Nano‐vesicular formulation of propolis and cytotoxic effects in a <scp>3D</scp> spheroid model of lung cancer

Ilhan‐Ayisigi, Esra; Ulucan, Fulden; Saygili, Ecem; Sağlam-Metiner, Pelin; Gülce-İz, Sultan; Yeşil-Çeliktaş, Özlem

doi:10.1002/jsfa.10400

Cited by 28 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ilhan-Ayisigi et al synthesized niosome nanovesicles doped with a galangin-rich propolis extract. These nanoparticles demonstrated a profound cytotoxic effect on the A549 spheroid model ( 100 ).…”

Section: Nsclc Spheroids For the Evaluation Of Novel Drug Candidates And Nanomedicinesmentioning

confidence: 99%

Biomedical Applications of Non-Small Cell Lung Cancer Spheroids

et al. 2021

View full text Add to dashboard Cite

Lung malignancies accounted for 11% of cancers worldwide in 2020 and remained the leading cause of cancer deaths. About 80% of lung cancers belong to non-small cell lung cancer (NSCLC), which is characterized by extremely high clonal and morphological heterogeneity of tumors and development of multidrug resistance. The improvement of current therapeutic strategies includes several directions. First, increasing knowledge in cancer biology results in better understanding of the mechanisms underlying malignant transformation, alterations in signal transduction, and crosstalk between cancer cells and the tumor microenvironment, including immune cells. In turn, it leads to the discovery of important molecular targets in cancer development, which might be affected pharmaceutically. The second direction focuses on the screening of novel drug candidates, synthetic or from natural sources. Finally, “personalization” of a therapeutic strategy enables maximal damage to the tumor of a patient. The personalization of treatment can be based on the drug screening performed using patient-derived tumor xenografts or in vitro patient-derived cell models. 3D multicellular cancer spheroids, generated from cancer cell lines or tumor-isolated cells, seem to be a helpful tool for the improvement of current NSCLC therapies. Spheroids are used as a tumor-mimicking in vitro model for screening of novel drugs, analysis of intercellular interactions, and oncogenic cell signaling. Moreover, several studies with tumor-derived spheroids suggest this model for the choice of “personalized” therapy. Here we aim to give an overview of the different applications of NSCLC spheroids and discuss the potential contribution of the spheroid model to the development of anticancer strategies.

show abstract

Section: Nsclc Spheroids For the Evaluation Of Novel Drug Candidates And Nanomedicinesmentioning

confidence: 99%

Biomedical Applications of Non-Small Cell Lung Cancer Spheroids

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Cells were cultured at 37°C in a humidified atmosphere containing 5% CO 2 . MCTS were generated as described previously (Ilhan-Ayisigi et al, 2020), with slight modifications. The MicroTissues ® 3D Petri Dish ® micro-mold spheroids, size L, 5 × 7 array (Sigma-Aldrich) was used to generate MCTS.…”

Section: Cell Culture and Spheroid Formationmentioning

confidence: 99%

The matrix-dependent 3D spheroid model of the migration of non-small cell lung cancer: a step towards a rapid automated screening

Shabalina

Skorova

Chudakova

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

In vitro 3D cell culture systems utilizing multicellular tumor spheroids (MCTS) are widely used in translational oncology, including for studying cell migration and in personalized therapy. However, early stages of cellular migration from MCTS and cross-talk between spheroids are overlooked, which was addressed in the current study. Here, we investigated cell migration from MCTS derived from human non-small cell lung cancer (NSCLC) cell line A549 cultured on different substrates, collagen gel or plastic, at different time points. We found that migration starts at 4–16 h time points after the seeding and its speed is substrate-dependent. We also demonstrated that co-culture of two NSCLC-derived MCTS on collagen gel, but not on plastic, facilitates cell migration compared with single MTCS. This finding should be considered when designing MCTS-based functional assays for personalized therapeutic approach and drug screenings. Overall, our work characterizes the in vitro 3D cell culture model resembling NSCLC cell migration from the clusters of CTCs into surgical wound, and describes microscopy-based tools and approaches for image data analysis with a potential for further automation. These tools and approaches also might be used to predict patterns of CTCs migration based on ex vivo analysis of patient biopsy in a 3D culture system.

