Nafenopin-induced peroxisome proliferation in vitamin A deficient rats

Effects of Vitamin A Deficiency on Rat Liver Alcohol Dehydrogenase Expression and Alcohol Elimination Rate in Rats

“…Confirming the appearance of the animals, plasma and liver retinol stores were virtually absent. The levels of vitamin A in the vitamin A sufficient animals are somewhat lower than reported in some other articles, and in the chow-fed controls that we used, but this can be accounted for by the fact that the control animals were younger than those reported by Lawrence et al (1995), and the chow-fed animals had substantially higher levels of retinol in their diets.…”

“…The protocol for the production of vitamin A deficiency uses a vitamin A deficient diet fed for several weeks beginning at weaning, as previously described (Lawrence et al, 1995). This depletes any vitamin A stores that remain from the fetal and suckling periods.…”

“…We determined plasma and liver retinol levels by reversed-phase HPLC by using an internal standard method (Got et al, 1995;Lawrence et al, 1995). Two hundred microliters of serum was mixed with 800 l of saponification reagent (a 95:5:1 mixture of ethanol/KOH/pyrogallol) and saponified for 20 min at 60 o .…”

Kidney-specific Overexpression of Sirt1 Protects against Acute Kidney Injury by Retaining Peroxisome Function

“…Indeed, we have recently demonstrated that Sirt1 prevents apoptosis through the induction of catalase in HK-2 cells (7). Catalase is located in peroxisome fraction, which has been shown to promote peroxisome proliferation (26); the supplementation of peroxisome activator nafenopin results in a sustained increase in the number of peroxisomes with concomitant increases in catalase activity (27). Increased catalase therefore may eliminate H 2 O 2 within peroxisome, which possibly leads to the mitigation of the damages to peroxisome.…”

Global effects of vitamin A deficiency on gene expression in rat liver: evidence for hypoandrogenism

“…This is counterintuitive, as the PPARa/RXR heterodimer in some (but not all) cases is stimulated by the presence of 9-cis retinoic acid plus the PPARa ligand. However, other research has shown that vitamin A-deficient rats can still respond to peroxisome proliferators [48]. Hypovitaminosis A has been shown to be associated with increased plasma free fatty acids (FFAs) [49].…”