N-starvation stress induced FUM gene expression and fumonisin production is mediated via the HOG-type MAPK pathway in Fusarium proliferatum

Integrating toxin gene expression, growth and fumonisin B 1 and B 2 production by a strain of Fusarium verticillioides under different environmental factors

“…The function of the others is not known yet. Few studies have investigated the relationship between the fumonisin production, FUM gene expression and ecological factors [13,14,[30][31][32]. Here, we developed ternary diagrams to examine the link between the FUM1 a key gene and eight other pathway genes (FUM7, FUM10, FUM11, FUM12, FUM13, FUM14, FUM16 and FUM19) in relation to environmental conditions and fumonisin synthesis.…”

FUM and BIK gene expression contribute to describe fumonisin and bikaverin synthesis in Fusarium verticillioides

“…Kohut et al (2009) found that the HOG-type MAP kinase (MAPK) influenced both the expression of FUM1 and FUM8 and FB 1 production during N-starvation; moreover Ádám et al (2008) observed that Fphog1, a HOG-type MAPK gene cloned from F. proliferatum, was not involved in transcriptional regulation under salt and hyperosmolar stress conditions. A similar role of HOG-type MAPK in F. verticillioides could be hypothesized and it would be interesting to study the HOG response in F. verticillioides cultures.…”

“…It was reported that nitrogen starvation resulted in induced FUM1 and FUM8 expression and increased fumonisin production in both F. proliferatum (Kohut et al, 2009) and F. verticillioides, suggesting that fumonisin biosynthesis is regulated by the global nitrogen regulatory gene AREA (Kim and Woloshuk, 2008). AREA is postulated to regulate BIK gene expression in F. fujikuroi as well (reviewed in Limón et al, 2010).…”

Role of MAP Kinase Signaling in Secondary Metabolism and Adaptation to Abiotic/Fungicide Stress in Fusarium

“…Nitrogen depletion induced the production of fumonisin B1, a polyketide derivative mycotoxin and increased the expression of fuminisin biosynthesis genes in F. proliferatum. Under nitrogen starvation (absence of any N-source) conditions deletion of Fphog1, a HOG-type MAP kinase gene resulted in further increases in FUM1 and FUM8 gene expression, as well as fumonisin B1 production suggesting that this response is mediated via the HOG-type MAPK pathway in F. proliferatum (Kohut et al, 2009). In a more recent study Fvmk1, a PMK-type MAPK gene was identified as a positive regulator of fumonisin B1 production in F. verticillioides (Zhang et al, 2011).…”

“…In addition to its role in stress and fungicide tolerance, the HOG1 MAPK pathway plays also an important role in the regulation of secondary metabolism in different Fusarium species (Ochiai et al, 2007;Kohut et al, 2009). Disruption of either Fgos2 (a HOG-type MAPK orthologue) or Fgos4 (encoding a MAPK kinase) or Fgos5 (encoding a MAPK kinase kinase) blocked trichotecene production in F. graminearum and substantially reduced expression of the trichotecene gene cluster.…”