show abstract

“…The lung cancer cells treated with propolis nanosized showed a significant decrease in cell survival and scattering, revealing a significant cytotoxic impact and might be used as an efficient nano-vesicle (Ilhan-Ayisigi et al, 2020). The stingless bee propolis (Tetragonula iridipennis) sample raised from the Pudukottai district of Tamilnadu was used to synthesize silver nanoparticles.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Potential Role of Propolis Nanoparticles in Medicine and Health: An Updated Review

Saad¹,

Abdel-Fattah²,

Khamis³

et al. 2021

AAVS

View full text Add to dashboard Cite

N anotechnology is the science of very tiny objects with adjacent chemical and physical structures, as well as increased reactivity and solubility. When the active constituent is nanosized, the substance's stability is boosted due to its protection from oxidizing agents, other chemicals, and enzymes (Kirtane et al., 2021). It is predicted for nanotechnology to do significant advances in medicine, as gene therapy, imaging, and novel drug discovery and drug delivery in the treatment of several diseases as diabetes, cancer, and others, in addition to advances in electronics, material science, space research, and robotics (DiSanto et al., 2015;Mohamed et al., 2021). Nanotechnology has a broad and possible impact on veterinary medicine and improves the safety of domestic animals, production, and income to the farmers by using nanomaterials. Approaches in nanoscience have the potential to solve substantial challenges in the field of animal health. It has the potential to resolve dozens of new mysteries relating to veterinary fields (Şenel, 2020).Propolis is a plant resin obtained by bees via variety of plant parts to protect the hive from insects and microbes while also maintaining the hive's appropriate temperature and humidity, it is dark yellow to brown glue and balsam (Drescher et al., 2019). Propolis contains at least 300 components, with resins (50 %), waxes (30 %), essential oils (10 %), pollen (5 %), and other organic compounds (5 %) accounting for the majority. Phenolic parts and esters, as well as various kinds of flavonoids, terpenes, steroids, aromatic beta-aldehydes, alcohols, sesquiterpenes, and stilbenes, can all be found among the organic components. The interaction of these chemicals produces a synergic effect that is crucial to propolis' biological activity. Rutin, caffeic acid phenethyl ester (CAPE), quercetin, p-coumaric review Article Abstract | Nanotechnology is defined as the science and technology of small objects with a diameter of less than 100 nanometers. Nanomaterials may undergo novel chemical and physical changes as a result of their size, indicating increased reactivity and solubility. Nanotechnology has a huge potential to make a major change in the agriculture and livestock sector. It can introduce new tools for molecular and cellular biology, biotechnology, veterinary physiology, animal genetics, and reproduction. Nano-propolis is beneficial to veterinary medicine in different aspects by varying the size of propolis using various techniques, nano-propolis can be more effective without compromising its qualities. Propolis has numerous benefits, including anti-inflammatory, antioxidant, anticancer, and antifungal properties. Low bioavailability, solubility, absorption, and untargeted control, the advantages of free form propolis are limited due to previous reasons. Nano-propolis is made using a variety of nanoencapsulation processes. Because of their smaller size, nano-propolis is more utilized by the body than the free one. This study focuses on some current research on the application ...

show abstract

Nano‐vesicular formulation of propolis and cytotoxic effects in a 3D spheroid model of lung cancer

Cited by 28 publications

References 35 publications

Biomedical Applications of Non-Small Cell Lung Cancer Spheroids

Biomedical Applications of Non-Small Cell Lung Cancer Spheroids

The matrix-dependent 3D spheroid model of the migration of non-small cell lung cancer: a step towards a rapid automated screening

Potential Role of Propolis Nanoparticles in Medicine and Health: An Updated Review

Contact Info

Product

Resources

About