Nano-SiO2 induces apoptosis via activation of p53 and Bax mediated by oxidative stress in human hepatic cell line

Ye, Yi-yi; Liu, Jianwen; Xu, Jiangang; Sun, Lijuan; Chen, Mingcang; Lan, Minbo

doi:10.1016/j.tiv.2010.01.001

Cited by 179 publications

(130 citation statements)

References 48 publications

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“…Taken together, treatment of MNP@ SiO 2 induced cytotoxicity and enhanced DNA damage in L5178Y cell. nanoparticles were found to induce oxidative stress indicated by induction of reactive oxygen species generation, and membrane lipid peroxidation (Akhtar et al, 2010), and the toxicity were confi rmed in recent studies (Nabeshi et al, 2010;Yang et al, 2010;Ye et al, 2010). It seems that these discrepancy of toxicities may be associated with nano characteristics such as size, shape, surface chemistry and degree of aggregation infl uenced the production of free radicals and oxidative stress (Aillon et al, 2009 (Yoon et al, 2005;.…”

Section: Introductionmentioning

confidence: 70%

Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Kang¹,

Yum²,

Park³

2011

Biomolecules and Therapeutics

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Section: Introductionmentioning

confidence: 70%

Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Kang¹,

Yum²,

Park³

2011

Biomolecules and Therapeutics

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“…La exposición a las NP de SiO 2 conlleva a la mayor disminución del GM-CSF en comparación de las otras NP, este efecto podría comprometer la barrera antibacteriana a nivel celular por la alteración de los neutrófilos y monocitos (13) . Se ha reportado que las NP de SiO 2 alteran la maquinaria replicativa del ADN, así como también los procesos de división celular en los epitelios de ratas, ratones, humanos y cultivos de células HaCat, L-02 y HEK293 (23)(24)(25) . Asimismo, efectos in vivo relacionados a esta NP incluyen inflamación pulmonar, daño isquémico al miocardio, viscosidad de la sangre y generación de ROS (23) .…”

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Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Zarria-Romero

Osorio

Pino

et al. 2017

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Evaluar el efecto de las nanopartículas de ZnO, TiO 2 y SiO 2 sobre la viabilidad celular y la expresión génica de las interleuquinas 7 y 3 y del factor estimulante de colonias de granulocito -macrófago (GM-CSF) en Mus musculus. Materiales y métodos. Se extrajo médula ósea roja de cinco roedores (Balb/c) para el estudio de viabilidad celular mediante la prueba de MTT. Por otro lado, grupos cinco roedores fueron inoculados vía intraperitoneal con dosis de 0,5; 1; 2,5; 5 y 10 mg/kg de nanopartículas de ZnO y SiO 2 y de 5; 10; 15; 20 y 25 mg/kg de nanopartículas deTiO 2 , 30 h después, se obtuvo el ARN a partir de la médula ósea roja para los análisis de expresión génica empleando las técnicas de PCR y RT-PCR cuantitativa. Resultados. Las nanopartículas de ZnO y SiO 2 redujeron la viabilidad celular de una manera dosis-dependiente en un 37 y 26%, respectivamente, a partir de una dosis de 1 mg/kg. En cuanto al efecto sobre la expresión génica, a las dosis 5 y 10 mg/kg, las nanopartículas de TiO 2 redujeron en mayor porcentaje la expresión de las interleuquinas 7 y 3 (55,3 y 70,2% respectivamente), con respecto a la expresión del GM-CSF, el mayor porcentaje de reducción lo produjo las nanopartículas de SiO 2 (91%). Las nanopartículas de ZnO redujeron a partir de las dosis de 20 y 25 mg/kg. Conclusiones. Las nanopartículas de ZnO, SiO 2 y TiO 2 alteran la viabilidad celular y la expresión génica en la médula ósea de ratón. Palabras clave: Nanopartículas; Interleucinas; Expresión génica; Mus musculus (fuente: DeCS BIREME). EFFECT OF THE INDUSTRIAL NANOPARTICLES TiO 2 , SiO 2 and ZnO ON CELL VIABILITY AND GENE EXPRESSION IN RED BONE MARROW OF Mus musculus ABSTRACT Objectives.To evaluate the effect of ZnO, TiO 2 and SiO 2 nanoparticles on cell viability and expression of the interleukin 7, interleukin 3, and granulocyte-macrophage colony stimulating factor (GM-CSF) genes in Mus musculus. Materials and methods. Red bone marrow was extracted from five Balb/c mice for the analysis of cell viability using the MTT test. The mice were divided into two groups of five each: one group was inoculated intraperitoneally with 0.5, 1.0, 2.5, 5.0, and 10 mg/kg of ZnO and SiO 2 nanoparticles, respectively, and the other group was inoculated with 5.0, 10.0, 15.0, 20.0, and 25 mg/kg of TiO 2 nanoparticles, respectively. Thirty hours later, RNA was extracted from the red bone marrow of the mice in both groups for gene expression analysis using quantitative PCR and RT-PCR. Results. ZnO and SiO 2 nanoparticles reduced cell viability in a dose-dependent manner by 37% and 26%, respectively, starting at a dose of 1 mg/kg. TiO 2 nanoparticles at 5 mg/kg and 10 mg/kg reduced the gene expression of interleukins 7 and 3 by 55.3% and 70.2%, respectively, and SiO 2 nanoparticles caused the greatest decrease (91%) in the expression of GM-CSF. ZnO nanoparticles reduced the expression of GM-CSF starting at doses of 20 mg/kg and 25 mg/kg. Conclusions: ZnO, SiO 2 and TiO 2 nanoparticles affect cell viability and gene expression in the mo...

