N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

Bueno, Pedro V.A.; Souza, Paulo R.; Follmann, Heveline D.M.; Pereira, Antonio G.B.; Martins, Alessandro F.; Rubira, Adley F.; Muniz, Edvani C.

doi:10.1016/j.ijbiomac.2015.01.030

Cited by 44 publications

(16 citation statements)

References 39 publications

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“…These polymers are not absorbed in the GI tract due to their high molecular weight. Three mucoadhesive polymers, chitosan derivatives, poly(acrylates) and thiolated polymers, were described to increase the absorption of heparins in GI tract [ 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Co-administration With Penetration Enhancersmentioning

confidence: 99%

“…The absorption of reviparin was assessed by measuring the anti-FXa plasma levels and an increase in plasma anti-FXa levels to 0.4 anti-Xa U/mL was observed after administration of SNOCC at 3% ( w / v ) with reviparin. Recently, another chitosan derivative, N , N -dimethyl chitosan (DMC, Figure 5 ), was used to form a polyelectrolyte complex with UFH [ 63 ]. In vitro release studies showed that heparin is released from the complex which indicates that DMC could be used to increase the oral bioavailability of heparin.…”

Section: Co-administration With Penetration Enhancersmentioning

confidence: 99%

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Strategies to Overcome Heparins’ Low Oral Bioavailability

et al. 2016

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Even after a century, heparin is still the most effective anticoagulant available with few side effects. The poor oral absorption of heparins triggered the search for strategies to achieve oral bioavailability since this route has evident advantages over parenteral administration. Several approaches emerged, such as conjugation of heparins with bile acids and lipids, formulation with penetration enhancers, and encapsulation of heparins in micro and nanoparticles. Some of these strategies appear to have potential as good delivery systems to overcome heparin’s low oral bioavailability. Nevertheless, none have reached the market yet. Overall, this review aims to provide insights regarding the oral bioavailability of heparin.

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Section: Co-administration With Penetration Enhancersmentioning

confidence: 99%

Section: Co-administration With Penetration Enhancersmentioning

confidence: 99%

Strategies to Overcome Heparins’ Low Oral Bioavailability

et al. 2016

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“…32 Due to the pH-response property, drugs were also delivered to the inflammatory regions and infected areas. 30,31 In addition, in view of specific normal physiological microenvironments such as intestinal fluid (SIF, pH 6.8) and simulated gastric fluid (SGF, pH 1.2), 38 drug delivery systems for the gastrointestinal tract have also been developed. 39 Based on the different acidity/alkalinity microenvironment of the body for any situation, physiological or pathological, various CUR pH-sensitive preparations (liposomes, nanoparticle, micelles, microspheres) were developed to enhance the targeting ability and treatment effect of CUR ( Figure 3).…”

Section: Ph-sensitive Preparationmentioning

confidence: 99%

<p>Review of Curcumin Physicochemical Targeting Delivery System</p>

Li¹,

Zhang²,

Pi³

et al. 2020

IJN

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“…Type I collagen forms more than 90% of the organic mass of bone tissue in the human body and is majorly prevalent in most other tissues in the human body . Heparin, the second polymer used in our work, is a polyelectrolyte and the strongest negatively charged organic substance in the human body . Heparin plays important roles in the human body; notably, heparin molecules are involved in binding and potentiating several important growth factors in the extracellular matrix. − Heparin − and collagen ,− have been used in several studies that use layer-by-layer assembly.…”

Section: Introductionmentioning

confidence: 99%

Methods for the Assembly and Characterization of Polyelectrolyte Multilayers as Microenvironments to Modulate Human Mesenchymal Stromal Cell Response

Castilla‐Casadiego

Timsina

Haseli

et al. 2020

ACS Biomater. Sci. Eng.

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Thin films are of interest in materials design because they allow for the modification of surface properties of materials while the bulk properties of the material are largely unaffected. In this work, we outline methods for the assembly of thin films using a technique known as layer-by-layer (LbL). Furthermore, their interactions with human mesenchymal stromal cells (hMSCs) are discussed. hMSCs are a subject of growing interest because of their potential to treat or cure diseases, given their immunosuppressive properties, multipotent differentiation capabilities, and tissue regeneration capabilities. Numerous improvements and modifications have been suggested for the harvesting, treatment, and culture of hMSCs prior to their administration in human subjects. Here, we discuss methods to assess the interactions of hMSCs with thin LbL-assembled films of heparin and collagen. Three different methods are discussed. The first details the preparation of heparin/collagen multilayers on different surfaces and the seeding of cells on these multilayers. The second method details the characterization of multilayers, including techniques to assess the thickness, roughness, and surface charge of the multilayers, as well as in situ deposition of multilayers. The third method details the analysis of cell interactions with the multilayers, including techniques to assess proliferation, viability, real-time monitoring of hMSC behavior, analysis of hMSC-adhesive proteins on the multilayers, immunomodulatory factor expression of hMSCs, and cytokine expression on heparin/collagen multilayers. We propose that the methods described in this work will assist in the design and characterization of LbL-assembled thin films and the analysis of hMSCs cultured on these thin films.

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N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

Cited by 44 publications

References 39 publications

Strategies to Overcome Heparins’ Low Oral Bioavailability

Strategies to Overcome Heparins’ Low Oral Bioavailability

<p>Review of Curcumin Physicochemical Targeting Delivery System</p>

Methods for the Assembly and Characterization of Polyelectrolyte Multilayers as Microenvironments to Modulate Human Mesenchymal Stromal Cell Response

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