Na,K-ATPase activity and epithelial interfaces in gills of the freshwater shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

Belli, Nara Marcato; Faleiros, Rogério Oliveira; Firmino, Kelly Cristina Silva; Masui, Douglas Chodi; Leone, Francisco de Assis; McNamara, John Campbell; Furriel, Rosa dos Prazeres Melo

doi:10.1016/j.cbpa.2008.11.017

Cited by 54 publications

(63 citation statements)

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“…The principal mechanisms of ionic and osmotic regulation in freshwater crustaceans include active salt absorption across the specialized gill epithelia (Lima et al 1977, McNamara & Torres 1999, Weihrauch et al 2004, Belli et al 2009) and low water permeability that, together with the excretion of isosmotic or dilute urine by the antennal glands (Denne 1968, Augusto et al 2007a, allows the anisoionic and anisosmotic regulation of hemolymph ions and water essential to optimal cell and organ metabolism (Freire et al 2003(Freire et al , 2008. In estuarine or diadromous crustaceans that inhabit brackish or dilute media, a second mechanism, isosmotic intracellular regulation, adjusts cell volume by means of osmotic effectors like K + , small peptides and, particularly, free amino acids (FAA).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Augusto¹,

Pinheiro²,

Greene³

et al. 2009

Aquat. Biol.

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The transition from marine/brackish waters to freshwater habitats constitutes a severe osmotic and ionic challenge, and successful invasion has demanded the selection of morphological, physiological, biochemical and behavioral adaptations. We evaluated short-term (1 to 12 h exposure) and long-term (5 d acclimation), anisosmotic extracellular (osmolality, [Na + , Cl -]) and long-term isosmotic intracellular osmoregulatory capability in Palaemon northropi, a neotropical intertidal shrimp. P. northropi survives well and osmo-and ionoregulates strongly during short-and long-term exposure to 5-45 ‰ salinity, consistent with its rocky tide pool habitat subject to cyclic salinity fluctuations. Muscle total free amino acid (FAA) concentrations decreased by 63% in shrimp acclimated to 5 ‰ salinity, revealing a role in hypoosmotic cell volume regulation; this decrease is mainly a consequence of diminished glycine, arginine and proline. Total FAA contributed 31% to muscle intracellular osmolality at 20 ‰, an isosmotic salinity, and decreased to 13% after acclimation to 5 ‰. Gill and nerve tissue FAA concentrations remained unaltered. These tissue-specific responses reflect efficient anisosmotic and anisoionic extracellular regulatory mechanisms, and reveal the dependence of muscle tissue on intracellular osmotic effectors. FAA concentration is higher in P. northropi than in diadromous and hololimnetic palaemonids, confirming muscle FAA concentration as a good parameter to evaluate the degree of adaptation to dilute media. The osmoregulatory capability of P. northropi may reflect the potential physiological capacity of ancestral marine palaemonids to penetrate into dilute media, and reveals the importance of evaluating osmoregulatory processes in endeavors to comprehend the invasion of dilute media by ancestral marine crustaceans.

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Section: Introductionmentioning

confidence: 99%

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Augusto¹,

Pinheiro²,

Greene³

et al. 2009

Aquat. Biol.

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“…Los adultos de M. tenellum y M. acanthurus son hiperreguladores en bajas salinidades (<10‰) e hipoconformadores en altas salinidades, lo cual se asocia a organismos de aguas salobres (Signoret et al, 1997). Los adultos de M. amazonicum hiperosmorregulan a 20‰ (Belli et al, 2009), en cambio los adultos de M. rosenbergii son hiperreguladores hasta en 14‰ y osmoconformadores entre 14.5‰ a 21‰ de salinidad como estrategia para disminuir el gradiente osmótico entre la hemolinfa y el medio externo (Cheng et al, 2003;Huong et al, 2010) y en éstas salinidades hay expresión de genes en las branquias que permiten tolerar dichas salinidades (Barman et al, 2012). Sin embargo, no se conoce la osmorregulación de ningún estado ontogenético de C. caementarius, pero dado su comportamiento migratorio de los machos adultos, deben poseer mecanismos osmorregulatorios para soportar cierta concentración de salinidad, cuando en algún momento de su vida se encuentren en contacto con agua salobre, similar a lo sucedido en hembras de la especie, que incrementan los hemocitos y la concentración de oxihemocianina, con el incremento de la salinidad del medio a 6‰ y hasta 12‰ (Valverde et al, 2014).…”

Section: Introductionunclassified

SUPERVIVENCIA Y CRECIMIENTO DE MACHOS ADULTOS DEL CAMARÓN DE RÍO Cryphiops caementarius Molina, 1782 (CRUSTACEA, PALAEMONIDAE) EXPUESTOS A SALINIDADES