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“…Mesoporous silica (MCM-41) nanoparticles are emerging as powerful nanotheranostic tools because of their porous structure, which allows them to host a large number of dye and drug molecules and because silica is considered to be safe and biodegradable. [19][20][21] While amorphous mesoporous silica nanoparticles show poor biocompatibility towards various cell types, [22][23][24] mobile composition matter of the MCM-41 type is well tolerated both in vitro and in vivo. 25 Polystyrene nanoparticles are also under evaluation for drug delivery and cellular imaging.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility, endocytosis, and intracellular trafficking of mesoporous silica and polystyrene nanoparticles in ovarian cancer cells: effects of size and surface charge groups

Ekkapongpisit¹,

Giovia²,

Follo³

et al. 2012

IJN

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Background and methods Nanoparticles engineered to carry both a chemotherapeutic drug and a sensitive imaging probe are valid tools for early detection of cancer cells and to monitor the cytotoxic effects of anticancer treatment simultaneously. Here we report on the effect of size (10–30 nm versus 50 nm), type of material (mesoporous silica versus polystyrene), and surface charge functionalization (none, amine groups, or carboxyl groups) on biocompatibility, uptake, compartmentalization, and intracellular retention of fluorescently labeled nanoparticles in cultured human ovarian cancer cells. We also investigated the involvement of caveolae in the mechanism of uptake of nanoparticles. Results We found that mesoporous silica nanoparticles entered via caveolae-mediated endocytosis and reached the lysosomes; however, while the 50 nm nanoparticles permanently resided within these organelles, the 10 nm nanoparticles soon relocated in the cytoplasm. Naked 10 nm mesoporous silica nanoparticles showed the highest and 50 nm carboxyl-modified mesoporous silica nanoparticles the lowest uptake rates, respectively. Polystyrene nanoparticle uptake also occurred via a caveolae-independent pathway, and was negatively affected by serum. The 30 nm carboxyl-modified polystyrene nanoparticles did not localize in lysosomes and were not toxic, while the 50 nm amine-modified polystyrene nanoparticles accumulated within lysosomes and eventually caused cell death. Ovarian cancer cells expressing caveolin-1 were more likely to endocytose these nanoparticles. Conclusion These data highlight the importance of considering both the physicochemical characteristics (ie, material, size and surface charge on chemical groups) of nanoparticles and the biochemical composition of the cell membrane when choosing the most suitable nanotheranostics for targeting cancer cells.

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Nano-SiO2 induces apoptosis via activation of p53 and Bax mediated by oxidative stress in human hepatic cell line

Cited by 179 publications

References 48 publications

Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Efecto de las nanopartículas industriales TiO2, SiO2 y ZnO sobre la viabilidad celular y expresión génica en médula osea roja de Mus musculus

Biocompatibility, endocytosis, and intracellular trafficking of mesoporous silica and polystyrene nanoparticles in ovarian cancer cells: effects of size and surface charge groups

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