Bioprocessing strategies for cost-effective large-scale biogenic synthesis of nano-MgO from endophytic Streptomyces coelicolor strain E72 as an anti-multidrug-resistant pathogens agent

Abstract

In this report, the local nano-MgO synthesizer strain has been isolated from Ocimum sanctum plant and deposited in GenBank as endophytic Streptomyces coelicolor strain E72. Its intracellular metabolic fraction that contains 7.2 μg/μl of carbohydrate, 6.3 g/l of protein and 5.2 nmol/hr/ml of nitrate reductase used to produce multi-surface shaped nano-MgO with diameter ~25 nm. To the best of our knowledge, this is the first report using statistical nanobiotechnological strategies (Plackett -Burman, Box-Behnken and Taguchi experimental designs) to study and evaluate the endophytic S. coelicolor biomass production (123.3 g/l) and extract the highest bioactive metabolites that used for biogenic synthesis of nano-MgO (320 g/l) through exponential sucrose pulses feeding fermentation strategy after 192 hr in semi industrial scale bioreactor (7 L). Purified nano-MgO applied in vitro against multi-drug resistant human pathogens and the large inhibition zone recorded against Shigella flexneri (108 ± 10.53 mm). The average of MICs was recorded as 25 µg/ml that inhibited 90% of the pathogenic living cells and compared with 100 mg/ml ampicilin/sulbactam solution that killed 40% of the same pathogen. These results are expected to gather sufficient knowledge to discover and develop a new cheap and eco-friendly nano-MgO as an extremely strong antimicrobial agent used in biomedical applications.

Effective Inhibition of Candidiasis Using an Eco-Friendly Leaf Extract of Calotropis-gigantean-Mediated Silver Nanoparticles

“…In this context, the antimicrobial action of bio-factorized AgNPs with promising therapeutic effects was reported in many studies [8,9]. The green synthesis of nanoparticles by biological methods using plants has shown numerous advantages, including low costs and the large-scale production of nanoparticles, as well as being environmentally safe [10]. On the other hand, nanoparticle production using physical methods demands high energy consumption and chemical methods produce hazardous and toxic compounds that reduce the biological applications of generated nanoparticles [11].…”

Endophytic Nanotechnology: An Approach to Study Scope and Potential Applications

“…Exserohilum rostrata has been used to synthesize Ag NPs (size ranges from 15 to 45 nm) for their antioxidant and anti-inflammatory activities (Bagur et al, 2019). Actinomycete Streptomyces are known to produce a broad range of secondary metabolites and can be utilized for the clinical use as antifungals, antibiotics, anticancer, immunosuppressives, antivirals and insecticides (Messaoudi et al, 2015;El-Gamal et al, 2018;El-Moslamy et al, 2018;Singh et al, 2019). Streptomyces capillispiralis and Streptomyces zaomyceticus Oc-5 have been used for the synthesis of Cu NPs (Hassan et al, 2018;Hassan et al, 2019).…”

Biological Synthesis of Nanoparticles Using Endophytic Microorganisms: Current Development

“…Nanoparticles from endophytic actinobacteria is an emerging field yet to be established, in fact, when compared with fungi and the other bacteria, only few publications have been reported. Most of the articles about nanoparticles from endophytic actinomycetes, reporting the synthesis of nanoparticles using endophytes belong to the genus of Streptomyces, however, nanoparticles synthesized by rare actinobacteria have been reported in a few papers [60,62].…”