2017

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ResumenEl objetivo fue evaluar la supervivencia y el crecimiento de machos adultos del camarón de río Cryphiops caementarius expuestos a salinidades. El experimento se realizó en 12 acuarios con 50 l de agua, cada uno con filtro biológico y con cinco refugios. Las salinidades experimentales fueron 0, 2, 4 y 8‰, implementadas por triplicado. En cada acuario se sembró al azar cinco camarones. Un total de 60 camarones machos adultos (5.94 ± 0.11 cm de longitud total y 8.15 ± 0.810 g de peso húmedo) fueron usados en el experimento que duró 60 días. Los camarones expuestos en agua con 4‰ de salinidad presentaron alta y significativa (p<0.05) supervivencia (>80%), en cambio en 0‰ y 2‰ la supervivencia fue baja (20%) debido al canibalismo y en 8‰ la supervivencia fue muy baja (6%) debido al estrés osmótico. El crecimiento de los camarones expuestos en salinidad de 4‰ no mostró diferencia significativa (p>0.05) con aquellos expuestos en 0‰ y en 2‰ de salinidad durante el período de estudio, pero los mayores valores de crecimiento en peso se presentaron en 4‰ de salinidad. Palabras clave: estrés osmótico, crecimiento, osmorregulación, supervivencia, Cryphiops. AbstractThe aim was to evaluate the survival and growth of adult males of the freshwater shrimp Cryphiops caementarius exposed to salinities. The experiment was carried out in 12 aquariums with 50 l of water, each with biological filter and five shelters. The experimental salinities were 0, 2, 4 and 8‰, implemented in triplicate. Five shrimps were stocked at random in each aquarium. A total of 60 adult male shrimps (5.94 ± 0.11 cm of total length and 8.15 ± 0.810 g of wet weight) were used in the experiment that lasted 60 days. Shrimps exposed to water with 4‰ of salinity presented high and significant (p<0.05) survival (>80%), whereas in 0 ‰ and 2 ‰ the survival was low (20%) due to cannibalism, and in 8‰ the survival was very low (6%) due to osmotic stress. Growth of shrimps exposed to 4‰ salinity showed no significant difference (p>0.05) with those exposed to 0‰ and 2‰ of salinity during the study period, but the highest values of growth in weight were presented to 4‰ salinity.

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“…O uso de p-nitrofenilfosfato como substrato para caracterizar cineticamente a atividade de K + -fosfatase da enzima das brânquias de crustáceos provou ser uma alternativa conveniente para estudos comparativos da atividade (Na + ,K + )-ATPase não só em M. olfersi (FURRIEL et al , 2001, MENDONÇA et al, 2007, mas também no caranguejo Portunidae, C. danae (MASUI et al, 2003;MASUI et al, 2005b), no camarão M. amazonicum (BELLI et al, 2009), e no ermitão Clibanarius symmetricus . A caracterização cinética da atividade K + -fosfatase em M. amazonicum juvenil e adulto ajudou a elucidar a capacidade osmoregulatória nestes dois estágios do ciclo de vida (LEONE et al, 2013 (FURRIEL et al, 2001MASUI et al, 2005;MENDONÇA et al, 2007;BELLI et al, 2009;GARÇON et al, 2012;GARÇON et al, 2013;LEONE et al, 2013).…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…A caracterização cinética da atividade K + -fosfatase em M. amazonicum juvenil e adulto ajudou a elucidar a capacidade osmoregulatória nestes dois estágios do ciclo de vida (LEONE et al, 2013 (FURRIEL et al, 2001MASUI et al, 2005;MENDONÇA et al, 2007;BELLI et al, 2009;GARÇON et al, 2012;GARÇON et al, 2013;LEONE et al, 2013). O valor de atividade máxima observado para C. guanhumi foi menor quando comparados aos camarões M. amazonicum e M. olfersii, ao siri C. danae e ao ermitão Clibanarius vittatus (BELLI et al, 2009;LEONE et al, 2012;FURRIEL et al, 2001;MASUI et al, 2003;GARÇON et al, 2012).…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…Os íons magnésio são essenciais para a atividade K + -fosfatase da (Na + ,K + )-ATPase de diferentes fontes (ROSSI et al, 1978;GACHE et al, 1979;GLYNN, 1985;ROBINSON & PRATAP, 1991;BERBERIAN & BEAUGÉ, 1992;GATTO et al, 2007) e a inibição da atividade em concentrações elevadas destes íons pode ser atribuída à competição com os íons K + pela ligação aos sítios de transporte, na molécula da enzima (GATTO et al, 2007 (FURRIEL et al, 2001;MASUI et al, 2003;MASUI et al, 2005;MENDONÇA et al, 2007;BELLI et al, 2009;GARÇON et al, 2007GARÇON et al, e 2009 (MASUI et al, 2005(MASUI et al, , 2003. Valores próximos foram relatados para a (Na + ,K + )-ATPase de vertebrados ( ROBINSON, 1981;GLYNN, 1985).…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

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Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

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Na,K-ATPase activity and epithelial interfaces in gills of the freshwater shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

Cited by 54 publications

References 55 publications

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

Evolutionary transition to freshwater by ancestral marine palaemonids: evidence from osmoregulation in a tide pool shrimp

SUPERVIVENCIA Y CRECIMIENTO DE MACHOS ADULTOS DEL CAMARÓN DE RÍO Cryphiops caementarius Molina, 1782 (CRUSTACEA, PALAEMONIDAE) EXPUESTOS A SALINIDADES

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

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