Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

Barral, Thiago Doria; Kalil, Mauricio Alcântara; Mariutti, Ricardo Barros; Arni, R.K.; Gismene, Carolina; Sousa, Fernanda Severo Sabedra; Collares, Tiago; Seixas, Fabiana Kömmling; Borsuk, Sibele; Estrela‐Lima, Alessandra; Azevedo, Vasco; Meyer, Roberto; Portela, Ricardo Wagner

doi:10.1007/s00253-022-12279-1

Cited by 5 publications

(6 citation statements)

References 65 publications

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“…Added to that, we have witnessed survival in the control group, which directly interfered with our results for the protection of challenged animals. Nonetheless, this study and previous ones [ 10 , 15 , 22 ] show that recombinant C. pseudotuberculosis proteins are promising vaccine targets that need to be further evaluated, especially if a mixture of these proteins can stimulate different immune responses, a hypothesis that should be further explored in future studies. Therefore, the use of these proteins in vaccine formulations should not be revoked.…”

Section: Discussionmentioning

confidence: 69%

“…In these circumstances, several vaccine formulations using protein subunits came to light and were tested for their ability to produce a strong and lasting immune response [ 6 , 10 ]. Genomic studies have identified promising vaccine targets using high-throughput technologies and explored the molecular basis of these targets’ virulence and pathogenicity [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Scholl¹,

Silva²,

Barbosa³

et al. 2023

Vaccines

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Caseous lymphadenitis is a chronic contagious disease that causes economic losses worldwide. Treatments are ineffective, thus demonstrating the importance of vaccination. In this study, rNanH and rPknG proteins from Corynebacterium pseudotuberculosis were associated with saponin or aluminum hydroxide adjuvants. Three experimental groups (10 animals each) were immunized with sterile 0.9% saline solution (G1), rNanH + rPknG + Saponin (G2), rNanH + rPknG + Al(OH)3 (G3). The mice received two vaccine doses 21 days apart. Animals were challenged 21 days after the last immunization and evaluated for 50 days, with endpoint criteria applied when needed. The total IgG production levels of the experimental groups increased significantly on day 42 when compared to the control (p < 0.05). When tested against rNanH, G2 had a better rate of anti-rNanH antibodies compared to G3. In the anti-rPknG ELISA, the levels of total IgG, IgG1, and IgG2a antibodies were higher in G2. The vaccines generated partial protection, with 40% of the animals surviving the challenge. The association of recombinant NanH and PknG proteins led to promising protection rates in mice, and although using different adjuvants did not interfere with the survival rate, it influenced the immune response generated by the vaccine formulations.

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Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Scholl¹,

Silva²,

Barbosa³

et al. 2023

Vaccines

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“…Toxin inactivation could negatively impact PLD protective potential, a fact that can be exemplified by Hodsong and colleague’s experiments, where an attenuated strain called Toxminus was used for vaccine development, and PLD active site modification decreased the protective capacity of the vaccine [ 7 , 25 , 26 , 27 ]. More recently, genetic modification for the recombinant PLD active site also did not allow for reaching a survival percentage greater than 57% in vaccinated and challenged animals [ 28 ]. Other studies have used recombinant PLD as a vaccine antigen evaluated in murine models, presenting different protective percentages of 30% [ 18 ], 40% [ 29 ], and 42.86% [ 28 ] after challenge with virulence strain MIC-6 (10 4 CFU), with the common denominator being the inactivation of the toxin’s biological activity with formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic Approach of B and T Cell Epitopes of PLD and CP40 Proteins of Corynebacterium pseudotuberculosis ovis Mexican Isolate 2J-L towards a Peptide-Based Vaccine

