% PVP 40,000 (polyvinyl pyrrolidone) for 2 hr under shaking condition and finally centrifuged at 14000 rpm for 10 min 44 , 50 , 51 . The concentrated bioactive metabolites extracts were then transferred into clean glass screw cap tube and stored at 4 °C for further use.…”

Section: Methodsmentioning

confidence: 99%

“…Nutrient agar plates were prepared and swabbed with 100 μl of mature broth culture of human pathogenic bacterial strains individually. Firstly, the pathogens sensitivity was tested by using the standard antibiotics 58 (Amikacin 30 mcg, Ampicilin/sulbactam 40 μg, Ampicillin 10 μg, Cefotaxime 30 µg, Cefoxitin 30 µg, Cephradine 30 μg, Chloramphenicol 30 mcg, Erythromycin 15 mcg, Gentamicin 10 mcg, Nitrofurantion 300 mcg, Norfloxacin 10 mcg, Pefloxacin 5 mcg and Penicillin G 10 U) through disc diffusion method 23 – 25 , 43 , 50 , 59 , 60 . Secondly, the minimum inhibitory concentration (a concentration which completely inhibited growth) were calculated by using different concentration of pure nano-MgO solution (5, 10, 15, 20, 25, 30, 35, 40, 45, 50 and 55 µg/ml) through well diffusion method.…”

Section: Methodsmentioning

confidence: 99%

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Bioprocessing strategies for cost-effective large-scale biogenic synthesis of nano-MgO from endophytic Streptomyces coelicolor strain E72 as an anti-multidrug-resistant pathogens agent

EL-Moslamy

2018

Sci Rep

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In this report, the local nano-MgO synthesizer strain has been isolated from Ocimum sanctum plant and deposited in GenBank as endophytic Streptomyces coelicolor strain E72. Its intracellular metabolic fraction that contains 7.2 μg/μl of carbohydrate, 6.3 g/l of protein and 5.2 nmol/hr/ml of nitrate reductase used to produce multi-surface shaped nano-MgO with diameter ~25 nm. To the best of our knowledge, this is the first report using statistical nanobiotechnological strategies (Plackett -Burman, Box-Behnken and Taguchi experimental designs) to study and evaluate the endophytic S. coelicolor biomass production (123.3 g/l) and extract the highest bioactive metabolites that used for biogenic synthesis of nano-MgO (320 g/l) through exponential sucrose pulses feeding fermentation strategy after 192 hr in semi industrial scale bioreactor (7 L). Purified nano-MgO applied in vitro against multi-drug resistant human pathogens and the large inhibition zone recorded against Shigella flexneri (108 ± 10.53 mm). The average of MICs was recorded as 25 µg/ml that inhibited 90% of the pathogenic living cells and compared with 100 mg/ml ampicilin/sulbactam solution that killed 40% of the same pathogen. These results are expected to gather sufficient knowledge to discover and develop a new cheap and eco-friendly nano-MgO as an extremely strong antimicrobial agent used in biomedical applications.

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Nanobiofungicides: is it the next-generation of fungicides?

Cited by 20 publications

References 25 publications

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Synthesis and characterization of chitosan–copper nanocomposites and their fungicidal activity against two sclerotia-forming plant pathogenic fungi

Bioprocessing strategies for cost-effective large-scale biogenic synthesis of nano-MgO from endophytic Streptomyces coelicolor strain E72 as an anti-multidrug-resistant pathogens agent

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