Effects of Vitamin A Deficiency on Rat Liver Alcohol Dehydrogenase Expression and Alcohol Elimination Rate in Rats

Abstract

Severe vitamin A deficiency did not alter liver ADH protein expression or rates of alcohol elimination when expressed per gram of body or liver weight.

Global effects of vitamin A deficiency on gene expression in rat liver: evidence for hypoandrogenism

“…This resulted in progressive vitamin A deficiency as the vitamin A stores present at birth and from maternal milk were depleted. As previously reported [10], growth of the vitamin A-deficient animals slowed after 31 days on the diet, suggesting the animals were vitamin-deficient for about 3 weeks before sacrifice. Total retinol in serum was at or near zero (0.5F1.6 Ag/dL) in all the animals on the vitamin A-deficient diet and was 44F6 Ag/dL for animals fed the sufficient diet [10].…”

“…As previously reported [10], growth of the vitamin A-deficient animals slowed after 31 days on the diet, suggesting the animals were vitamin-deficient for about 3 weeks before sacrifice. Total retinol in serum was at or near zero (0.5F1.6 Ag/dL) in all the animals on the vitamin A-deficient diet and was 44F6 Ag/dL for animals fed the sufficient diet [10]. Our earlier analysis of several proteins in these animals showed no difference in alcohol dehydrogenase or RXRa and an increase in RXRh [10].…”

“…Alcohol dehydrogenase type 1 and aldehyde dehydrogenase 1A1 are involved in the metabolism of retinol to retinoic acid [20,21]. Alcohol dehydrogenase mRNA increased 1.3-fold; no change in protein levels was detected in previous studies of these same animals [10]. Aldehyde dehydrogenase 1A1 increased 1.4-fold.…”

“…Total retinol in serum was at or near zero (0.5F1.6 Ag/dL) in all the animals on the vitamin A-deficient diet and was 44F6 Ag/dL for animals fed the sufficient diet [10]. Our earlier analysis of several proteins in these animals showed no difference in alcohol dehydrogenase or RXRa and an increase in RXRh [10].…”

“…The animals were sacrificed on the morning of Day 53 of the diet. These animals were described as part of an earlier study on the effect of vitamin A deficiency on liver alcohol dehydrogenase and alcohol metabolism [10]. Total liver mRNA was isolated from snap-frozen tissue using the RNeasy RNA isolation kit (Qiagen, Valencia, CA).…”