Rodríguez-Domínguez,

Montes-de-Oca-Jiménez,

Vázquez-Chagoyán

et al. 2023

IJMS

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Mapping B and T cell epitopes constitutes an important action for peptide vaccine design. PLD and CP40 virulence factors of Corynebacterium pseudotuberculosis biovar ovis, a causal agent of Caseous Lymphadenitis, have been evaluated in a murine model as good candidates for vaccine development. Therefore, the goal of this work was to in silico analyze B and T cell epitopes of the PLD and CP40 proteins of a Mexican isolate of Corynebacterium pseudotuberculosis ovis. The Immune Epitope Data Base and Resource website was employed to predict the linear and conformational B-cell, T CD4+, and T CD8+ epitopes of PLD and CP40 proteins of Corynebacterium pseudotuberculosis ovis Mexican strain 2J-L. Fifty B cell epitopes for PLD 2J-L and forty-seven for CP40 2J-L were estimated. In addition, T CD4+ and CD8+ cell epitopes were predicted for PLD 2J-L (MHC I:16 epitopes, MHC II:10 epitopes) and CP40 2J-L (MHC I: 15 epitopes, MHC II: 13 epitopes). This study provides epitopes, paying particular attention to sequences selected by different predictor programs and overlap sequences as B and T cell epitopes. PLD 2J-L and CP40 2J-L protein epitopes may aid in the design of a promising peptide-based vaccine against Caseous Lymphadenitis in Mexico.

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“…Outro estudo elaborado por Rezende (2020) Estratégias utilizando proteínas de fusão também já foram testadas na busca por uma vacina mais eficaz. Como já eram conhecidos como alvos vacinais as proteínas PLD e CP40, em que se obteve resultados como produção significativa de anticorpos e ativação da resposta imune celular respectivamente, um estudo realizado por Barral (2022) idealizou testar uma proteína de fusão utilizando estas associadas a proteína de ligação à maltose (MBP) por esta já ter sido descrita como um adjuvante intrínseco para proteínas de fusão (GONG et al, 2015).…”

Section: Desenvolvimento De Vacinas De Subunidades Recombinantesunclassified

“…Assim foi desenvolvida a proteína de fusão MBP:PLD:CP40 para ser avaliada sua imunogenicidade e propriedades de imunoprofilaxia in silico e in vivo. Os autores concluíram que essa proteína é um antígeno potencial para imunoprofilaxia da LC pois não possui potencial alergênico e contém múltiplos epítopos que podem ser reconhecidos pelos linfócitos B e apresentados aos linfócitos T através de moléculas MHC I e II (BARRAL et al, 2022). As análises in vivo demostraram uma taxa de sobrevida de 57,14% sendo superior ao valor obtido quando testado apenas a combinação de rCP40+rPLD, que apresentou 42,86% de proteção.…”

Section: Desenvolvimento De Vacinas De Subunidades Recombinantesunclassified

Hérnia Inguino-Escrotal Congênita Em Cordeiro – Relato De Caso

Moreira,

Batista,

Souza

et al. 2024

Tecnologia E Produção Agropecuária

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Hérnia inguino-escrotal é uma patologia de origem congênita pouco relatada em cordeiros recém-nascidos. Objetivou-se escrever um caso de hérnia inguino-escrotal em cordeiro, macho, da raça Soinga. O animal, apresentou sinal clínico com tumefação na região escrotal bem característico de hérnia inguino-escrotal. O cordeiro veio a óbito dois dias após o nascimento e seu cadáver foi devidamente congelado e encaminhado para procedimento de necrópsia no Laboratório de Patologia Animal do Departamento de Ciências Animais da Universidade Federal Rural do Semiárido-UFERSA. Através do laudo anatomopatológico observou-se alterações macroscópicas na região como passagem do conteúdo abdominal através do canal inguinal, estendendo-se até a bolsa escrotal. O saco herniário continha em seu interior alças intestinais (jejuno, íleo, ceco e cólon). As alças intestinais apresentavam-se distendidas por gases, sem evidências de conteúdo em seu interior. Dessa forma, ficou confirmado o diagnóstico da causa morte por hérnia inguino-escrotal congênita.

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Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

Cited by 5 publications

References 65 publications

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Evaluation of the Association of Recombinant Proteins NanH and PknG from Corynebacterium pseudotuberculosis Using Different Adjuvants as a Recombinant Vaccine in Mice

Bioinformatic Approach of B and T Cell Epitopes of PLD and CP40 Proteins of Corynebacterium pseudotuberculosis ovis Mexican Isolate 2J-L towards a Peptide-Based Vaccine

Hérnia Inguino-Escrotal Congênita Em Cordeiro – Relato De Caso

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