N,N,N-Trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen

Subbiah, Ramesh; Ramalingam, Prakash; Ramasundaram, Subramaniyan; Kim, Do Yang; Park, Kwideok; Ramasamy, Mohankandhasamy; Choi, Kyoung Jin

doi:10.1016/j.carbpol.2012.04.056

Cited by 80 publications

(30 citation statements)

References 30 publications

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“…TMC is a biocompatible and biodegradable polymer which can be effectively used to form NPs of 150-200 nm via ionic gelation process with tripolyphosphate (TPP) as an anionic counterpart (10). Previously, many therapeutic moieties including antioxidants, enzymes, vaccines, antimicrobials and small molecules were successfully encapsulated and administered in vivo (11). The encapsulation of PTX in TMC NPs could effectively overcome the solubility issues, provide a controlled release profile and can prolong the half-life of drug in the in vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

Song

Cheng

et al. 2014

Oncology Reports

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Gastroenteric cancer is one of the most prevalent cancers and is responsible for most cancer-related deaths worldwide. Paclitaxel (PTX), a classical microtubule inhibitor, is indicated in the treatment of gastric/gastroenteric cancers. In the present study, trimethyl chitosan (TMC)-loaded PTX (TMC-PTX) was prepared and evaluated for its therapeutic effect in gastric cancers. A spherical shaped nanosized TMC-PTX particle was formed with high loading capacity (~30%) for PTX. The nanoparticles (NPs) showed a sustained release pattern (~70%) for up to 96 h of study period. The positively charged NPs were preferentially internalized by Caco-2 cells. TMC-PTX inhibited the gastric cell proliferation with an IC50 value of 0.6 µg in NCI-N87 cells while it was 1.26 µg in the SGC-7901 cell line after 24 h exposure. The apoptosis assay (Annexin V/PI) showed a large presence of cells in the early and late apoptosis chamber, while cell cycle analysis showed a marked G2/M phase arrest (50-60%) in NCI-N87 and SGC-7901 cell lines indicating its potent anti-proliferative effect. The in vivo antitumor study in NCI-N87 and SGC-7901 bearing xenograft model showed a superior chemotherapeutic efficacy for TMC-PTX NP. The NP group significantly reduced the tumor growth with no obvious sign of systemic side-effects (safety). Collectively, our results suggest that the microtubule inhibitory effect of PTX-loaded polymer NP could be a promising system for the treatment of gastroenteric cancers.

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Section: Introductionmentioning

confidence: 99%

Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

Song

Cheng

et al. 2014

Oncology Reports

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“…[26][27][28] Previous studies have shown that chitosan (CS) and TMC NPs are efficient vaccine delivery systems for systemic vaccination. [29][30][31][32] In another study, Slutter et al compared CS and TMC NPs' function in oral immunization. They had indicated that application of TMC NPs is a promising strategy for oral immunization.…”

Section: Discussionmentioning

confidence: 99%

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

Abkar¹,

Fasihi-Ramandi²,

Kooshki³

et al. 2017

IJN

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Brucellosis is a group of closely associated zoonotic bacterial illnesses caused by members of the genus Brucella. B. melitensis Omp31 is a promising candidate for a subunit vaccine against brucellosis. This study surveyed the immunogenicity of Omp31 alone and with incomplete Freund’s adjuvant (Omp31-IFA) and N -trimethyl chitosan (TMC/Omp31) nanoparticles (NPs), as well as the effect of Omp31 immunization route on immunological responses and protection. After expression and purification, the recombinant Omp31 (rOmp31) was loaded onto TMC NPs by ionic gelation. The particle size, loading efficiency and in vitro release of the NPs were examined. Omp31-IFA was administered intraperitoneally, while TMC/Omp31 NPs were administered orally and intraperitoneally. According to the antibody subclasses and cytokine profile, intraperitoneal immunization by Omp31-IFA and TMC/Omp31 NPs induced T helper 1 (Th1) and Th1–Th2 immune responses, respectively. On the other hand, oral immunization with TMC/Omp31 NPs elicited a mixed Th1–Th17 immune response. Data obtained from the cell proliferation assay showed that vaccination with Omp31 stimulated a vigorous antigen-specific cell proliferative response, which could be further increased after oral immunization with TMC/Omp31 NPs. Vaccinated groups of mice when challenged with B. melitensis 16M were found to be significantly protected in the orally administered group in comparison with the intraperitoneally immunized mice. Results of this study indicated that the reason for high protection after oral vaccination can be via elicited Th17 response.

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“…CHT uses are limited, although there are many studies on the use of CHT as drug carriers. This fact occurs due to its low solubility at pH 7.4 [14], reduced mucoadhesion and a poor membrane cell permeation above pH 6.5 [1,17,18]. Combining or preparing CHT-derivatives is a rational pathway to overcome CHT solubility restrictions generating new materials with wider applications than those presented by conventional CHT-based materials [19,20].…”

Section: Introductionmentioning

confidence: 98%

“…Combining or preparing CHT-derivatives is a rational pathway to overcome CHT solubility restrictions generating new materials with wider applications than those presented by conventional CHT-based materials [19,20]. N,N,N-Trimethyl chitosan (TMC), the simplest form of quaternized CHT, has greater antimicrobial action, mucoadhesivity and permeability in relation to CHT and it is water soluble even at pH > 7 [1,17]. Follmann et al [21] developed antimicrobial and antiadhesive TMC/HP films by LbL on chemically modified polystyrene substrates.…”

Section: Introductionmentioning

confidence: 99%

N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

Bueno

Souza

Follmann

et al. 2015

International Journal of Biological Macromolecules

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Polysaccharide-based device for oral delivery of heparin (HP) was successfully prepared. Previously synthesized N,N-dimethyl chitosan (DMC) (86% dimethylated by (1)H NMR spectroscopy) was complexed with HP by mixing HP and DMC aqueous solutions (both at pH 3.0). The polyelectrolyte complex (PEC) obtention was confirmed by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA/DTG) and wide-angle X-ray scattering (WAXS). In vitro controlled release assays of HP from PEC were investigated in the simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). The PEC efficiently protected the HP in SGF condition in which HP is degraded. On the other hand, in SIF PEC promoted the releasing of 80 ± 1.5% of loaded HP. The promissory results indicated that the PEC based on DMC/HP presented potential as drug-carrier matrix, since biological activity of HP was improved at pH close to physiological condition.

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N,N,N-Trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen

Cited by 80 publications

References 30 publications

Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

Oral immunization of mice with Omp31-loaded <em>N</em>-trimethyl chitosan nanoparticles induces high protection against <em>Brucella melitensis</em> infection

N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

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