Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Kang, Jin-Seok; Yum, Young-Na; Park, Sue-Nie

doi:10.4062/biomolther.2011.19.2.261

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…lethal DNA lesions in the form of DSBs. Also, some studies have reported that silica coated IONPs can induce cytotoxicity and DNA damage in L5178Y cells [37], A549 and HeLa cells [38] which is similar to our findings using HK-2 human renal proximal tubule epithelial cells as a model.…”

Section: Discussionsupporting

confidence: 91%

Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells

Královec

Havelek

Kročová

et al. 2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Discussionsupporting

confidence: 91%

Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells

Královec

Havelek

Kročová

et al. 2019

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…In addition to polymers, liposomes, polyelectrolyte multilayer microscapsules or micelles are typical examples of organic matrixes that can be used for encapsulation of iron oxide nanoparticles [5]. Inorganic matrixes such as silica [12] or gold [13] could also be available for the synthesis of such core-shell or embedment structure. Figure 1 illustrates MNPs with different surface functionalities.…”

Section: Overview Of Magnetic Nanoparticlesmentioning

confidence: 99%

“…More recently, much effort has been devoted to investigating the cytotoxicity of MNPs or just confirming the biocompatibility of synthesized MNPs with different surface coatings. Different surface-modified MNPs, including those coated by dextran [43][44][45], heparin [46], DMSA [29,32], APTS [32], GLU [32], protamine [47], lipid [44,45], PEG and their derivatives [33], silica [12], as well as bare MNPs [48], were evaluated. A series of targeting cell lines were used in vitro for testing including phagocytic [49], neural [48], hepatic [47], epithelial [49], stem and progenitor cells [50], immune and blood cells [49,51] and various cancer cell lines [48].…”

Section: Cytotoxicity Evaluationmentioning

confidence: 99%

In vitro biological effects of magnetic nanoparticles

Chen

2012

Chin. Sci. Bull.

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Magnetic nanoparticles (MNPs) have great potential for a wide use in various biomedical applications due to their unusual properties. It is critical for many applications that the biological effects of nanoparticles are studied in depth. To date, many disparate results can be found in the literature regarding nanoparticle-biological factors interactions. This review highlights recent developments in this field with particular focuses on in vitro MNPs-cell interactions. The effect of MNPs properties on cellular uptake and cytotoxicity evaluation of MNPs were discussed. Some employed methods are also included. Moreover, nanoparticle-cell interactions are mediated by the presence of proteins absorbed from biological fluids on the nanoparticle. Many questions remain on the effect of nanoparticle surface (in addition to nanoparticle size) on protein adsorption. We review papers related to this point too. The current development of magnetic nanoparticles (MNPs) requires a better understanding of its biological effects. A great deal of work has been conducted to study MNPsbiological factors interactions. Many researchers bend themselves to in vitro cell-based assessment. In this work, the current knowledge of how the in vitro cultured cells can interact with the exposed colloid MNPs is discussed as well as the effect of nanoparticle size and surface properties on cell responses. The specific cell line selected for in vitro assay is intended to model a response or phenomenon likely observed or sensitized by particles in vivo. Here, the frequent lack of consistency or predictability between in vitro models and in vivo observations, which is because of the different biological conditions particles exposed to and the changed cell phenotype against primary cell types, is out of our consideration. As we have known, cell monocultures as measured by in vitro assays rarely react in such isolated pathways in native tissues comprosed of multiple, dynamically communicative cell types that produce non-linear and correlated response to foreign materials.Adsorption of proteins onto the nanoparticle surface happens immediately after particles come in contact with a biological fluid, and it is the proteins associate with nanoparticles, leading to a protein "corona" which defines the biological identity of the particle. Here, we also try to review some current work on associated protein "corona" when nanoparticles were incubated in biological medium.

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“…However, despite their widespread use, research into the harmful effects of exposure on the human body is still lacking. SiO 2 NPs activate caspase‐3 and induce DNA fragmentation in human glioblastoma cells and breast cancer cells [11, 12]. In addition, it has been reported that these NPs activate proteins related to intracellular oxidative stress and apoptosis to induce toxicity [13, 14] and that the mechanism of toxicity varies depending on the cell line [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of serum protein on cell internalization of silica nanoparticles

Choi

Kim

Kwak

et al. 2022

Micro & Nano Letters

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Nanomaterials such as silica (SiO2) nanoparticles (NPs) are used in various fields owing to their unique properties, especially in biomedical applications. It has been reported that these particles induce different kinds of toxicity in various cells; therefore, it is very important to perform multifaceted analyses for accurate nanotoxicity evaluation. Most existing studies have primarily been conducted in the presence of serum, which could agglutinate SiO2 NPs, making it difficult to maintain the original size of the NPs and potentially causing errors in toxicity evaluation. Therefore, this study investigated the effects of serum proteins on the toxicity and internalization of SiO2 NPs. The toxicity of three types of monodisperse SiO2 NPs was examined in a serum‐free environment using human liver cancer (HepG2) cells and lung cancer (A549) cells. It was observed that the toxicity is significantly reduced by protein corona formation. The size‐dependent effect of SiO2 NPs on apoptosis and necrosis was also investigated, and it was observed that both apoptosis and necrosis occur under serum‐free or low‐concentration serum conditions after exposure of the cell lines to SiO2 NPs. These findings suggest that serum proteins are among the most important factors to be considered while evaluating the cytotoxicity of nanomaterials.

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Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell

Cited by 7 publications

References 25 publications

Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells

Silica coated iron oxide nanoparticles-induced cytotoxicity, genotoxicity and its underlying mechanism in human HK-2 renal proximal tubule epithelial cells

In vitro biological effects of magnetic nanoparticles

Effect of serum protein on cell internalization of silica nanoparticles

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