NanH and PknG putative virulence factors as a recombinant subunit immunogen against Corynebacterium pseudotuberculosis infection in mice

Silva, Mara Thais de Oliveira; Pinho, Rodrigo Barros de; Fonseca, Bárbara da Rocha; Bezerra, Francisco Silvestre Brilhante; Sousa, Fernanda Severo Sabedra; Seixas, Fabiana Kömmling; Collares, Tiago; Nascimento, Roberto José Meyer; Portela, Ricardo Wagner; Azevedo, Vasco; Borsuk, Sibele

doi:10.1016/j.vaccine.2020.11.010

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…Added to that, we have witnessed survival in the control group, which directly interfered with our results for the protection of challenged animals. Nonetheless, this study and previous ones [ 10 , 15 , 22 ] show that recombinant C. pseudotuberculosis proteins are promising vaccine targets that need to be further evaluated, especially if a mixture of these proteins can stimulate different immune responses, a hypothesis that should be further explored in future studies. Therefore, the use of these proteins in vaccine formulations should not be revoked.…”

Section: Discussionmentioning

confidence: 70%

“…In the study by Droppa-Almeida et al (2016), the authors tested a recombinant subunit vaccine based on the rCP40 protein against CLA, and the control group also had animals that survived [ 21 ], leading to results that were similar to the ones found here and corroborating our hypothesis for these findings. Moreover, the recombinant proteins NanH and PknG have been individually combined with the saponin adjuvant in formulations that obtained significant protection results in up to 60% of the animals [ 22 ], demonstrating that these proteins have protective potential and should be further explored as vaccine targets.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Scholl¹,

Silva²,

Barbosa³

et al. 2023

Vaccines

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Caseous lymphadenitis is a chronic contagious disease that causes economic losses worldwide. Treatments are ineffective, thus demonstrating the importance of vaccination. In this study, rNanH and rPknG proteins from Corynebacterium pseudotuberculosis were associated with saponin or aluminum hydroxide adjuvants. Three experimental groups (10 animals each) were immunized with sterile 0.9% saline solution (G1), rNanH + rPknG + Saponin (G2), rNanH + rPknG + Al(OH)3 (G3). The mice received two vaccine doses 21 days apart. Animals were challenged 21 days after the last immunization and evaluated for 50 days, with endpoint criteria applied when needed. The total IgG production levels of the experimental groups increased significantly on day 42 when compared to the control (p < 0.05). When tested against rNanH, G2 had a better rate of anti-rNanH antibodies compared to G3. In the anti-rPknG ELISA, the levels of total IgG, IgG1, and IgG2a antibodies were higher in G2. The vaccines generated partial protection, with 40% of the animals surviving the challenge. The association of recombinant NanH and PknG proteins led to promising protection rates in mice, and although using different adjuvants did not interfere with the survival rate, it influenced the immune response generated by the vaccine formulations.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Scholl¹,

Silva²,

Barbosa³

et al. 2023

Vaccines

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“…Recently, NanH and PknG were evaluated in recombinant subunit vaccines, and promoted a 60% and 20% survival rate, respectively, after mice were challenged with virulent C. pseudotuberculosis (Silva et al 2020). So, we hypothesized that these rates could be increased using improved chimeric antigens built from the rational design of immunodominant epitopes, as proposed in our study.…”

Section: Discussionmentioning

confidence: 93%

“…4), evidencing that this construct was able to maintain epitopes that are similar to the ones found on native NanH, PknG, SodC, and SpaC proteins. NanH and PknG proteins, when evaluated in recombinant subunit vaccines for CLA, had already shown good antigenic potential, as they were capable of eliciting high levels of IgG antibodies in immunized animals (Silva et al 2020). So far, SpaC and SodC proteins have not been evaluated in vaccine trials.…”

Section: Discussionmentioning

confidence: 99%

In silico analyses and design of a chimeric protein containing epitopes of SpaC, PknG, NanH, and SodC proteins for the control of caseous lymphadenitis

Silva

Pinho

Bezerra

et al. 2021

Appl Microbiol Biotechnol

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Caseous lymphadenitis (CLA) is an infectious disease that affects goats and sheep causing drastic impacts on milk and meat production and is caused by Corynebacterium pseudotuberculosis. The disease can be prevented through vaccination but currently, vaccines demonstrate limited efficacy consequently leading to a need for the development of new ones. Here, we described the in silico development of a new chimeric protein constructed with epitopes identified from the sequences of the genes nanH, pknG, spaC, and sodC, previously described as potential vaccinal targets against C. pseudotuberculosis. The chimera was expressed, purified, and its immunogenicity was evaluated using sera of immunized mice. Results indicate the chimeric protein was able to stimulate antibody production. Additionally, analysis using serum from naturally infected goats showed that the protein is recognized by sera from these animals, indicating the possibility for using this chimera in new diagnostic methods. Key points• The chimera was expressed with 52 kDa and a yield of 7 mg/L after purification.• The chimera was recognized by the sera of animals immunized with this formulation.• Chimera reacted with the serum of goats naturally infected with C. pseudotuberculosis.

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Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

Barral

Kalil

Mariutti

et al. 2022

Appl Microbiol Biotechnol

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NanH and PknG putative virulence factors as a recombinant subunit immunogen against Corynebacterium pseudotuberculosis infection in mice

Cited by 8 publications

References 45 publications

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

In silico analyses and design of a chimeric protein containing epitopes of SpaC, PknG, NanH, and SodC proteins for the control of caseous lymphadenitis

Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

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